Effect of High Hydrostatic Pressure on the Extractability and Bioaccessibility of Carotenoids and Their Esters from Papaya (Carica papaya L.) and Its Impact on Tissue Microstructure
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Chemicals, Standards and Reagents
2.3. High Hydrostatic Pressure Treatments
2.4. In Vitro Gastrointestinal Digestion Assay
2.5. Carotenoid Analysis
2.5.1. Carotenoid Extraction from Fresh and Freeze-Dried Papaya
2.5.2. Carotenoid Extraction from In Vitro Digestion Phases
2.6. Carotenoid Analysis by HPLC-DAD
2.7. Liquid Chromatography Mass Spectrometry (LC-MS/MS (APCI+))
2.8. Optical Microscopy
2.9. Statistical Analysis
3. Results and Discussion
3.1. Characterization of Carotenoid and Carotenoid Ester Profile
3.1.1. Individual Carotenoid Profile in Papaya Tissues
3.1.2. Individual Carotenoid and Carotenoid Ester Content in Papaya Pulp Processed by HHP
3.2. Stability of Carotenoids and Carotenoid Esters in Papaya during In Vitro Digestion
3.3. Bioccessibility of Carotenoid and Carotenoid Esters in Papaya Submitted to HHP Treatments
3.4. Microstructure of Papaya Fruit and Digesta during In Vitro Digestion
3.4.1. Effect of HHP on Cell Wall and Morphology of Papaya Pulp
3.4.2. Carotenoid Deposition and Factors Affecting Their Stability during In Vitro Digestion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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cv. Sweet Mary | ||||||||
---|---|---|---|---|---|---|---|---|
CUT | 5 min | |||||||
No | Carotenoid Compound | Control | 100 MPa | 350 MPa | 600 MPa | 100 MPa | 350 MPa | 600 MPa |
1 | (13Z)-violaxanthin | 1 ± 0 a | 64 ± 4 b | 36 ± 2 ab | 66 ± 2 b | 6 ± 0 a | 164 ± 29 c | 17 ± 1 a |
2 | (all-E)-violaxanthin | 3 ± 0 a | 85 ± 2 d | 65 ± 0 c | 50 ± 2 b | 15 ± 1 a | 122 ± 8 e | 13 ± 2 a |
3 | (9Z)-neoxanthin | n.d. a | 33 ± 1 c | 52 ± 3 d | 63 ± 0 e | 17 ± 1 b | 74 ± 1 f | 15 ± 0 b |
4 | (all-E)-neoxanthin | n.d. a | 23 ± 0 b | n.d. a | 18 ± 0 b | 14 ± 0 b | 87 ± 9 c | n.d. a |
5 | (all-E)-lutein | n.d. a | 50 ± 3 b | n.d. a | 42 ± 3 b | n.d. a | 270 ± 11 c | n.d. a |
6 | (all-E)-zeaxanthin | n.d. a | 33 ± 1 b | n.d. a | 97 ± 3 c | 28 ± 0 b | 225 ± 2 d | n.d. a |
7 | Lutein-5,6-epoxide | n.d. a | 87 ± 3 c | n.d. a | 47 ± 3 b | n.d. a | 143 ± 1 d | n.d. a |
8 | (all-E)-antheraxanthin | 15 ± 1 b | 38 ± 0 c | n.d. a | 15 ± 1 b | n.d. a | 77 ± 2 d | n.d. a |
9 | (9Z)-violaxanthin | 6 ± 0 b | 50 ± 3 c | n.d. a | 11 ± 1 b | 7 ± 1 b | 49 ± 2 c | n.d. a |
