Neurotoxicity of Combined Exposure to the Heavy Metals (Pb and As) in Zebrafish (Danio rerio)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Test Fish
2.2. Adult Zebrafish Culture
2.3. Histopathological Analysis
2.4. Swimming Behavior Detection
2.5. AChE Activity Assay
2.6. Determination of Neurotransmitter Levels
2.7. HPI Axis-Related Hormone Content Determination
2.8. Quantitative Real-Time PCR (qPCR) Assay
2.9. Statistical Analysis
3. Results
3.1. Histopathology Analysis
3.2. Swimming Performance Analysis
3.3. AChE Activity of Brains
3.4. DA and 5-HT Contents in Brains
3.5. HPI Axis Hormone Content
3.6. Alterations in Gene Transcript Levels
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, M.; Deng, P.; Li, G.; Liu, H.; Zuo, J.; Cui, W.; Zhang, H.; Chen, X.; Yao, J.; Peng, X.; et al. Neurotoxicity of Combined Exposure to the Heavy Metals (Pb and As) in Zebrafish (Danio rerio). Toxics 2024, 12, 282. https://doi.org/10.3390/toxics12040282
Liu M, Deng P, Li G, Liu H, Zuo J, Cui W, Zhang H, Chen X, Yao J, Peng X, et al. Neurotoxicity of Combined Exposure to the Heavy Metals (Pb and As) in Zebrafish (Danio rerio). Toxics. 2024; 12(4):282. https://doi.org/10.3390/toxics12040282
Chicago/Turabian StyleLiu, Ming, Ping Deng, Guangyu Li, Haoling Liu, Junli Zuo, Wenwen Cui, Huixian Zhang, Xin Chen, Jingjing Yao, Xitian Peng, and et al. 2024. "Neurotoxicity of Combined Exposure to the Heavy Metals (Pb and As) in Zebrafish (Danio rerio)" Toxics 12, no. 4: 282. https://doi.org/10.3390/toxics12040282