Effects of Sequential Combination of Moderate Pressure and Ultrasound on Subsequent Thermal Pasteurization of Liquid Whole Egg
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Preparation
2.2. Samples Inoculation and Packaging
2.3. Moderate Pressure, Ultrasound and Thermal Pasteurization
2.4. Microbiological Analysis: Determination of Viable Cell Counts
2.5. pH and Color
2.6. Protein Solubility
2.7. Thermal Properties
2.8. Apparent Viscosity
2.9. Emulsifying Properties
2.10. Secondary Lipid Oxidation
2.11. Volatile Profile
2.12. Statistical Analysis
2.12.1. Analysis of Variance
2.12.2. Principal Component Analysis
3. Results and Discussion
3.1. Pre-Test Studies and Conditions Selection
3.2. Microbial Analyses
3.3. LWE Quality Properties Analyses
3.3.1. Physicochemical Properties
3.3.2. Thermal Properties
3.3.3. Emulsifying Properties
3.3.4. Secondary Lipid Oxidation
3.3.5. Volatile Profile
3.3.6. Principal Component Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatments | Salmonella Senftenberg 775/W | |
---|---|---|
MP | ||
1 | 50 MPa/5 min | 0.05 ± 0.06 a |
2 | 90 MPa/5 min | 0.11 ± 0.11 ab |
3 | 125 MPa/5 min | 0.16 ± 0.09 abc |
4 | 160 MPa/5 min | 0.67 ± 0.07 g |
MP–TP | ||
5 | 50 MPa/5 min–60 °C/1.75 min | 3.90 ± 0.17 j |
US | ||
6 | 50% amplitude/1 min | 0.26 ± 0.17 bcde |
7 | 50% amplitude/2 min | 0.25 ± 0.05 bcde |
8 | 50% amplitude/3 min | 0.38 ± 0.02 ef |
US–TP | ||
9 | 50% amplitude/1 min–60 °C/1.75 min | 3.54 ± 0.15 i |
10 | 50% amplitude/2 min–60 °C/1.75 min | 3.40 ± 0.05 i |
11 | 50% amplitude/3 min–60 °C/1.75 min | 4.15 ± 0.06 k |
US–MP | ||
12 | 50% amplitude/1 min–50 MPa/5 min | 0.31 ± 0.15 cde |
US–MP–TP | ||
13 | 50% amplitude/1 min–50 MPa/5 min–60 °C/1.75 min | 3.53 ± 0.15 i |
MP–US | ||
14 | 50 MPa/5 min–50% amplitude/1 min | 0.37 ± 0.14 def |
15 | 50 MPa/5 min–50% amplitude/2 min | 0.35 ± 0.03 def |
16 | 50 MPa/5 min–50% amplitude/3 min | 0.50 ± 0.02 fg |
17 | 90 MPa/5 min–50% amplitude/1 min | 0.20 ± 0.06 abcd |
18 | 125 MPa/5 min–50% amplitude/1 min | 0.30 ± 0.07 cde |
19 | 160 MPa/5 min–50% amplitude/1 min | 0.87 ± 0.08 h |
Properties | Raw | Thermal Pasteurization | Ultrasound | Moderate Pressure | Combined Process (MP–US–TP) 1 | |
---|---|---|---|---|---|---|
60 °C/3.5 min | 60 °C/1.75 min | 50%/1 min | 160 MPa/5 min | 160 MPa/5 min–50%/1 min–60 °C/1.75 min | ||
pH | 7.9 ± 0.0 a | 7.9 ± 0.0 ab | 7.9 ± 0.00 b | 7.8 ± 0.0 c | 7.8 ± 0.0 d | 7.8 ± 0.0 c |
L* | 44.0 ± 0.7 bc | 44.7 ± 0.12 a | 44.3 ± 0.7 ab | 44.7 ± 0.2 a | 44.1 ± 0.2 b | 43.5 ± 0.7 c |
a* | 9.8 ± 0.5 a | 10.0 ± 0.1 a | 9.7 ± 0.4 a | 9.9 ± 0.0 a | 10.0 ± 0.0 a | 9.8 ± 0.4 a |
b* | 11.1 ± 0.9 ab | 11.3 ± 0.3 ab | 11.0 ± 0.7 bc | 11.6 ± 0.1 a | 10.5 ± 0.2 c | 9.9 ± 0.7 d |
∆E | - | 0.5 ± 0.2 c | 0.4 ± 0.1 c | 1.0 ± 0.1 a | 0.8 ± 0.1 b | 0.8 ± 0.1 b |
Protein solubility (%) | 100.0 ± 3.4 b | 87.9 ± 2.9 d | 92.6 ± 3.2 c | 103.2 ± 2.6 ab | 90.8 ± 2.0 cd | 104.4 ± 3.9 a |