10 | β-cryptoxanthin-5,6-epoxide | n.d. a | 46 ± 1 c | n.d. a | 17 ± 1 b | n.d. a | n.d. a | n.d. a |
11 | (9Z)-α-cryptoxanthin | n.d. a | 25 ± 1 b | n.d. a | 35 ± 0 c | n.d. a | n.d. a | n.d. a |
12 | (all-E)-α-cryptoxanthin | 3 ± 0 c | 4 ± 0 c | n.d. a | 6 ± 1 d | 2 ± 0 b | n.d. a | n.d. a |
13 | (all-E)-β-cryptoxanthin | 43 ± 1 a | 388 ± 3 e | 92 ± 3 c | 305 ± 0 d | 79 ± 1 b | 73 ± 2 b | 29 ± 2 a |
14 | α-carotene-5,6-epoxide | 3 ± 0 b | 24 ± 0 c | n.d. a | n.d. a | 21 ± 1 c | 40 ± 0 d | n.d. a |
15 | (all-E)-luteoxanthin | n.d. a | 39 ± 0 b | n.d. a | n.d. a | n.d. a | n.d. a | n.d. a |
16 | (13Z)-α-carotene | 14 ± 0 c | 14 ± 0 c | n.d. a | 22 ± 1 f | 19 ± 1 d | 77 ± 1 e | 5 ± 0 b |
17 | (13Z)-β-carotene | 4 ± 0 b | 41 ± 0 e | 32 ± 0 d | n.d. a | 35 ± 1 d | 10 ± 1 b | n.d. a |
18 | (all-E)-violaxanthin laurate | 11 ± 1 b | 10 ± 0 b | n.d. a | 21 ± 1 c | 25 ± 1 c | 34 ± 0 d | 41 ± 3 e |
19 | α-cryptoxanthin-5,8-epoxide | 8 ± 1 d | 8 ± 0 d | 7 ± 0 c | n.d. a | 6 ± 0 b | 11 ± 0 e | 5 ± 1 b |
20 | (all-E)-ζ-carotene | 4 ± 0 b | 13 ± 0 c | n.d. a | 21 ± 1 d | 33 ± 2 e | 18 ± 1 d | 12 ± 1 c |
21 | α-cryptoxanthin-5,8′-epoxide | 29 ± 0 b | 13 ± 0 a | 12 ± 0 a | 15 ± 1 a | 30 ± 2 b | 13 ± 1 a | 15 ± 1 a |
22 | (all-E)-α-carotene | 75 ± 0 c | 77 ± 0 c | 81 ± 0 c | 59 ± 4 b | 76 ± 5 c | 85 ± 0 c | 40 ± 2 a |
23 | (9Z)-α-carotene | 3 ± 0 b | n.d. a | n.d. a | n.d. a | n.d. a | 8 ± 0 c | n.d. a |
24 | (9Z)-violaxanthin laurate | 51 ± 3 e | 16 ± 1 c | n.d. a | 23 ± 1 d | 103 ± 2 g | 11 ± 1 b | 92 ± 3 f |
25 | (all-E)-lutein-3-O-myristate | 169 ± 0 b | 31 ± 1 a | n.d. a | 144 ± 1 b | 247 ± 13 c | 218 ± 19 c | 165 ± 16 b |
26 | (all-E)-β-carotene | 165 ± 7 b | 203 ± 12 b | 649 ± 17 f | 250 ± 13 c | 315 ± 2 d | 359 ± 2 e | 140 ± 7 a |
27 | (9Z)-β-carotene | 6 ± 0 a | n.d. a | 49 ± 4 d | 20 ± 1 b | n.d. a | 33 ± 1 c | 7 ± 1 a |
28 | (all-E)-violaxanthin dimyristate | 36 ± 7 b | n.d. a | 69 ± 5 d | 46 ± 1 c | n.d. a | 54 ± 1 c | 48 ± 3 c |
29 | (all-E)-antheraxanthin myristate palmitate | 43 ± 2 b | 33 ± 1 a | 126 ± 3 f | 69 ± 1 c | 27 ± 0 a | 94 ± 1 e | 81 ± 5 d |
30 | (all-E)-violaxanthin palmitate | 7 ± 1 b | 13 ± 0 c | 47 ± 1 e | 23 ± 0 d | n.d. a | 27 ± 1 d | 16 ± 2 c |
31 | (9Z)-neoxanthin dibutyrate | 8 ± 0 b | n.d. a | 43 ± 1 e | 18 ± 0 c | 81 ± 2 f | 26 ± 0 d | 2 ± 2 b |
32 | (all-E)-β-cryptoxanthin caprate | 82 ± 2 b | 32 ± 1 a | 236 ± 11 f | 111 ± 3 c | 135 ± 0 d | 170 ± 5 e | 72 ± 5 b |
33 | (all-E)-violaxanthin myristate palmitate | n.d. a | n.d. a | 14 ± 1 b | n.d. a | n.d. a | n.d. a | n.d. a |
