Next Article in Journal
Advances in Foodborne Pathogen Analysis
Next Article in Special Issue
Molecular Characterization of Cronobacter sakazakii Strains Isolated from Powdered Milk
Previous Article in Journal
Simultaneous Quantitative Assessment of Ochratoxin A, Patulin, 5-Hydroxymethylfurfural, and Bisphenol A in Fruit Drinks Using HPLC with Diode Array-Fluorimetric Detection
Previous Article in Special Issue
The Benefits of Whole Genome Sequencing for Foodborne Outbreak Investigation from the Perspective of a National Reference Laboratory in a Smaller Country
Article

The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology

1
ITQB NOVA, Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Av. da República, 2780-157 Oeiras, Portugal
2
iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2781-901 Oeiras, Portugal
3
Global Health and Tropical Medicine (GHTM) Research Center, Unidade de Microbiologia Médica, Instituto de Higiene e Medicina Tropical (IHTM), Universidade Nova de Lisboa (NOVA), 1349-008 Lisboa, Portugal
4
Laboratory of Viruses Contaminants of Water and Food, Genetics, Microbiology & Statistics Department, Universitat de Barcelona, 08028 Barcelona, Catalonia, Spain
5
The Water Research Institute (idRA), Universitat de Barcelona, 08001 Barcelona, Catalonia, Spain
*
Author to whom correspondence should be addressed.
Foods 2020, 9(11), 1634; https://doi.org/10.3390/foods9111634
Received: 20 October 2020 / Revised: 2 November 2020 / Accepted: 6 November 2020 / Published: 9 November 2020
Much of the knowledge on viruses is focused on those that can be propagated using cell-cultures or that can cause disease in humans or in economically important animals and plants. However, this only reflects a small portion of the virosphere. Therefore, in this study, we explore by targeted next-generation sequencing, how the virome varies between Atlantic horse mackerels and gilthead seabreams from fisheries and aquaculture from the center and south regions of Portugal. Viral genomes potentially pathogenic to fish and crustaceans, as well as to humans, were identified, namely Astroviridae, Nodaviridae, Hepadnaviridae, Birnaviridae, Caliciviridae, and Picornaviridae families. Also bacteriophages sequences were identified corresponding to the majority of sequences detected, with Myoviridae, Podoviridae, and Siphoviridae, the most widespread families in both fish species. However, these findings can also be due to the presence of bacteria in fish tissues, or even to contamination. Overall, seabreams harbored viruses from a smaller number of families in comparison with mackerels. Therefore, the obtained data show that fish sold for consumption can harbor a high diversity of viruses, many of which are unknown, reflecting the overall uncharacterized virome of fish. While cross-species transmission of bonafide fish viruses to humans is unlikely, the finding of human pathogenic viruses in fish suggest that fish virome can be a potential threat regarding food safety. View Full-Text
Keywords: virome diversity; metagenomics; Atlantic horse mackerel; gilthead seabream; health-threat virome diversity; metagenomics; Atlantic horse mackerel; gilthead seabream; health-threat
Show Figures

Figure 1

MDPI and ACS Style

Filipa-Silva, A.; Parreira, R.; Martínez-Puchol, S.; Bofill-Mas, S.; Barreto Crespo, M.T.; Nunes, M. The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology. Foods 2020, 9, 1634. https://doi.org/10.3390/foods9111634

AMA Style

Filipa-Silva A, Parreira R, Martínez-Puchol S, Bofill-Mas S, Barreto Crespo MT, Nunes M. The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology. Foods. 2020; 9(11):1634. https://doi.org/10.3390/foods9111634

Chicago/Turabian Style

Filipa-Silva, Andreia, Ricardo Parreira, Sandra Martínez-Puchol, Sílvia Bofill-Mas, Maria T. Barreto Crespo, and Mónica Nunes. 2020. "The Unexplored Virome of Two Atlantic Coast Fish: Contribution of Next-Generation Sequencing to Fish Virology" Foods 9, no. 11: 1634. https://doi.org/10.3390/foods9111634

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop