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Volume 14
Issue 19
10.3390/foods14193449
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Lacticaseibacillus paracasei subsp. paracasei 2LB: Identification of Genes to Assess the Safety and Probiotic Potential of the Strain

by
Gulyaim Abitayeva
*,
Diana Kurmangali
,
Temirlan Baikonys
and
Zhandarbek Bekshin
Republican Collection of Microorganisms LLP., Astana 010000, Kazakhstan
*
Author to whom correspondence should be addressed.
Foods 2025, 14(19), 3449; https://doi.org/10.3390/foods14193449
Submission received: 22 August 2025 / Revised: 1 October 2025 / Accepted: 2 October 2025 / Published: 9 October 2025
(This article belongs to the Section Food Microbiology)
Versions Notes

Abstract

In this study, we conducted a whole-genome analysis of the Lacticaseibacillus paracasei subsp. paracasei 2LB isolated from Kazakh traditional fermented milk (koumiss) to identify genes associated with the safety and probiotic potential of the strain. A comparative genomic analysis of the core and pan-genome of L. paracasei 2LB was performed. Functional annotation revealed the presence of genes putatively involved in metabolism, genetic information processing, and cellular processes. In terms of safety parameters, the stability of its genetic material, the absence of the ability to synthesize virulence factors, and genes responsible for antibiotic resistance were characterized. Also, in vitro studies of the L. paracasei 2LB strain showed resistance to stress factors and antimicrobial activity, and the presence of coding sequences encoding adhesion factors, bacteriocins, bile salts, pH, cold and heat shock, and osmotic stress was observed through genomic analysis. These results indicate that the L. paracasei 2LB strain is a potential probiotic candidate and demonstrate that whole-genome analysis is a useful method for assessing the quality and safety of probiotics.
Keywords: Lactocaseibacillus paracasei subsp. paracasei; whole genome sequencing; carbohydrate-active enzymes; safety and stability assessment; probiotic related multiple genes; probiotic potential; bacteriocins Lactocaseibacillus paracasei subsp. paracasei; whole genome sequencing; carbohydrate-active enzymes; safety and stability assessment; probiotic related multiple genes; probiotic potential; bacteriocins

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MDPI and ACS Style

Abitayeva, G.; Kurmangali, D.; Baikonys, T.; Bekshin, Z. Lacticaseibacillus paracasei subsp. paracasei 2LB: Identification of Genes to Assess the Safety and Probiotic Potential of the Strain. Foods 2025, 14, 3449. https://doi.org/10.3390/foods14193449

AMA Style

Abitayeva G, Kurmangali D, Baikonys T, Bekshin Z. Lacticaseibacillus paracasei subsp. paracasei 2LB: Identification of Genes to Assess the Safety and Probiotic Potential of the Strain. Foods. 2025; 14(19):3449. https://doi.org/10.3390/foods14193449

Chicago/Turabian Style

Abitayeva, Gulyaim, Diana Kurmangali, Temirlan Baikonys, and Zhandarbek Bekshin. 2025. "Lacticaseibacillus paracasei subsp. paracasei 2LB: Identification of Genes to Assess the Safety and Probiotic Potential of the Strain" Foods 14, no. 19: 3449. https://doi.org/10.3390/foods14193449

APA Style

Abitayeva, G., Kurmangali, D., Baikonys, T., & Bekshin, Z. (2025). Lacticaseibacillus paracasei subsp. paracasei 2LB: Identification of Genes to Assess the Safety and Probiotic Potential of the Strain. Foods, 14(19), 3449. https://doi.org/10.3390/foods14193449

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