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Article

Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces

1
Food Quality and Safety Research Group, International Iberian Nanotechnology Laboratory, Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal
2
Department of Analytical Chemistry, Nutrition and Food Science, School of Veterinary Sciences, University of Santiago de Compostela, Campus de Lugo, 27002 Lugo, Spain
*
Author to whom correspondence should be addressed.
Foods 2020, 9(9), 1249; https://doi.org/10.3390/foods9091249
Received: 31 July 2020 / Revised: 31 August 2020 / Accepted: 2 September 2020 / Published: 7 September 2020
The continuous contamination of foods with L. monocytogenes, highlights the need for additional controls in the food industry. The verification of food processing plants is key to avoid cross-contaminations, and to assure the safety of the food products. In this study, a new methodology for the detection of L. monocytogenes on food contact surfaces was developed and evaluated. It combines Recombinase Polymerase Amplification (RPA) with the lateral flow (LF) naked-eye detection. Different approaches for the recovery of the bacteria from the surface, the enrichment step and downstream analysis by RPA-LF were tested and optimized. The results were compared with a standard culture-based technique and qPCR analysis. Sampling procedure with sponges was more efficient for the recovery of the bacteria than a regular swab. A 24 h enrichment in ONE broth was needed for the most sensitive detection of the pathogen. By RPA-LF, it was possible to detect 1.1 pg/µL of pure L. monocytogenes DNA, and the complete methodology reached a LoD50 of 4.2 CFU/cm2 and LoD95 of 18.2 CFU/cm2. These results are comparable with the culture-based methodology and qPCR. The developed approach allows for a next-day detection without complex equipment and a naked-eye visualization of the results. View Full-Text
Keywords: RPA; lateral flow; L. monocytogenes; surface analysis; food processing RPA; lateral flow; L. monocytogenes; surface analysis; food processing
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MDPI and ACS Style

Azinheiro, S.; Carvalho, J.; Prado, M.; Garrido-Maestu, A. Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces. Foods 2020, 9, 1249. https://doi.org/10.3390/foods9091249

AMA Style

Azinheiro S, Carvalho J, Prado M, Garrido-Maestu A. Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces. Foods. 2020; 9(9):1249. https://doi.org/10.3390/foods9091249

Chicago/Turabian Style

Azinheiro, Sarah, Joana Carvalho, Marta Prado, and Alejandro Garrido-Maestu. 2020. "Application of Recombinase Polymerase Amplification with Lateral Flow for a Naked-Eye Detection of Listeria monocytogenes on Food Processing Surfaces" Foods 9, no. 9: 1249. https://doi.org/10.3390/foods9091249

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