Utilizing Integrated Bioinformatics Analysis to Explore Potential Alterations in Mitochondrial Function Within Immune Cells Associated with Thoracic Aortic Aneurysms
Abstract
1. Introduction
2. Materials and Methods
2.1. Data Acquisition
2.2. Screening Differentially Expressed Mitochondrial-Related Genes (DE-MRGs) Between TAA and Control
2.3. snRNA-seq Data Processing
2.4. Evaluation of the Mitoscore
2.5. Construction of the LASSO Model and the SVM-RFE Feature Selection Process
2.6. ROC Curve Analysis
2.7. Animal Studies
2.8. qRT-PCR Analysis
2.9. Statistical Analysis
3. Results
3.1. Bulk RNA-seq Summarized the DE-MRGs in TAA
3.2. Mitochondrial Dysfunction of Immune Cells in TAA Based on snRNA-seq
3.3. Construction of a Diagnostic Model for TAA
3.4. Analysis of the Key DE-MRGs and Metabolic-Related Genes
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Symbol | ||
---|---|---|
LMNA | ARRB2 | ACADL |
MCL1 | SREBF1 | DCN |
PPP2R2B | MRPS6 | MAOA |
IL6 | PLN | IGF1 |
ALPL | TFRC | NAMPT |
FGR | MCUB |
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Guan, C.; Chen, S.-X.; Huang, C.-L.; Du, Y.-P.; Wang, K.-H.; Li, P.-X.; Liu, S.-R.; Liu, Z.-Y.; Huang, Z. Utilizing Integrated Bioinformatics Analysis to Explore Potential Alterations in Mitochondrial Function Within Immune Cells Associated with Thoracic Aortic Aneurysms. Bioengineering 2025, 12, 197. https://doi.org/10.3390/bioengineering12020197
Guan C, Chen S-X, Huang C-L, Du Y-P, Wang K-H, Li P-X, Liu S-R, Liu Z-Y, Huang Z. Utilizing Integrated Bioinformatics Analysis to Explore Potential Alterations in Mitochondrial Function Within Immune Cells Associated with Thoracic Aortic Aneurysms. Bioengineering. 2025; 12(2):197. https://doi.org/10.3390/bioengineering12020197
Chicago/Turabian StyleGuan, Chang, Si-Xu Chen, Chun-Ling Huang, Yi-Peng Du, Kai-Hao Wang, Pei-Xin Li, Shen-Rong Liu, Zhao-Yu Liu, and Zheng Huang. 2025. "Utilizing Integrated Bioinformatics Analysis to Explore Potential Alterations in Mitochondrial Function Within Immune Cells Associated with Thoracic Aortic Aneurysms" Bioengineering 12, no. 2: 197. https://doi.org/10.3390/bioengineering12020197
APA StyleGuan, C., Chen, S.-X., Huang, C.-L., Du, Y.-P., Wang, K.-H., Li, P.-X., Liu, S.-R., Liu, Z.-Y., & Huang, Z. (2025). Utilizing Integrated Bioinformatics Analysis to Explore Potential Alterations in Mitochondrial Function Within Immune Cells Associated with Thoracic Aortic Aneurysms. Bioengineering, 12(2), 197. https://doi.org/10.3390/bioengineering12020197