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Bioengineering 2018, 5(4), 82; https://doi.org/10.3390/bioengineering5040082

Milieu for Endothelial Differentiation of Human Adipose-Derived Stem Cells

Department of Biomedical Materials Science, School of Dentistry, University of Mississippi Medical Center, Jackson, MS 39216, USA
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Received: 30 August 2018 / Revised: 25 September 2018 / Accepted: 26 September 2018 / Published: 3 October 2018
(This article belongs to the Special Issue Applying Polymeric Biomaterials in 3D Tissue Constructs)
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Abstract

Human adipose-derived stem cells (hASCs) have been shown to differentiate down many lineages including endothelial lineage. We hypothesized that hASCs would more efficiently differentiate toward the endothelial lineage when formed as three-dimensional (3D) spheroids and with the addition of vascular endothelial growth factor (VEGF). Three conditions were tested: uncoated tissue culture polystyrene (TCPS) surfaces that induced a 2D monolayer formation; elastin-like polypeptide (ELP)-collagen composite hydrogel scaffolds that induced encapsulated 3D spheroid culture; and ELP-polyethyleneimine-coated TCPS surfaces that induced 3D spheroid formation in scaffold-free condition. Cells were exposed to endothelial differentiation medium containing no additional VEGF or 20 and 50 ng/mL of VEGF for 7 days and assayed for viability and endothelial differentiation markers. While endothelial differentiation media supported endothelial differentiation of hASCs, our 3D spheroid cultures augmented this differentiation and produced more von Willebrand factor than 2D cultures. Likewise, 3D cultures were able to uptake LDL, whereas the 2D cultures were not. Higher concentrations of VEGF further enhanced differentiation. Establishing angiogenesis is a key factor in regenerative medicine. Future studies aim to elucidate how to produce physiological changes such as neoangiogenesis and sprouting of vessels which may enhance the survival of regenerated tissues. View Full-Text
Keywords: Human adipose-derived stem cells; hydrogel; endothelial cells; scaffold-free culture Human adipose-derived stem cells; hydrogel; endothelial cells; scaffold-free culture
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Clark, K.; Janorkar, A.V. Milieu for Endothelial Differentiation of Human Adipose-Derived Stem Cells. Bioengineering 2018, 5, 82.

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