Next Article in Journal
Occurrence of Polychlorinated Dibenzo-p-Dioxins and Dibenzofurans and Polychlorinated Biphenyls in Fruit and Vegetables from the “Land of Fires” Area of Southern Italy
Previous Article in Journal
A Pilot Study in Cameroon to Understand Safe Uses of Pesticides in Agriculture, Risk Factors for Farmers’ Exposure and Management of Accidental Cases
Article

β-Cyclodextrin Attenuates Perfluorooctanoic Acid Toxicity in the Zebrafish Embryo Model

Department of Chemistry and Biochemistry, Florida International University, 11200 SW Eighth Street, Miami, FL 33199, USA
*
Authors to whom correspondence should be addressed.
Toxics 2017, 5(4), 31; https://doi.org/10.3390/toxics5040031
Received: 27 September 2017 / Revised: 31 October 2017 / Accepted: 1 November 2017 / Published: 4 November 2017
Perfluorooctanoic acid (PFOA) has been linked to negative health outcomes including cancer, thyroid disease, infertility, and developmental delays. β-Cyclodextrin (β-CD), a cyclic sugar, has been previously shown to form strong host–guest complexes with PFOA, and is proposed as a means of environmental remediation with respect to this widespread contaminant. In the present study, β-CD was directly examined with regards to possible attenuation of the toxicity of PFOA specifically employing the zebrafish (Danio rerio) embryo model. Zebrafish embryos were exposed to various concentrations of PFOA without β-CD, and with equimolar (1:1) and excess (2:1) molar ratios of β-CD to PFOA, and assessed for lethality and developmental toxicity through seven days post-fertilization (dpf). Rapid onset of lethality with limited morphological abnormalities was observed at relatively low concentrations of PFOA (LC50 ≈ 50 ppm), along with effects on morphometric and neurobehavioral parameters in surviving embryos. A highly significant difference (p < 0.0001) was observed between the 2:1 treatment, and both 1:1 and PFOA only treatments, with respect to lethal concentration and apparent neurobehavioral effects, suggesting an effectively reduced toxicity of the fully complexed PFOA. In contrast, however, neither β-CD treatment reduced developmental toxicity with respect to the morphometric endpoint (i.e., interocular distance). Whereas LC50 of PFOA alone did not change over 7 dpf, the 1:1 and 2:1 values decreased slightly over time, suggesting either delayed or alternative toxic effects on later developmental stages at presumptively lowered levels. This study, therefore, indicates β-CD may be an effective agent to reduce toxicity of and mitigate environmental health concerns associated with PFOA, but that further study is required to elucidate the mechanism of complexation as it relates to the attenuation of toxicity. View Full-Text
Keywords: perfluorooctanoic acid; β-Cyclodextrin; zebrafish embryo; host–guest chemistry; toxicity perfluorooctanoic acid; β-Cyclodextrin; zebrafish embryo; host–guest chemistry; toxicity
Show Figures

Figure 1

MDPI and ACS Style

Weiss-Errico, M.J.; Berry, J.P.; O’Shea, K.E. β-Cyclodextrin Attenuates Perfluorooctanoic Acid Toxicity in the Zebrafish Embryo Model. Toxics 2017, 5, 31. https://doi.org/10.3390/toxics5040031

AMA Style

Weiss-Errico MJ, Berry JP, O’Shea KE. β-Cyclodextrin Attenuates Perfluorooctanoic Acid Toxicity in the Zebrafish Embryo Model. Toxics. 2017; 5(4):31. https://doi.org/10.3390/toxics5040031

Chicago/Turabian Style

Weiss-Errico, Mary J., John P. Berry, and Kevin E. O’Shea 2017. "β-Cyclodextrin Attenuates Perfluorooctanoic Acid Toxicity in the Zebrafish Embryo Model" Toxics 5, no. 4: 31. https://doi.org/10.3390/toxics5040031

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop