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Article

Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection

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Graduate School of Health Sciences, Showa University, Hatanodai 1-5-8, Shinagawa, Tokyo 142-8555, Japan
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Research Institute of Odontology (M-RIO), Meikai University, Keyakidai 1-1, Sakado, Saitama 350-0283, Japan
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Department of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Keyakidai 1-1, Sakado, Saitama 350-0295, Japan
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Department of Microbiology, St. Marianna University School of Medicine, Sugao 2-16-1, Miyamae, Kawasaki 216-8511, Japan
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Maruzen Pharmaceuticals Co., Ltd., Fukuyama, Hiroshima 729-3103, Japan
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Daiwa Biological Research Institute Co., Ltd., Sakado 3-2-1, Takatsu-ku, Kawasaki, Kanagawa 213-0012, Japan
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Division of Oral Health, School of Dentistry, Meikai University, Keyakidai 1-1, Sakado, Saitama 350-0283, Japan
*
Author to whom correspondence should be addressed.
Present Address: Laboratory of Microbiology, Showa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo 194-8543, Japan.
Medicines 2020, 7(10), 64; https://doi.org/10.3390/medicines7100064
Received: 11 August 2020 / Revised: 23 September 2020 / Accepted: 30 September 2020 / Published: 6 October 2020
Background: Herpes simplex virus (HSV) is usually dormant and becomes apparent when body conditions decline. We investigated the anti-HSV activity of various natural and synthetic compounds for future clinical application. Methods: Mock- and HSV-infected Vero cells were treated for three days with various concentrations of samples. For short exposure, 100-fold concentrated virus were preincubated for 3 min with samples, diluted to normal multiplicity of infection (MOI), before the addition to the cells. Anti-HSV activity was evaluated by the chemotherapy index. Results: Alkaline extracts of the leaves of Sasa sp. (SE) and pine cone (PCE) showed higher anti-HSV activity than 20 Japanese traditional herb medicines (Kampo formulas), four popular polyphenols, and 119 chromone-related compounds. Exposure of HSV to SE or PCE for 3 min almost completely eliminated the infectivity of HSV, whereas much longer exposure time was required for Kakkonto, the most active Kampo formulae. Anti-HSV activity of PCE and Kakkonto could be detected only when they were dissolved by alkaline solution (pH 8.0), but not by neutral buffer (pH 7.4). Anti-HSV activity of SE and povidone iodine was stable if they were diluted with neutral buffer. Conclusions: The present study suggests the applicability of SE and PCE for treatment of oral HSV and possibly other viruses. View Full-Text
Keywords: Kampo formulae; alkaline extract of Sasa sp.; pine cone extract; povidone-iodine; HSV; HIV; loss of infectivity; solubilization method Kampo formulae; alkaline extract of Sasa sp.; pine cone extract; povidone-iodine; HSV; HIV; loss of infectivity; solubilization method
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MDPI and ACS Style

Fukuchi, K.; Sakagami, H.; Sugita, Y.; Takao, K.; Asai, D.; Terakubo, S.; Takemura, H.; Ohno, H.; Horiuchi, M.; Suguro, M.; Fujisawa, T.; Toeda, K.; Oizumi, H.; Yasui, T.; Oizumi, T. Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection. Medicines 2020, 7, 64. https://doi.org/10.3390/medicines7100064

AMA Style

Fukuchi K, Sakagami H, Sugita Y, Takao K, Asai D, Terakubo S, Takemura H, Ohno H, Horiuchi M, Suguro M, Fujisawa T, Toeda K, Oizumi H, Yasui T, Oizumi T. Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection. Medicines. 2020; 7(10):64. https://doi.org/10.3390/medicines7100064

Chicago/Turabian Style

Fukuchi, Kunihiko, Hiroshi Sakagami, Yoshiaki Sugita, Koichi Takao, Daisuke Asai, Shigemi Terakubo, Hiromu Takemura, Hirokazu Ohno, Misaki Horiuchi, Madoka Suguro, Tomohiro Fujisawa, Kazuki Toeda, Hiroshi Oizumi, Toshikazu Yasui, and Takaaki Oizumi. 2020. "Quantification of the Ability of Natural Products to Prevent Herpes Virus Infection" Medicines 7, no. 10: 64. https://doi.org/10.3390/medicines7100064

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