Analytical Method Development and Dermal Absorption of 4-Amino-3-Nitrophenol (4A3NP), a Hair Dye Ingredient under the Oxidative or Non-Oxidative Condition
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. LC-MS/MS Analysis Conditions
2.3. Method Validation
2.4. In Vitro Dermal Absorption
2.5. Statistical Analysis
3. Results
3.1. Validation of Analytical Methodology (Accuracy and Precision)
3.2. In Vitro Dermal Absorption
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Name | MW | CAS No. | Chemical Composition | Log Pow | Structure | MS Transition (Positive) | ||
---|---|---|---|---|---|---|---|---|
Q1 | Q3 | CE | ||||||
4-amino-3-nitrophenol (4A3NP) | 154.12 | 610-81-1 | C6H6N2O3 | 0.41 | 154.9 | 137 | 15 | |
2-aminophenol (2AP) | 109.13 | 95-55-6 | C6H7NO | 0.62 | 109.9 | 92 | 23 |
Compound | Conc. (ng/mL) | Intraday (%) | Interday (%) | ||||
---|---|---|---|---|---|---|---|
Mean ± SD | Accuracy | Precision | Mean ± SD | Accuracy (%) | Precision (%) | ||
WASH (R2 = 0.9987) | 80 | 79.74 ± 1.77 | 99.68 | 2.23 | 79.89 ± 4.92 | 99.68 | 2.23 |
240 | 237.40 ± 4.04 | 98.92 | 1.70 | 238.60 ± 16.80 | 98.92 | 1.70 | |
400 | 413.20 ± 16.22 | 103.30 | 3.93 | 411.33 ± 36.16 | 103.30 | 3.93 | |
750 | 722.20 ± 54.27 | 96.29 | 7.51 | 734.2 ± 77.25 | 96.29 | 7.51 | |
SC (R2 = 0.9993) | 80 | 74.80 ± 5.19 | 93.50 | 6.94 | 77.83 ± 4.57 | 97.29 | 5.88 |
240 | 224.40 ± 7.67 | 93.50 | 3.42 | 240.86 ± 18.38 | 100.36 | 7.63 | |
400 | 389.80 ± 26.36 | 97.45 | 6.76 | 405.53 ± 23.78 | 101.38 | 5.86 | |
750 | 744.00 ± 31.01 | 99.20 | 4.17 | 780.13 ± 48.17 | 104.02 | 6.17 | |
SKIN (R2 = 0.9962) | 50 | 51.20 ± 2.18 | 102.40 | 4.25 | 50.08 ± 1.42 | 100.17 | 2.85 |
150 | 154.80 ± 11.56 | 103.20 | 7.47 | 151.06 ± 9.21 | 100.71 | 6.10 | |
400 | 445.40 ± 10.78 | 111.35 | 2.42 | 410.46 ± 27.57 | 102.62 | 6.72 | |
750 | 838.00 ± 20.17 | 111.73 | 2.41 | 752.80 ± 63.38 | 100.37 | 8.42 | |
RF (R2 = 0.9991) | 50 | 52.32 ± 7.56 | 104.64 | 14.46 | 50.19 ± 5.18 | 100.39 | 10.32 |
150 | 150.00 ± 5.10 | 100.00 | 3.40 | 153.26 ± 7.91 | 102.18 | 5.16 | |
400 | 407.60 ± 9.79 | 101.90 | 2.40 | 409.46 ± 7.54 | 102.37 | 1.84 | |
750 | 761.60 ± 24.93 | 101.55 | 3.27 | 752.60 ± 22.41 | 100.35 | 2.98 |
Substance | Conditions | Permeation Parameter Js (Equilibrium Flux, μg/cm2/h) |
---|---|---|
4A3NP | Oxidative (1%) | 0.16 ± 0.07 |
Non-oxidative (1.5%) | 0.23 ± 0.09 |
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Kim, H.Y.; Kim, Y.J.; Lee, J.D.; Kim, H.R.; Seo, D.-W. Analytical Method Development and Dermal Absorption of 4-Amino-3-Nitrophenol (4A3NP), a Hair Dye Ingredient under the Oxidative or Non-Oxidative Condition. Toxics 2024, 12, 340. https://doi.org/10.3390/toxics12050340
Kim HY, Kim YJ, Lee JD, Kim HR, Seo D-W. Analytical Method Development and Dermal Absorption of 4-Amino-3-Nitrophenol (4A3NP), a Hair Dye Ingredient under the Oxidative or Non-Oxidative Condition. Toxics. 2024; 12(5):340. https://doi.org/10.3390/toxics12050340
Chicago/Turabian StyleKim, Hyang Yeon, Yu Jin Kim, Jung Dae Lee, Hak Rim Kim, and Dong-Wan Seo. 2024. "Analytical Method Development and Dermal Absorption of 4-Amino-3-Nitrophenol (4A3NP), a Hair Dye Ingredient under the Oxidative or Non-Oxidative Condition" Toxics 12, no. 5: 340. https://doi.org/10.3390/toxics12050340
APA StyleKim, H. Y., Kim, Y. J., Lee, J. D., Kim, H. R., & Seo, D. -W. (2024). Analytical Method Development and Dermal Absorption of 4-Amino-3-Nitrophenol (4A3NP), a Hair Dye Ingredient under the Oxidative or Non-Oxidative Condition. Toxics, 12(5), 340. https://doi.org/10.3390/toxics12050340