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Open AccessArticle

A Precisely Flow-Controlled Microfluidic System for Enhanced Pre-Osteoblastic Cell Response for Bone Tissue Engineering

1
Department of Materials Science and Technology, University of Crete, Crete 70013, Greece
2
Foundation for Research and Technology-Hellas (FORTH), Institute of Electronic Structure and Laser (IESL), Crete 70013, Greece
*
Author to whom correspondence should be addressed.
Bioengineering 2018, 5(3), 66; https://doi.org/10.3390/bioengineering5030066
Received: 3 July 2018 / Revised: 3 August 2018 / Accepted: 9 August 2018 / Published: 12 August 2018
(This article belongs to the Special Issue Advanced Tissue Engineering Scaffolds)
Bone tissue engineering provides advanced solutions to overcome the limitations of currently used therapies for bone reconstruction. Dynamic culturing of cell-biomaterial constructs positively affects the cell proliferation and differentiation. In this study, we present a precisely flow-controlled microfluidic system employed for the investigation of bone-forming cell responses cultured on fibrous collagen matrices by applying two flow rates, 30 and 50 μL/min. We characterized the collagen substrates morphologically by means of scanning electron microscopy, investigated their viscoelastic properties, and evaluated the orientation, proliferation and osteogenic differentiation capacity of pre-osteoblastic cells cultured on them. The cells are oriented along the direction of the flow at both rates, in contrast to a random orientation observed under static culture conditions. The proliferation of cells after 7 days in culture was increased at both flow rates, with the flow rate of 50 μL/min indicating a significant increase compared to the static culture. The alkaline phosphatase activity after 7 days increased at both flow rates, with the rate of 30 μL/min indicating a significant enhancement compared to static conditions. Our results demonstrate that precisely flow-controlled microfluidic cell culture provides tunable control of the cell microenvironment that directs cellular activities involved in bone regeneration. View Full-Text
Keywords: microfluidics; collagen; osteogenic differentiation; cell orientation; MC3T3-E1 pre-osteoblasts microfluidics; collagen; osteogenic differentiation; cell orientation; MC3T3-E1 pre-osteoblasts
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MDPI and ACS Style

Babaliari, E.; Petekidis, G.; Chatzinikolaidou, M. A Precisely Flow-Controlled Microfluidic System for Enhanced Pre-Osteoblastic Cell Response for Bone Tissue Engineering. Bioengineering 2018, 5, 66.

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