Next Article in Journal
Microfabricated and 3-D Printed Soft Bioelectronic Constructs from PAn-PAAMPSA-Containing Hydrogels
Next Article in Special Issue
Thermoplastic PCL-b-PEG-b-PCL and HDI Polyurethanes for Extrusion-Based 3D-Printing of Tough Hydrogels
Previous Article in Journal
Palliative Care for Children with Central Nervous System Malignancies
Previous Article in Special Issue
Milieu for Endothelial Differentiation of Human Adipose-Derived Stem Cells
Article Menu
Issue 4 (December) cover image

Export Article

Open AccessArticle
Bioengineering 2018, 5(4), 86; https://doi.org/10.3390/bioengineering5040086

A Standardized Collagen-Based Scaffold Improves Human Hepatocyte Shipment and Allows Metabolic Studies over 10 Days

1
Department of Traumatology, Siegfried Weller Institute, Eberhard Karls University, 72076 Tübingen, Germany
2
Matricel GmbH, 52134 Herzogenrath, Germany
3
Hepacult GmbH, 82152 Martinsried/Planegg, Germany
4
Biobank of the Department of General, Visceral and Transplantation Surgery, Hospital of the LMU, 81377 Munich, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 24 August 2018 / Revised: 12 October 2018 / Accepted: 14 October 2018 / Published: 16 October 2018
(This article belongs to the Special Issue Applying Polymeric Biomaterials in 3D Tissue Constructs)
Full-Text   |   PDF [2280 KB, uploaded 17 October 2018]   |  

Abstract

Due to pronounced species differences, hepatotoxicity of new drugs often cannot be detected in animal studies. Alternatively, human hepatocytes could be used, but there are some limitations. The cells are not always available on demand or in sufficient amounts, so far there has been only limited success to allow the transport of freshly isolated hepatocytes without massive loss of function or their cultivation for a long time. Since it is well accepted that the cultivation of hepatocytes in 3D is related to an improved function, we here tested the Optimaix-3D Scaffold from Matricel for the transport and cultivation of hepatocytes. After characterization of the scaffold, we shipped cells on the scaffold and/or cultivated them over 10 days. With the evaluation of hepatocyte functions such as urea production, albumin synthesis, and CYP activity, we showed that the metabolic activity of the cells on the scaffold remained nearly constant over the culture time whereas a significant decrease in metabolic activity occurred in 2D cultures. In addition, we demonstrated that significantly fewer cells were lost during transport. In summary, the collagen-based scaffold allows the transport and cultivation of hepatocytes without loss of function over 10 days. View Full-Text
Keywords: drug-induced hepatotoxicity; pre-clinical drug testing; cells shipment; natural collagen scaffolds drug-induced hepatotoxicity; pre-clinical drug testing; cells shipment; natural collagen scaffolds
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Ruoß, M.; Häussling, V.; Schügner, F.; Olde Damink, L.H.H.; Lee, S.M.L.; Ge, L.; Ehnert, S.; Nussler, A.K. A Standardized Collagen-Based Scaffold Improves Human Hepatocyte Shipment and Allows Metabolic Studies over 10 Days. Bioengineering 2018, 5, 86.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Bioengineering EISSN 2306-5354 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top