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Open AccessArticle

Low Temperature Dissolution of Yeast Chitin-Glucan Complex and Characterization of the Regenerated Polymer

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UCIBIO-REQUIMTE, Chemistry Department, Faculty of Science and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal
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LEAF—Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, Portugal
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i3N/CENIMAT, Department of Materials Science, Faculty of Science and Technology, NOVA University of Lisbon and CEMOP/UNINOVA, Campus da Caparica, 2829-516 Caparica, Portugal
*
Author to whom correspondence should be addressed.
Bioengineering 2020, 7(1), 28; https://doi.org/10.3390/bioengineering7010028
Received: 13 February 2020 / Revised: 9 March 2020 / Accepted: 9 March 2020 / Published: 14 March 2020
Chitin-glucan complex (CGC) is a copolymer composed of chitin and glucan moieties extracted from the cell-walls of several yeasts and fungi. Despite its proven valuable properties, that include antibacterial, antioxidant and anticancer activity, the utilization of CGC in many applications is hindered by its insolubility in water and most solvents. In this study, NaOH/urea solvent systems were used for the first time for solubilization of CGC extracted from the yeast Komagataella pastoris. Different NaOH/urea ratios (6:8, 8:4 and 11:4 (w/w), respectively) were used to obtain aqueous solutions using a freeze/thaw procedure. There was an overall solubilization of 63–68%, with the highest solubilization rate obtained for the highest tested urea concentration (8 wt%). The regenerated polymer, obtained by dialysis of the alkali solutions followed by lyophilization, formed porous macrostructures characterized by a chemical composition similar to that of the starting co-polymer, although the acetylation degree decreased from 61.3% to 33.9–50.6%, indicating that chitin was converted into chitosan, yielding chitosan-glucan complex (ChGC). Consistent with this, there was a reduction of the crystallinity index and thermal degradation temperature. Given these results, this study reports a simple and green procedure to solubilize CGC and obtain aqueous ChGC solutions that can be processed as novel biomaterials. View Full-Text
Keywords: chitin-glucan complex (CGC); chitosan-glucan complex (ChGC); NaOH/urea solvent systems; dissolution; structural analysis; thermal properties chitin-glucan complex (CGC); chitosan-glucan complex (ChGC); NaOH/urea solvent systems; dissolution; structural analysis; thermal properties
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MDPI and ACS Style

Araújo, D.; Alves, V.D.; Marques, A.C.; Fortunato, E.; Reis, M.A.M.; Freitas, F. Low Temperature Dissolution of Yeast Chitin-Glucan Complex and Characterization of the Regenerated Polymer. Bioengineering 2020, 7, 28. https://doi.org/10.3390/bioengineering7010028

AMA Style

Araújo D, Alves VD, Marques AC, Fortunato E, Reis MAM, Freitas F. Low Temperature Dissolution of Yeast Chitin-Glucan Complex and Characterization of the Regenerated Polymer. Bioengineering. 2020; 7(1):28. https://doi.org/10.3390/bioengineering7010028

Chicago/Turabian Style

Araújo, Diana; Alves, Vítor D.; Marques, Ana C.; Fortunato, Elvira; Reis, Maria A.M.; Freitas, Filomena. 2020. "Low Temperature Dissolution of Yeast Chitin-Glucan Complex and Characterization of the Regenerated Polymer" Bioengineering 7, no. 1: 28. https://doi.org/10.3390/bioengineering7010028

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