Next Article in Journal
Assessment of Skeletal Tumor Load in Metastasized Castration-Resistant Prostate Cancer Patients: A Review of Available Methods and an Overview on Future Perspectives
Next Article in Special Issue
Advanced Dynamic Cell and Tissue Culture
Previous Article in Journal
Conceptual Design of Micro-Bioreactors and Organ-on-Chips for Studies of Cell Cultures
Previous Article in Special Issue
Gelatin-Methacryloyl (GelMA) Hydrogels with Defined Degree of Functionalization as a Versatile Toolkit for 3D Cell Culture and Extrusion Bioprinting
Article Menu
Issue 3 (September) cover image

Export Article

Open AccessReview
Bioengineering 2018, 5(3), 57; https://doi.org/10.3390/bioengineering5030057

Trinity of Three-Dimensional (3D) Scaffold, Vibration, and 3D Printing on Cell Culture Application: A Systematic Review and Indicating Future Direction

School of Engineering, University of South Australia; Mawson Lakes Blvd, Mawson Lakes 5095, Australia
*
Author to whom correspondence should be addressed.
Received: 31 May 2018 / Revised: 14 July 2018 / Accepted: 16 July 2018 / Published: 23 July 2018
(This article belongs to the Special Issue Advanced Dynamic Cell and Tissue Culture)
Full-Text   |   PDF [1375 KB, uploaded 24 July 2018]   |  

Abstract

Cell culture and cell scaffold engineering have previously developed in two directions. First can be ‘static into dynamic’, with proven effects that dynamic cultures have benefits over static ones. Researches in this direction have used several mechanical means, like external vibrators or shakers, to approximate the dynamic environments in real tissue, though such approaches could only partly address the issue. Second, can be ‘2D into 3D’, that is, artificially created three-dimensional (3D) passive (also called ‘static’) scaffolds have been utilized for 3D cell culture, helping external culturing conditions mimic real tissue 3D environments in a better way as compared with traditional two-dimensional (2D) culturing. In terms of the fabrication of 3D scaffolds, 3D printing (3DP) has witnessed its high popularity in recent years with ascending applicability, and this tendency might continue to grow along with the rapid development in scaffold engineering. In this review, we first introduce cell culturing, then focus 3D cell culture scaffold, vibration stimulation for dynamic culture, and 3DP technologies fabricating 3D scaffold. Potential interconnection of these realms will be analyzed, as well as the limitations of current 3D scaffold and vibration mechanisms. In the recommendation part, further discussion on future scaffold engineering regarding 3D vibratory scaffold will be addressed, indicating 3DP as a positive bridging technology for future scaffold with integrated and localized vibratory functions. View Full-Text
Keywords: cell culture; 3D scaffold; dynamicity and dimensionality; dynamic scaffold; 3D static or passive scaffold; future scaffold engineering; vibration; 3D printing (3DP), system evolution; 3D printed vibratory scaffold cell culture; 3D scaffold; dynamicity and dimensionality; dynamic scaffold; 3D static or passive scaffold; future scaffold engineering; vibration; 3D printing (3DP), system evolution; 3D printed vibratory scaffold
Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Yuan, H.; Xing, K.; Hsu, H.-Y. Trinity of Three-Dimensional (3D) Scaffold, Vibration, and 3D Printing on Cell Culture Application: A Systematic Review and Indicating Future Direction. Bioengineering 2018, 5, 57.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Bioengineering EISSN 2306-5354 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top