Necrostatin-1 Attenuates Inflammatory Response and Improves Cognitive Function in Chronic Ischemic Stroke Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. Physiological Index and Mortality Rates in the Experimental Animals
2.2. Changes of Cerebral Blood Flow (CBF) in the Experimental Mice
2.3. Nec-1 Improves Cognitive Decline in the BCAS Mice
2.4. Nec-1 Decreased the Expression of Proteins from Hippocampus
2.5. Inflammatory Cytokine Protein and mRNA Levels were Reduced by Nec-1
2.6. Discussion
3. Experimental Section
3.1. Animals and Experimental Design
3.2. Establishment of Mouse Model of Bilateral Carotid Artery Stenosis Procedure (BCAS)
3.3. Drug Treatment
3.4. Water Maze Testing
3.5. Sample Collection
3.6. Western Blot Analysis
3.7. Enzyme-Linked Immunosorbent Assay Analysis (ELISA)
3.8. Real-Time PCR
3.9. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zhang, S.; Wang, Y.; Li, D.; Wu, J.; Si, W.; Wu, Y. Necrostatin-1 Attenuates Inflammatory Response and Improves Cognitive Function in Chronic Ischemic Stroke Mice. Medicines 2016, 3, 16. https://doi.org/10.3390/medicines3030016
Zhang S, Wang Y, Li D, Wu J, Si W, Wu Y. Necrostatin-1 Attenuates Inflammatory Response and Improves Cognitive Function in Chronic Ischemic Stroke Mice. Medicines. 2016; 3(3):16. https://doi.org/10.3390/medicines3030016
Chicago/Turabian StyleZhang, Shehong, Yuyang Wang, Dake Li, Junfa Wu, Wen Si, and Yi Wu. 2016. "Necrostatin-1 Attenuates Inflammatory Response and Improves Cognitive Function in Chronic Ischemic Stroke Mice" Medicines 3, no. 3: 16. https://doi.org/10.3390/medicines3030016