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Open AccessArticle

Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue

1
Advanced Ultrafast Laser Research Center and Brain Science Inspired Life Support Research Center, University of Electro-Communications, 1-5-1 Chofugaoka, Chofu, Tokyo 182-8585, Japan
2
JST, CREST, 5 Sanbancho, Chiyoda-ku, Tokyo 102-0075, Japan
3
Department of Physics, Tokyo University of Science, Kagurazaka 1-3, Shinjuku-ku, Tokyo 162-8601, Japan
4
Department of Electrophysics, National Chiao-Tung University, 1001 Ta Hsinchu Rd., Hsinchu 300, Taiwan
5
Research Center for Water Frontier Science and Technology, Tokyo University of Science, Kagurazaka 1-3, Shinjuku-ku, Tokyo 162-8601, Japan
6
Department of Occupational and Environmental Health, Graduate School of Medicine, Nagoya University, 65 Tsurumai-cho Showa-ku, Nagoya, Aichi 466-8550, Japan
7
Department of Pathology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan
*
Author to whom correspondence should be addressed.
Bioengineering 2018, 5(3), 67; https://doi.org/10.3390/bioengineering5030067
Received: 16 June 2018 / Revised: 10 August 2018 / Accepted: 12 August 2018 / Published: 15 August 2018
(This article belongs to the Special Issue Biomedical Photoacoustic and Photothermal Sensing and Imaging)
Label-free confocal photothermal (CPT) microscopy was utilized for the first time to investigate malignancy in mouse skin cells. Laser diodes (LDs) with 405 nm or 488 nm wavelengths were used as pumps, and a 638 nm LD was used as a probe for the CPT microscope. A Grey Level Cooccurrence Matrix (GLCM) for texture analysis was applied to the CPT images. Nine GLCM parameters were calculated with definite definitions for the intracellular super-resolved CPT images, and the parameters Entropy, Contrast, and Variance were found to be most suited among the nine parameters to discriminate clearly between healthy cells and malignant cells when a 405 nm pump was used. Prominence, Variance, and Shade were most suited when a pump wavelength of 488 nm was used. View Full-Text
Keywords: photothermal microscopy; label-free imaging; melanoma; texture analysis photothermal microscopy; label-free imaging; melanoma; texture analysis
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MDPI and ACS Style

Kobayashi, T.; Nakata, K.; Yajima, I.; Kato, M.; Tsurui, H. Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue. Bioengineering 2018, 5, 67.

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