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Article

Prevalence, Antibiogram and Genetic Characterization of Listeria monocytogenes from Food Products in Egypt

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Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Egypt
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Department of Animal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Egypt
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Faculty of Veterinary Medicine, Veterinarian Teaching Hospital, University of Sadat City, Sadat City 32897, Egypt
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Animal Health Research Institute (AHRI)—Dokki, Giza 12618, Egypt
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Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh
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Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
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Center of Biomedical Sciences Research (CBSR), Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
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Department of Clinical Laboratories Sciences, The Faculty of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
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Center of Excellence for Screening of Environmental Contaminants, Benha University, Toukh 13736, Egypt
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Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Toukh 13736, Egypt
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Author to whom correspondence should be addressed.
Academic Editors: Fernando Pérez-Rodriguez and Araceli Bolívar
Foods 2021, 10(6), 1381; https://doi.org/10.3390/foods10061381
Received: 3 June 2021 / Revised: 6 June 2021 / Accepted: 9 June 2021 / Published: 15 June 2021
(This article belongs to the Special Issue Listeria in Food: Prevalence and Control)
World Health Organization classified Listeria monocytogenes as a major notable foodborne pathogen associated with high mortality and hospitalization. The study reports the prevalence, antibiogram, virulence determination and genetic characterization of L. monocytogenes from different food products. A total of 250 food samples, fifty samples each from raw milk, ice cream, minced meat, fish fillet and sausage were collected from the Menoufiya governorate in Egypt. L. monocytogenes was detected in 17 (6.8%) of the tested food samples including minced meat (14%), fish fillet (8%), sausage (6%) and raw milk (6%). The antimicrobial susceptibility assay of 17 L. monocytogenes isolates against seventeen antibiotics belonging to eight antibiotics classes revealed a high susceptibility to norfloxacin (82.3%), amoxicillin-clavulanic acid (76.4%), cefotaxime (70.5%), erythromycin (64.6%), amoxicillin (64.6%), gentamicin (58.7%) and vancomycin (58.7%). While, high resistance was observed against oxytetracycline (76.4%), trimethoprim-sulfamethoxazole (76.4%), chloramphenicol (70.5%), doxycycline (64.6%), levofloxacin (41.2%) and azithromycin (41.2%). Of note, all L. monocytogenes isolates were multidrug-resistant. The multiplex PCR successfully amplified L. monocytogenes in all tested isolates. Screening of the five virulence-related genes revealed the hlyA and iap as the most prevalent genes followed by actA gene, however, the inlA and prfA genes were not detected in any of the studied isolates. The partial 16S rRNA gene sequencing of three L. monocytogenes isolates showed a high nucleotide similarity (99.1–99.8%) between the study isolates and various global clones, and phylogenetic analysis clustered these L. monocytogenes strains with other Listeria species including L. welshimeri, L. seeligeri and L. innocua. This study demonstrates the impact of L. monocytogenes as a major contaminant of various food products and suggests more attention to the awareness and hygienic measures in the food industry. View Full-Text
Keywords: L. monocytogenes; virulence genes; foods; sequencing; antibiogram L. monocytogenes; virulence genes; foods; sequencing; antibiogram
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MDPI and ACS Style

Abdeen, E.E.; Mousa, W.S.; Harb, O.H.; Fath-Elbab, G.A.; Nooruzzaman, M.; Gaber, A.; Alsanie, W.F.; Abdeen, A. Prevalence, Antibiogram and Genetic Characterization of Listeria monocytogenes from Food Products in Egypt. Foods 2021, 10, 1381. https://doi.org/10.3390/foods10061381

AMA Style

Abdeen EE, Mousa WS, Harb OH, Fath-Elbab GA, Nooruzzaman M, Gaber A, Alsanie WF, Abdeen A. Prevalence, Antibiogram and Genetic Characterization of Listeria monocytogenes from Food Products in Egypt. Foods. 2021; 10(6):1381. https://doi.org/10.3390/foods10061381

Chicago/Turabian Style

Abdeen, Eman E., Walid S. Mousa, Ola. H. Harb, Gehad A. Fath-Elbab, Mohammed Nooruzzaman, Ahmed Gaber, Walaa F. Alsanie, and Ahmed Abdeen. 2021. "Prevalence, Antibiogram and Genetic Characterization of Listeria monocytogenes from Food Products in Egypt" Foods 10, no. 6: 1381. https://doi.org/10.3390/foods10061381

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