Effects of NaCl Concentration on the Behavior of Vibrio brasiliensis and Transcriptome Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Strains and Culture Conditions
2.2. Growth Conditions of V. brasiliensis in Salt Stress
2.3. Morphological Analysis of V. brasiliensis in Salt Stress
2.4. Swimming Motility of V. brasiliensis in Salt Stress
2.5. Extraction and Analysis of Outer Membrane Proteins (OMPs)
2.5.1. OMPs Extraction
2.5.2. Sodium Dodecyl Sulfate—Polyacrylamide Gel Electrophoresis (SDS-PAGE)
2.5.3. In Gel Tryptic Digest for Mass Spectrometry Analysis
2.6. Transcriptome Analysis
3. Results and Discussion
3.1. Effect of Salt Stress on Growth and Survival of V. brasiliensis
3.2. Morphological Analysis of V. brasiliensis in Response to Salt Stress
3.3. Effect of Salt Stress on the Swimming Ability of V. brasiliensis
3.4. Analysis of Differentially Expressed OMPs in Response to Salt Stress
3.5. Transcriptome Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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NaCl (%) | Accession | Gene | Proteins | Mw [KDa] | Molecular Function |
---|---|---|---|---|---|
2 | E8LNG0 | lpoA | Penicillin-binding protein activator LpoA | 67.3 | enzyme regulator activity |
2 | E8LPR8 | VIBR0546_18016 | Flp pilus assembly protein CpaB | 27.2 | NA |
2 | E8LQ55 | VIBR0546_05478 | MSHA biogenesis protein MshL | 59.2 | NA |
3 | E8LNG0 | lpoA | Penicillin-binding protein activator LpoA | 67.3 | enzyme regulator activity |
3 | E8LS16 | VIBR0546_10974 | Sodium-type flagellar protein MotY | 33.5 | NA |
5 | E8LS16 | VIBR0546_10974 | Sodium-type flagellar protein MotY | 33.5 | NA |
5 | E8M0I9 | VIBR0546_09489 | Outer membrane protein OmpA | 35.1 | porin activity |
7 | E8LS16 | VIBR0546_10974 | Sodium-type flagellar protein MotY | 33.5 | NA |
7 | E8M0I9 | VIBR0546_09489 | Outer membrane protein OmpA | 35.1 | porin activity |
7 | E8LX27 | VIBR0546_17898 | Ferric aerobactin receptor | 77.7 | siderophore uptake transmembrane transporter activity; signaling receptor activity |
Gene Name | Gene Description | FC | Log2FC | p Value | Regulate |
---|---|---|---|---|---|
VIBR0546_12042 | proton/glutamate symporter | 5.165 | 2.369 | 1.15 × 10−13 | up |
nhaA | pH-dependent sodium/proton antiporter | 3.672 | 1.877 | 1.61 × 10−12 | up |
VIBR0546_00894 | NptA protein | 11.824 | 3.564 | 5.86 × 10−12 | up |
VIBR0546_21825 | putative outer membrane protein | 7.040 | 2.816 | 6.8 × 10−11 | up |
VIBR0546_00270 | universal stress protein UspE | 2.950 | 1.561 | 6.62 × 10−8 | up |
VIBR0546_01129 | putative Na+/H+ antiporter | 3.182 | 1.670 | 1.85 × 10−7 | up |
VIBR0546_09929 | universal stress protein family 8 | 2.511 | 1.328 | 4.65 × 10−6 | up |
VIBR0546_10589 | universal stress protein A | 2.881 | 1.527 | 2.87 × 10−5 | up |
ompR | osmolarity response regulator | 2.471 | 1.305 | 9.71 × 10−5 | up |
VIBR0546_03992 | putative salt-induced outer membrane protein | 2.567 | 1.360 | 4.52 × 10−4 | up |
VIBR0546_17778 | OmpR family two-component response regulator | 2.039 | 1.028 | 9.87 × 10−3 | up |
VIBR0546_02830 | choline/carnitine/betaine transporter | 0.079 | −3.660 | 3.18 × 10−60 | down |
VIBR0546_00510 | outer membrane protein | 0.081 | −3.631 | 7.45 × 10−24 | down |
VIBR0546_06992 | sodium/proline symporter | 0.097 | −3.366 | 8.10 × 10−14 | down |
VIBR0546_03902 | putative sodium/sulfate symporter | 0.204 | −2.295 | 2.04 × 10−13 | down |
VIBR0546_13277 | Outer membrane protein | 0.263 | −1.926 | 5.11 × 10−12 | down |
VIBR0546_09484 | outer membrane protein OmpA | 0.250 | −2.000 | 4.01 × 10−11 | down |
VIBR0546_07217 | outer membrane protein W | 0.253 | −1.984 | 4.35 × 10−11 | down |
VIBR0546_05877 | OmpA family protein | 0.293 | −1.772 | 1.04 × 10−10 | down |
VIBR0546_18581 | sodium/alanine symporter | 0.383 | −1.385 | 8.86 × 10−9 | down |
