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Bioengineering 2019, 6(2), 28; https://doi.org/10.3390/bioengineering6020028

Microwave Pretreatment and Enzymolysis Optimization of the Lotus Seed Protein

1
Biotechnology Institute, College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan, China
2
Biology and Chemical Engineering School, Panzhihua University, Panzhihua 617000, Sichuan, China
*
Author to whom correspondence should be addressed.
Received: 3 March 2019 / Revised: 19 March 2019 / Accepted: 24 March 2019 / Published: 27 March 2019
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PDF [1203 KB, uploaded 27 March 2019]
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Abstract

Pretreatment with a microwave was conducted before enzymolysis and shown to enhance the enzymolysis, which changed the secondary structure of the lotus seed protein. Under high-power microwave irradiation, sub bonds of the protein were broken, causing disaggregation and unfolding of the secondary structure, namely a decrease in the intermolecular aggregate structure and increase in the random coil structure, making the protein bonds susceptible to papain in the enzymolysis. On the other hand, a response surface methodology (RSM) was launched to investigate the influence of the enzymolysis process variables on the DH (degree of hydrolysis). The statistical analysis revealed that the optimized conditions were a protein substrate concentration of 15 g/L, pH of 5.5, enzymolysis temperature of 57 °C, papain amount of 0.5 g/L, and enzymolysis time of 45 min, for which the predicted value of the DH was 35.64%. The results indicated that a microwave also had better potential for applications in the enzymolysis of foods. View Full-Text
Keywords: microwave; lotus seed protein; irradiation powers; structural changes; response surface method; hydrolysis degree microwave; lotus seed protein; irradiation powers; structural changes; response surface method; hydrolysis degree
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Gohi, B.F.C.A.; Du, J.; Zeng, H.-Y.; Cao, X.-J.; Zou, K. Microwave Pretreatment and Enzymolysis Optimization of the Lotus Seed Protein. Bioengineering 2019, 6, 28.

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