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Open AccessArticle

In-Line Monitoring of Polyhydroxyalkanoate (PHA) Production during High-Cell-Density Plant Oil Cultivations Using Photon Density Wave Spectroscopy

1
Bioprocess Engineering, Department of Biotechnology, Technische Universität Berlin, 13355 Berlin, Germany
2
innoFSPEC, University of Potsdam, 14476 Potsdam, Germany
*
Author to whom correspondence should be addressed.
Bioengineering 2019, 6(3), 85; https://doi.org/10.3390/bioengineering6030085
Received: 29 August 2019 / Revised: 12 September 2019 / Accepted: 17 September 2019 / Published: 19 September 2019
(This article belongs to the Special Issue Advances in Polyhydroxyalkanoate (PHA) Production, Volume 2)
Polyhydroxyalkanoates (PHAs) are biodegradable plastic-like materials with versatile properties. Plant oils are excellent carbon sources for a cost-effective PHA production, due to their high carbon content, large availability, and comparatively low prices. Additionally, efficient process development and control is required for competitive PHA production, which can be facilitated by on-line or in-line monitoring devices. To this end, we have evaluated photon density wave (PDW) spectroscopy as a new process analytical technology for Ralstonia eutropha (Cupriavidus necator) H16 plant oil cultivations producing polyhydroxybutyrate (PHB) as an intracellular polymer. PDW spectroscopy was used for in-line recording of the reduced scattering coefficient µs’ and the absorption coefficient µa at 638 nm. A correlation of µs’ with the cell dry weight (CDW) and µa with the residual cell dry weight (RCDW) was observed during growth, PHB accumulation, and PHB degradation phases in batch and pulse feed cultivations. The correlation was used to predict CDW, RCDW, and PHB formation in a high-cell-density fed-batch cultivation with a productivity of 1.65 gPHB·L−1·h−1 and a final biomass of 106 g·L−1 containing 73 wt% PHB. The new method applied in this study allows in-line monitoring of CDW, RCDW, and PHA formation. View Full-Text
Keywords: polyhydroxyalkanoate; PHA; process analytical technologies; PAT; plant oil; high-cell-density fed-batch; photon density wave spectroscopy; PDW; Ralstonia eutropha; Cupriavidus necator; on-line; in-line polyhydroxyalkanoate; PHA; process analytical technologies; PAT; plant oil; high-cell-density fed-batch; photon density wave spectroscopy; PDW; Ralstonia eutropha; Cupriavidus necator; on-line; in-line
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MDPI and ACS Style

Gutschmann, B.; Schiewe, T.; Weiske, M.T.; Neubauer, P.; Hass, R.; Riedel, S.L. In-Line Monitoring of Polyhydroxyalkanoate (PHA) Production during High-Cell-Density Plant Oil Cultivations Using Photon Density Wave Spectroscopy. Bioengineering 2019, 6, 85.

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