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Open AccessArticle

In Vitro Anti-Cholinesterase and Antioxidant Activity of Extracts of Moringa oleifera Plants from Rivers State, Niger Delta, Nigeria

1
Department of Experimental Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Port Harcourt, Port Harcourt PMB 5323, Rivers State, Nigeria
2
Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt
3
Department of Pharmacology, Faculty of Basic Medical Sciences, University of Port Harcourt, Port Harcourt PMB 5323, Rivers State, Nigeria
4
School of Medicine, University of Nottingham, Royal Derby Hospital Centre, Derby DE22 3DT, UK
*
Author to whom correspondence should be addressed.
Medicines 2018, 5(3), 71; https://doi.org/10.3390/medicines5030071
Received: 15 May 2018 / Revised: 3 July 2018 / Accepted: 3 July 2018 / Published: 5 July 2018
(This article belongs to the Special Issue Anti-Neurodegenerative Agents from Natural Resources)
This study evaluated Moringa oleifera extracts from two locations in Niger Delta for in vitro anti-cholinesterase and antioxidant activities. Methanolic, aqueous and ethanolic extracts of Moringa oleifera were evaluated for inhibition of acetylcholinesterase (AChE) activity, antioxidant properties, and total phenolic and flavonoid contents using standard procedures. M. oleifera extracts possessed significant and concentration dependent AChE inhibitory activity for methanolic, aqueous, and ethanolic extracts. For the most potent extracts, the percentage AChE inhibition/IC50 (µg/mL) values were Moringa oleifera root methanolic extracts (MORME): ~80%/0.00845; Moringa oleifera root ethanolic extract 1 (MOREE1): ~90%/0.0563; Moringa oleifera root ethanolic extract 2 (MOREE2): ~70%/0.00175; and Moringa oleifera bark ethanolic extract (MOBEE): ~70%/0.0173. The descending order of AChE inhibitory potency of plant parts were: root > bark > leaf > flowers > seed. All M. oleifera methanolic extracts at a concentration of 1000 µg/mL displayed significant (p < 0.05–0.001) DPPH radical scavenging activity, with values of ~20–50% of that of ascorbic acid. The total phenolic content and total flavonoid content (TPC/TFC) of MORME, Moringa Oju bark methanolic extract (MOBME), MOREE1, MOREE2 and Moringa leaf ethanolic leaf extract (MLEE) were (287/254), (212/113), (223/185), (203/343) and (201/102) mg gallic acid equivalents/g and quercetin equivalents/g, respectively. There was an inverse correlation between plant extract AChE inhibition and total phenolic (p < 0.0001) and total flavonoid contents (p < 0.0012). In summary, this study revealed 5 of 19 extracts of M. oleifera that have potent in vitro anti-cholinesterase and antioxidant activities. View Full-Text
Keywords: Moringa oleifera; anti-cholinesterases; DPPH radical scavenging; antioxidant; oxidative stress; Alzheimer’s disease Moringa oleifera; anti-cholinesterases; DPPH radical scavenging; antioxidant; oxidative stress; Alzheimer’s disease
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MDPI and ACS Style

Nwidu, L.L.; Elmorsy, E.; Aprioku, J.S.; Siminialayi, I.; Carter, W.G. In Vitro Anti-Cholinesterase and Antioxidant Activity of Extracts of Moringa oleifera Plants from Rivers State, Niger Delta, Nigeria. Medicines 2018, 5, 71.

AMA Style

Nwidu LL, Elmorsy E, Aprioku JS, Siminialayi I, Carter WG. In Vitro Anti-Cholinesterase and Antioxidant Activity of Extracts of Moringa oleifera Plants from Rivers State, Niger Delta, Nigeria. Medicines. 2018; 5(3):71.

Chicago/Turabian Style

Nwidu, Lucky L.; Elmorsy, Ekramy; Aprioku, Jonah S.; Siminialayi, Iyeopu; Carter, Wayne G. 2018. "In Vitro Anti-Cholinesterase and Antioxidant Activity of Extracts of Moringa oleifera Plants from Rivers State, Niger Delta, Nigeria" Medicines 5, no. 3: 71.

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