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Bioengineering 2019, 6(1), 8; https://doi.org/10.3390/bioengineering6010008

Characterization of Plasma-Derived Extracellular Vesicles Isolated by Different Methods: A Comparison Study

1
Department of Chemical and Enviromental Engineering, Faculty of Chemistry, University of Oviedo, 33006 Oviedo, Spain
2
Department of Analytical and Physical Chemistry, Faculty of Chemistry, University of Oviedo, 33006 Oviedo, Spain
3
Department of Physics, Gijón Polytechnic School of Engineering, University of Oviedo, 33006 Oviedo, Spain
4
Neurology Department, Hospital Universitario Central de Asturias, 33011 Oviedo, Spain
5
Department of Morphology and Cellular Biology, Instituto de Neurociencias del Principado de Asturias (INEUROPA), University of Oviedo, 33006 Oviedo, Spain
*
Authors to whom correspondence should be addressed.
Received: 30 November 2018 / Revised: 8 January 2019 / Accepted: 11 January 2019 / Published: 17 January 2019
(This article belongs to the Special Issue Extracellular Vesicles: From Biology to Biomedical Applications)
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Abstract

Extracellular vesicles (EV) are small membrane structures released by cells that act as potent mediators of intercellular communication. The study of EV biology is important, not only to strengthen our knowledge of their physiological roles, but also to better understand their involvement in several diseases. In the field of biomedicine they have been studied as a novel source of biomarkers and drug delivery vehicles. The most commonly used method for EV enrichment in crude pellet involves serial centrifugation and ultracentrifugation. Recently, different protocols and techniques have been developed to isolate EV that imply less time and greater purification. Here we carry out a comparative analysis of three methods to enrich EV from plasma of healthy controls: ultracentrifugation, ExoQuickTM precipitation solution (System Biosciences), and Total Exosome Isolation kit (Invitrogen). Our results show that commercial precipitation reagents are more efficient and enable higher EV enrichment factors compared with traditional ultracentrifugation, although subsequent imaging analysis is not possible with some of them. We hope that this work will contribute to the current research on isolation techniques to assist the progress of clinical applications with diagnostic or therapeutic objectives. View Full-Text
Keywords: extracellular vesicles; enrichment; ultracentrifugation; nanoparticle tracking analysis; lateral flow immunoassay extracellular vesicles; enrichment; ultracentrifugation; nanoparticle tracking analysis; lateral flow immunoassay
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Serrano-Pertierra, E.; Oliveira-Rodríguez, M.; Rivas, M.; Oliva, P.; Villafani, J.; Navarro, A.; Blanco-López, M.C.; Cernuda-Morollón, E. Characterization of Plasma-Derived Extracellular Vesicles Isolated by Different Methods: A Comparison Study. Bioengineering 2019, 6, 8.

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