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Open AccessArticle

Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium

1
Products and Processes for Biotechnology Group, Engineering and Technology Institute Groningen, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
2
Paques Technology B.V., Tjalke de Boerstrjitte 24, 8561 EL Balk, The Netherlands
3
Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Bioengineering 2020, 7(1), 19; https://doi.org/10.3390/bioengineering7010019
Received: 22 January 2020 / Revised: 18 February 2020 / Accepted: 19 February 2020 / Published: 21 February 2020
Polyhydroxyalkanoates (PHAs) form a highly promising class of bioplastics for the transition from fossil fuel-based plastics to bio-renewable and biodegradable plastics. Mixed microbial consortia (MMC) are known to be able to produce PHAs from organic waste streams. Knowledge of key-microbes and their characteristics in PHA-producing consortia is necessary for further process optimization and direction towards synthesis of specific types of PHAs. In this study, a PHA-producing mixed microbial consortium (MMC) from an industrial pilot plant was characterized and further enriched on acetate in a laboratory-scale selector with a working volume of 5 L. 16S-rDNA microbiological population analysis of both the industrial pilot plant and the 5 L selector revealed that the most dominant species within the population is Thauera aminoaromatica MZ1T, a Gram-negative beta-proteobacterium belonging to the order of the Rhodocyclales. The relative abundance of this Thauera species increased from 24 to 40% after two months of enrichment in the selector-system, indicating a competitive advantage, possibly due to the storage of a reserve material such as PHA. First experiments with T. aminoaromatica MZ1T showed multiple intracellular granules when grown in pure culture on a growth medium with a C:N ratio of 10:1 and acetate as a carbon source. Nuclear magnetic resonance (NMR) analyses upon extraction of PHA from the pure culture confirmed polyhydroxybutyrate production by T. aminoaromatica MZ1T. View Full-Text
Keywords: Thauera aminoaromatica MZ1T; biopolymers; polyhydroxyalkanoate (PHA); polyhydroxybutyrate (PHB); 16S-rDNA analysis; biodiversity; mixed microbial consortium Thauera aminoaromatica MZ1T; biopolymers; polyhydroxyalkanoate (PHA); polyhydroxybutyrate (PHB); 16S-rDNA analysis; biodiversity; mixed microbial consortium
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MDPI and ACS Style

Colpa, D.I.; Zhou, W.; Wempe, J.P.; Tamis, J.; Stuart, M.C.A.; Krooneman, J.; Euverink, G.-J.W. Thauera aminoaromatica MZ1T Identified as a Polyhydroxyalkanoate-Producing Bacterium within a Mixed Microbial Consortium. Bioengineering 2020, 7, 19.

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