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Bioengineering 2017, 4(1), 4;

Iron Oxide Nanoparticles Stimulates Extra-Cellular Matrix Production in Cellular Spheroids

Department of Bioengineering, Clemson University, 301 Rhodes Research Center, Clemson, SC 29634, USA
Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina 173 Ashley Avenue—BSB 601, Charleston, SC 29425, USA
Institute of Biological Interfaces of Engineering, Department of Bioengineering, Clemson University, 401-2 Rhodes Engineering Research Center, Clemson, SC 29634, USA
Authors to whom correspondence should be addressed.
Academic Editor: Anthony Guiseppi-Elie
Received: 19 September 2016 / Revised: 12 December 2016 / Accepted: 22 December 2016 / Published: 21 January 2017
(This article belongs to the Special Issue Nanoparticle Interaction with the Biological Milieu)
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Nanotechnologies have been integrated into drug delivery, and non-invasive imaging applications, into nanostructured scaffolds for the manipulation of cells. The objective of this work was to determine how the physico-chemical properties of magnetic nanoparticles (MNPs) and their spatial distribution into cellular spheroids stimulated cells to produce an extracellular matrix (ECM). The MNP concentration (0.03 mg/mL, 0.1 mg/mL and 0.3 mg/mL), type (magnetoferritin), shape (nanorod—85 nm × 425 nm) and incorporation method were studied to determine each of their effects on the specific stimulation of four ECM proteins (collagen I, collagen IV, elastin and fibronectin) in primary rat aortic smooth muscle cell. Results demonstrated that as MNP concentration increased there was up to a 6.32-fold increase in collagen production over no MNP samples. Semi-quantitative Immunohistochemistry (IHC) results demonstrated that MNP type had the greatest influence on elastin production with a 56.28% positive area stain compared to controls and MNP shape favored elastin stimulation with a 50.19% positive area stain. Finally, there are no adverse effects of MNPs on cellular contractile ability. This study provides insight on the stimulation of ECM production in cells and tissues, which is important because it plays a critical role in regulating cellular functions. View Full-Text
Keywords: tissue engineering; spheroids; magnetic nanoparticles; extracellular matrix tissue engineering; spheroids; magnetic nanoparticles; extracellular matrix

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Casco, M.; Olsen, T.; Herbst, A.; Evans, G.; Rothermel, T.; Pruett, L.; Simionescu, D.; Visconti, R.; Alexis, F. Iron Oxide Nanoparticles Stimulates Extra-Cellular Matrix Production in Cellular Spheroids. Bioengineering 2017, 4, 4.

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