VIBR0546_13282 | outer membrane protein | 0.396 | −1.338 | 1.58 × 10−6 | down |
VIBR0546_03420 | putative outer membrane protein | 0.370 | −1.434 | 2.10 × 10−5 | down |
VIBR0546_02985 | Outer membrane protein | 0.262 | −1.933 | 9.81 × 10−5 | down |
VIBR0546_16166 | putative arginine/ornithine antiporter | 0.478 | −1.066 | 2.62 × 10−3 | down |
Gene Name | Gene Description | FC | Log2FC | p Value | Regulate |
---|---|---|---|---|---|
VIBR0546_17048 | putative threonine efflux protein | 26.152 | 4.709 | 1.9 × 10−43 | up |
VIBR0546_21150 | Na+/H+ antiporter | 9.496 | 3.247 | 4.47 × 10−36 | up |
VIBR0546_01064 | Ca2+/Na+ antiporter | 10.100 | 3.336 | 5.15 × 10−32 | up |
VIBR0546_01411 | outer membrane protein | 4.147 | 2.052 | 1.00 × 10−30 | up |
VIBR0546_01241 | proton/glutamate symport protein | 4.243 | 2.085 | 2.23 × 10−17 | up |
VIBR0546_05214 | LPS assembly outer membrane complex protein LptD | 2.683 | 1.424 | 6.98 × 10−9 | up |
VIBR0546_21825 | putative outer membrane protein | 3.554 | 1.830 | 1.01 × 10−7 | up |
VIBR0546_10974 | sodium-type flagellar protein MotY precursor | 2.906 | 1.539 | 1.09 × 10−7 | up |
VIBR0546_21600 | putative sodium-type flagellar protein MotY precursor | 6.971 | 2.801 | 5.54 × 10−6 | up |
VIBR0546_03992 | putative salt-induced outer membrane protein | 2.468 | 1.304 | 3.57 × 10−5 | up |
VIBR0546_06697 | sodium/glutamate symporter | 2.705 | 1.435 | 1.36 × 10−4 | up |
VIBR0546_12742 | sodium/alanine symporter | 2.326 | 1.218 | 2.10 × 10−4 | up |
VIBR0546_12482 | putative outer membrane protein | 2.272 | 1.184 | 7.17 × 10−4 | up |
VIBR0546_07217 | outer membrane protein W | 0.008 | −6.889 | 9.60 × 10−120 | down |
VIBR0546_05309 | RNA polymerase sigma factor RpoS | 0.028 | −5.163 | 7.74 × 10−90 | down |
VIBR0546_00510 | outer membrane protein | 0.038 | −4.726 | 7.75 × 10−47 | down |
VIBR0546_20323 | outer membrane protein | 0.062 | −4.007 | 3.32 × 10−37 | down |
VIBR0546_00505 | putative anti-sigma regulatory factor | 0.030 | −5.066 | 5.08 × 10−36 | down |
VIBR0546_18581 | sodium/alanine symporter | 0.171 | −2.552 | 1.12 × 10−19 | down |
VIBR0546_09929 | universal stress protein family 8 | 0.160 | −2.647 | 3.93 × 10−19 | down |
VIBR0546_00270 | universal stress protein UspE | 0.221 | −2.179 | 3.5 × 10−15 | down |
VIBR0546_12042 | proton/glutamate symporter | 0.230 | −2.123 | 8.13 × 10−15 | down |
VIBR0546_09484 | outer membrane protein OmpA | 0.232 | −2.110 | 1.90 × 10−13 | down |
VIBR0546_10579 | universal stress protein UspB | 0.249 | −2.005 | 1.88 × 10−11 | down |
VIBR0546_06992 | sodium/proline symporter | 0.111 | −3.171 | 6.54 × 10−10 | down |
VIBR0546_10589 | universal stress protein A | 0.245 | −2.026 | 3.43 × 10−7 | down |
ompR | osmolarity response regulator | 0.365 | −1.452 | 5.85 × 10−7 | down |
VIBR0546_00894 | NptA protein | 0.231 | −2.116 | 1.03 × 10−4 | down |
VIBR0546_05877 | OmpA family protein | 0.427 | −1.229 | 1.20 × 10−4 | down |
VIBR0546_05598 | outer membrane protein OmpK | 0.420 | −1.252 | 1.06 × 10−3 | down |
VIBR0546_01821 | Outer membrane protein | 0.217 | −2.202 | 2.26 × 10−3 | down |
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Hu, S.; Li, Y.; Wang, B.; Yin, L.; Jia, X. Effects of NaCl Concentration on the Behavior of Vibrio brasiliensis and Transcriptome Analysis. Foods 2022, 11, 840. https://doi.org/10.3390/foods11060840
Hu S, Li Y, Wang B, Yin L, Jia X. Effects of NaCl Concentration on the Behavior of Vibrio brasiliensis and Transcriptome Analysis. Foods. 2022; 11(6):840. https://doi.org/10.3390/foods11060840
Chicago/Turabian StyleHu, Shuyang, Yuwei Li, Boran Wang, Lijun Yin, and Xin Jia. 2022. "Effects of NaCl Concentration on the Behavior of Vibrio brasiliensis and Transcriptome Analysis" Foods 11, no. 6: 840. https://doi.org/10.3390/foods11060840