Viscosity (mPa.s, at shear rate of 50 s−1) | 13.0 ± 1.0 c | 20.6 ± 1.3 a | 20.5 ± 1.4 a | 8.6 ± 0.1 d | 18.3 ± 0.5 b | 9.1 ± 0.1 d |
Tonset (°C) | 75.9 ± 0.1 a | 77.9 ± 0.1 a | 77.6 ± 2.2 a | 78.3 ± 0.5 a | 80.0 ± 1.6 a | 77.8 ± 1.2 a |
Tpeak (°C) | 84.2 ± 0.6 b | 83.8 ± 1.0 b | 84.2 ± 0.1 b | 84.9 ± 0.1 b | 87.5 ± 0.1 a | 85.2 ± 0.4 b |
∆H (J/g) | 6.7 ± 0.8 a | 4.4 ± 0.4 ab | 4.7 ± 1.0 ab | 4.3 ± 0.3 ab | 3.94 ± 0.9 b | 6.1 ± 0.4 ab |
Emulsifying Activity Index (m2/g) | 65.8 ± 4.2 a | 44.2 ± 4.8 b | 40.8 ± 1.0 b | 23.4 ± 0.6 c | 67.1 ± 0.8 a | 24.8 ± 0.6 c |
Emulsifying Stability Index (min) | 1.3 ± 0.3 b | 0.6 ± 0.0 d | 1.0 ± 0.2 c | 0.6 ± 0.1 d | 1.7 ± 0.1 a | 0.7 ± 0.1 d |
Lipid Oxidation (TBARS, µg/100 g whole egg) | 13.3 ± 0.6 ac | 13.1 ± 0.5 a | 12.9 ± 0.6 a | 13.3 ± 0.4 ac | 10.7 ± 0.3 b | 13.8 ± 0.3 c |
Volatile Compounds | RT (min) | Liquid Whole Egg (µg/100 g Whole Egg) 1,2 | |||||
---|---|---|---|---|---|---|---|
Raw | 60 °C/3.5 min | 60 °C/1.75 min | 50%/1 min | 160 MPa/5 min | 160 MPa/5 min–50%/1 min–60 °C/1.75 min | ||
2-methyl pentane | 2.962 | 0.20 ± 0.06 abc | 0.18 ± 0.04 bc | 0.16 ± 0.02 c | 0.23 ± 0.09 abc | 0.32 ± 0.05 ab | 0.33 ± 0.04 a |
3-methyl pentane | 3.088 | 0.24 ± 0.02 b | 0.47 ± 0.06 a | 0.27 ± 0.01 b | 0.30 ± 0.06 b | 0.36 ± 0.04 ab | 0.32 ± 0.04 b |
Hexane | 3.242 | 9.52 ± 1.45 a | 9.38 ± 2.85 a | 12.32 ± 0.61 a | 2.90 ± 0.33 b | 2.72 ± 0.65 b | 2.52 ± 0.25 b |
Heptane | 5.002 | 18.50 ± 8.80 a | 24.26 ± 3.28 a | 22.82 ± 0.54 a | 2.13 ± 0.44 b | 4.24 ± 1.11 b | 12.91 ± 4.51 ab |
Decahydro-2-methylnaphthalene | 22.273 | 0.11 ± 0.01 b | 0.19 ± 0.05 a | 0.09 ± 0.02 b | 0.08 ± 0.00 b | 0.09 ± 0.02 b | 0.10 ± 0.02 b |
∑ Hydrocarbons | - | 35.51 ± 9.78 a | 41.06 ± 4.27 a | 42.63 ± 0.59 a | 8.42 ± 0.66 b | 9.83 ± 1.66 b | 19.47 ± 4.31 b |
Toluene | 7.498 | 133.22 ± 7.28 a | 125.31 ± 10.51 a | 113.08 ± 27.91 a | 29.71 ± 1.15 b | 3.60 ± 0.11 b | 4.33 ± 0.22 b |
∑ Total | - | 168.73 ± 8.89 a | 166.38 ± 6.34 a | 155.71 ± 28.34 a | 38.13 ± 1.53 b | 13.43 ± 1.77 b | 23.80 ± 4.50 b |
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Ribeiro, A.C.; Casal, S.; Lopes da Silva, J.A.; Saraiva, J.A. Effects of Sequential Combination of Moderate Pressure and Ultrasound on Subsequent Thermal Pasteurization of Liquid Whole Egg. Foods 2023, 12, 2459. https://doi.org/10.3390/foods12132459
Ribeiro AC, Casal S, Lopes da Silva JA, Saraiva JA. Effects of Sequential Combination of Moderate Pressure and Ultrasound on Subsequent Thermal Pasteurization of Liquid Whole Egg. Foods. 2023; 12(13):2459. https://doi.org/10.3390/foods12132459
Chicago/Turabian StyleRibeiro, Ana C., Susana Casal, José A. Lopes da Silva, and Jorge A. Saraiva. 2023. "Effects of Sequential Combination of Moderate Pressure and Ultrasound on Subsequent Thermal Pasteurization of Liquid Whole Egg" Foods 12, no. 13: 2459. https://doi.org/10.3390/foods12132459