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Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci

University of Applied Sciences and Arts Hannover, Faculty II, Department Bioprocess Engineering, Microbiology, Heisterbergallee 10A, D-30453 Hannover, Germany
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Foods 2019, 8(2), 79; https://doi.org/10.3390/foods8020079
Received: 26 December 2018 / Revised: 25 January 2019 / Accepted: 18 February 2019 / Published: 20 February 2019
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Abstract

Mastitis poses a considerable threat to productivity and to animal welfare on modern dairy farms. However, the common way of antibiotic treatment does not always lead to a cure. Unsuccessful cures can, among other reasons, occur due to biofilm formation of the causative agent. This has attracted interest from researchers to introduce promising alternative therapeutic approaches, such as the use of beneficial lactic acid bacteria (LAB). In fact, using LAB for treating mastitis probably requires the formation of a beneficial biofilm by the probiotic bacteria. The present study investigated the ability of five LAB strains, selected on the basis of results from previous studies, to remove and to replace pathogenic biofilms in vitro. For this purpose, Staphylococcus (S.) aureus ATCC 12,600 and two strains—S. xylosus (35/07) and S. epidermidis (575/08)—belonging to the group of coagulase negative staphylococci (CNS) were allowed to form biofilms in a 96-well plate. Subsequently, the LAB were added to the well. The biofilm challenge was evaluated by scraping off and suspending the biofilm cells, followed by a plate count of serial dilutions using selective media. All the LAB strains successfully removed the staphylococcal biofilms. However, only Lactobacillus (L.) rhamnosus ATCC 7469 and L. plantarum 2/37 formed biofilms of their own to replace the pathogenic ones. View Full-Text
Keywords: lactic acid bacteria; biofilm; probiotic potential; staphylococci; mastitis lactic acid bacteria; biofilm; probiotic potential; staphylococci; mastitis
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Wallis, J.K.; Krömker, V.; Paduch, J.-H. Biofilm Challenge: Lactic Acid Bacteria Isolated from Bovine Udders versus Staphylococci. Foods 2019, 8, 79.

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