Revelation of Different Nanoparticle-Uptake Behavior in Two Standard Cell Lines NIH/3T3 and A549 by Flow Cytometry and Time-Lapse Imaging
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Nanoparticles
2.3. FITC-Labeling of Titanium Dioxide Nanoparticles
2.4. Exposure to Cells with Titanium Dioxide Nanoparticles
2.5. Flow Cytometry and Sample Preparation
2.6. Time-Lapse and Fluorescence Microscopic Analysis
3. Results
3.1. Nanoparticle Labeling with FITC
3.2. Nanoparticle Uptake Measured by Side Scatter Signal
3.3. Forward Scattering of Investigated Cell Lines
3.4. Nanoparticle Uptake Measured by Fluorescence
3.5. Nanoparticle Uptake Kinetics Monitored by Microscopy
3.6. Interference with PI Signal
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Jochums, A.; Friehs, E.; Sambale, F.; Lavrentieva, A.; Bahnemann, D.; Scheper, T. Revelation of Different Nanoparticle-Uptake Behavior in Two Standard Cell Lines NIH/3T3 and A549 by Flow Cytometry and Time-Lapse Imaging. Toxics 2017, 5, 15. https://doi.org/10.3390/toxics5030015
Jochums A, Friehs E, Sambale F, Lavrentieva A, Bahnemann D, Scheper T. Revelation of Different Nanoparticle-Uptake Behavior in Two Standard Cell Lines NIH/3T3 and A549 by Flow Cytometry and Time-Lapse Imaging. Toxics. 2017; 5(3):15. https://doi.org/10.3390/toxics5030015
Chicago/Turabian StyleJochums, André, Elsa Friehs, Franziska Sambale, Antonina Lavrentieva, Detlef Bahnemann, and Thomas Scheper. 2017. "Revelation of Different Nanoparticle-Uptake Behavior in Two Standard Cell Lines NIH/3T3 and A549 by Flow Cytometry and Time-Lapse Imaging" Toxics 5, no. 3: 15. https://doi.org/10.3390/toxics5030015