Preparation and Characterization of Soy Isoflavones Nanoparticles Using Polymerized Goat Milk Whey Protein as Wall Material
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Nanoparticle Preparation
2.2.1. Preparation of Goat Milk Whey Protein Concentrate
2.2.2. Preparation of Polymerized Goat Milk Whey Protein
2.2.3. Preparation of Soy Isoflavones Solution
2.2.4. PGWP-SIF Nanoparticle Preparation
2.3. Encapsulation Efficiency Determination
2.4. Particle Size and Zeta Potential Analysis
2.5. Rheological Properties Measurement
2.6. Fourier Transform Infrared (FT-IR) Spectroscopy
2.7. Fluorescence Spectroscopy
2.8. Differential Scanning Calorimetry (DSC)
2.9. Transmission Electron Microscopy (TEM)
2.10. Statistical Analysis
3. Results
3.1. Encapsulation Efficiency
3.2. Particle Size and Zeta Potential
3.3. Rheological Properties
3.4. FT-IR Spectra
3.5. Fluorescence Spectra
3.5.1. Inherent Fluorescence
3.5.2. Stern–Volmer Analysis of Quenching Data
3.5.3. Thermodynamic Parameters
3.5.4. Synchronous Fluorescence Spectra
3.6. Differential Scanning Calorimetry (DSC)
3.7. Microstructure
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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T (K) | Ksv (104 L/mol) | Kq (1012 L/mol) | R2 | Ka (105 L/mol) | n | R2 | ΔH (KJ/mol) | ΔG (KJ/mol) | ΔS (J/mol.K) |
---|---|---|---|---|---|---|---|---|---|
298 | 1.22 ± 0.1 | 1.22 ± 0.1 | 0.9988 | 7.80 ± 0.1 | 1.13 ± 0.02 | 0.9969 | 7.81 ± 0.1 | –33.74 ± 0.1 | 139.42 ± 1.2 |
303 | 1.20 ± 0.2 | 1.20 ± 0.2 | 0.9932 | 8.22 ± 0.1 | 0.93 ± 0.01 | 0.9873 | –34.43 ± 0.2 | ||
308 | 1.13 ± 0.2 | 1.13 ± 0.2 | 0.9927 | 8.34 ± 0.1 | 0.86 ± 0.02 | 0.9929 | –35.13 ± 0.2 |
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Tian, M.; Wang, C.; Cheng, J.; Wang, H.; Jiang, S.; Guo, M. Preparation and Characterization of Soy Isoflavones Nanoparticles Using Polymerized Goat Milk Whey Protein as Wall Material. Foods 2020, 9, 1198. https://doi.org/10.3390/foods9091198
Tian M, Wang C, Cheng J, Wang H, Jiang S, Guo M. Preparation and Characterization of Soy Isoflavones Nanoparticles Using Polymerized Goat Milk Whey Protein as Wall Material. Foods. 2020; 9(9):1198. https://doi.org/10.3390/foods9091198
Chicago/Turabian StyleTian, Mu, Cuina Wang, Jianjun Cheng, Hao Wang, Shilong Jiang, and Mingruo Guo. 2020. "Preparation and Characterization of Soy Isoflavones Nanoparticles Using Polymerized Goat Milk Whey Protein as Wall Material" Foods 9, no. 9: 1198. https://doi.org/10.3390/foods9091198