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Volume 11
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10.3390/foods11060859
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Article

Using RSM for Optimum of Optimum Production of Peptides from Edible Bird’s Nest By-Product and Characterization of Its Antioxidant’s Properties

by
Jie Cao
1,
Ning Xiong
2,
Yu Zhang
1,
Yuwei Dai
1,
Yuye Wang
1,
Lingyu Lu
1 and
Lin Jiang
1,*
1
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China
2
School of Public Health, Southern Medical University, Guangzhou 510515, China
*
Author to whom correspondence should be addressed.
Foods 2022, 11(6), 859; https://doi.org/10.3390/foods11060859
Submission received: 16 February 2022 / Revised: 7 March 2022 / Accepted: 11 March 2022 / Published: 18 March 2022
(This article belongs to the Special Issue Current State-of-the-Art Technologies for Exploring Food-Derived Bioactive Peptides)
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Abstract

In this research, the neutrase hydrolysis conditions of edible bird’s nest (EBN) by-products were optimized by response surface methodology (RSM). Antioxidant peptides were then isolated from the EBN by-products by ultrafiltration and chromatography taking the DPPH radical scavenging ability as an indicator. The antioxidant activity of the purified peptides was estimated by radical scavenging ability and sodium nitroprusside (SNP)-induced damage model in PC12 cells. When the enzyme concentration was10 kU/g-hydrolysis temperature was 45 °C, and hydrolysis time was 10.30 h, the degree of hydrolysis (DH) of EBN by-product hydrolysate (EBNH) was the highest. The purified peptide exerted strong scavenging ability with EC50 values of 0.51, 1.31, and 0.65 mg/mL for DDPH, ABTS, and O2− radicals, respectively. In addition, the purified peptides could significantly reduce the SNP-induced oxidative damage of PC12 cells, and twelve peptides that were rich in leucine (Leu), valine (Val), and lysine (Lys) were identified by LC-MS/MS. These results suggested that EBN by-products have potential as new materials for natural antioxidant peptides.
Keywords: edible bird’s nest by-products; response surface methodology; antioxidant peptides; radical scavenging ability; PC12 cells edible bird’s nest by-products; response surface methodology; antioxidant peptides; radical scavenging ability; PC12 cells

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MDPI and ACS Style

Cao, J.; Xiong, N.; Zhang, Y.; Dai, Y.; Wang, Y.; Lu, L.; Jiang, L. Using RSM for Optimum of Optimum Production of Peptides from Edible Bird’s Nest By-Product and Characterization of Its Antioxidant’s Properties. Foods 2022, 11, 859. https://doi.org/10.3390/foods11060859

AMA Style

Cao J, Xiong N, Zhang Y, Dai Y, Wang Y, Lu L, Jiang L. Using RSM for Optimum of Optimum Production of Peptides from Edible Bird’s Nest By-Product and Characterization of Its Antioxidant’s Properties. Foods. 2022; 11(6):859. https://doi.org/10.3390/foods11060859

Chicago/Turabian Style

Cao, Jie, Ning Xiong, Yu Zhang, Yuwei Dai, Yuye Wang, Lingyu Lu, and Lin Jiang. 2022. "Using RSM for Optimum of Optimum Production of Peptides from Edible Bird’s Nest By-Product and Characterization of Its Antioxidant’s Properties" Foods 11, no. 6: 859. https://doi.org/10.3390/foods11060859

APA Style

Cao, J., Xiong, N., Zhang, Y., Dai, Y., Wang, Y., Lu, L., & Jiang, L. (2022). Using RSM for Optimum of Optimum Production of Peptides from Edible Bird’s Nest By-Product and Characterization of Its Antioxidant’s Properties. Foods, 11(6), 859. https://doi.org/10.3390/foods11060859

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