Toxics 2016, 4(3), 22; https://doi.org/10.3390/toxics4030022
Differences in Reproductive Behavior between Spawning and Non-Spawning Zebrafish Pairs and the Effects of 17α-Ethinylestradiol (EE2)
Department of Bioscience, Zoophysiology, C.F. Moellers Alle 3, Aarhus University, building 1131, DK-8000 Aarhus C, Denmark
*
Author to whom correspondence should be addressed.
Academic Editor: Robert Tanguay
Received: 30 June 2016 / Revised: 21 August 2016 / Accepted: 29 August 2016 / Published: 6 September 2016
(This article belongs to the Special Issue Zebrafish as a Model for Assessing Chemical Toxicity)
Abstract
Reproductive success manifested by spawning and fertilization, in most fish, depends partly on an appropriate courtship behavior by both sexes. The zebrafish reproductive behavior can be resolved in some of its constituent elements by a computerized vision system and described in unbiased quantitative terms. Pairs of adult male and female zebrafish were monitored with automatic video tracking at 16 Hz for 45 min in a tank with a spawning area in one corner. Subsequently, spawning, if any, was registered and the swimming behavior and mutual interactions of the two fish were quantified. Further, temporal and frequency distributions of average velocity and turning rate were produced. It is demonstrated that the courtship behavior in spawning pairs differs markedly from non-spawning pairs with differences in both male and female behavior. EE2 (17α-ethinylestradiol), a contraceptive hormone found in aquatic environments, has only a slight effect on these behavior differences between spawning and non-spawning pairs. View Full-TextKeywords:
zebrafish; reproductive behavior; spawning; 17α-ethinylestradiol; EE2
▼
Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
Share & Cite This Article
MDPI and ACS Style
Henriksen, P.G.; Beedholm, K.; Baatrup, E. Differences in Reproductive Behavior between Spawning and Non-Spawning Zebrafish Pairs and the Effects of 17α-Ethinylestradiol (EE2). Toxics 2016, 4, 22.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.