34 | (all-E)-lutein dimyristate | 60 ± 4 c | 7 ± 0 a | 162 ± 3 f | 40 ± 3 b | 110 ± 0 e | 65 ± 4 c | 87 ± 4 d |
35 | (all-E)-β-cryptoxanthin laurate | 175 ± 10 b | 59 ± 1 a | 365 ± 13 e | 212 ± 2 c | 209 ± 0 c | 338 ± 9 d | 146 ± 9 b |
36 | (all-E)-antheraxanthin-3-O palmitate | n.d. a | n.d. a | 86 ± 4 c | n.d. a | n.d. a | 54 ± 0 b | n.d. a |
37 | (all-E)-antheraxanthin laurate myristate | 22 ± 2 b | 12 ± 0 a | 28 ± 1 c | 33 ± 2 d | 33 ± 1 d | 18 ± 1 b | 21 ± 2 b |
38 | (all-E)-β-cryptoxanthin myristate | 19 ± 1 b | 5 ± 0 a | 62 ± 0 f | 25 ± 1 d | 22 ± 0 c | 42 ± 1 e | 16 ± 1 b |
39 | (Z)-lycopene isomer 1 | 14 ± 0 b | n.d. a | 23 ± 1 d | n.d. a | 6 ± 0 b | 20 ± 1 c | n.d. a |
40 | (all-E)-β-cryptoxanthin palmitate | 14 ± 0 c | n.d. a | 25 ± 2 e | 5 ± 1 bc | 7 ± 0 c | 16 ± 0 d | 3 ± 0 ab |
41 | (13Z)-lycopene isomer 2 | 113 ± 0 f | 78 ± 2 d | 63 ± 1 c | 47 ± 2 b | 108 ± 0 e | 114 ± 3 f | 40 ± 2 a |
42 | (13′Z)-lycopene isomer 3 | 22 ± 0 d | 16 ± 0 c | 12 ± 0 b | n.d. a | 21 ± 1 d | 21 ± 0 d | n.d. a |
43 | (9Z)-lycopene isomer 4 | 26 ± 0 d | 9 ± 0 b | 15 ± 0 c | n.d. a | 13 ± 0 bc | 9 ± 0 b | 32 ± 3 d |
44 | (9′Z)-lycopene isomer 5 | 12 ± 0 c | 7 ± 0 b | 19 ± 1 d | n.d. a | 10 ± 0 c | n.d. a | n.d. a |
45 | (all-E)-lycopene | 378 ± 5 c | 120 ± 4 a | 374 ± 15 c | 359 ± 15 c | 231 ± 4 b | 1302 ± 52 d | 316 ± 19 c |
46 | (Z)-lycopene isomer 6 | 23 ± 1 b | n.d. a | n.d. a | n.d. a | n.d. a | n.d. a | n.d. a |
Total free xanthophylls | 109 ± 4 a | 985 ± 4 d | 264 ± 9 b | 787 ± 7 c | 204 ± 3 b | 1308 ± 66 e | 95 ± 6 a | |
Total hydrocarbon carotenoids | 696 ± 30 a | 602 ± 11 a | 1314 ± 37 c | 778 ± 29 b | 888 ± 1 b | 2096 ± 52 d | 590 ± 37 a | |
Total xanthophyll esters | 859 ± 15 b | 219 ± 4 a | 1263 ± 43 e | 770 ± 3 b | 997 ± 18 c | 1166 ± 5 d | 811 ± 54 b | |
Total carotenoids | 1664 ± 48 a | 1807 ± 11 b | 2841 ± 89 d | 2336 ± 33 c | 2089 ± 14 b | 4469 ± 124 e | 1496 ± 99 a | |
RAE | 23 ± 1 a | 44 ± 1 b | 93 ± 3 e | 52 ± 1 c | 51 ± 0 c | 67 ± 1 d | 25 ± 2 a |
Compound | Phase (Digesta) | Non-Treated | 100 MPa/CUT | 100 MPa/5 min | 350 MPa/CUT | 350 MPa/5 min | 600 MPa/CUT | 600 MPa/5 min |
---|---|---|---|---|---|---|---|---|
Free xanthophylls | ||||||||
(all-E)-violaxanthin | Oral | 27.4 ± 1.8 Ab | 61.7 ± 1.1 Af | 65.5 ± 0.1 Af | 22.2 ± 0.2 Aa | 39.3 ± 0.8 Ac | 57.1 ± 0.8 Ae | 46.4 ± 0.8 Ad |
Gastric | 175.4 ±1.8 Bf | 73.0 ± 0.3 Bc | 149.1 ± 1.7 Ce | 39.6 ± 0.4 Ba | 181.9 ± 0.8 Cg | 66.4 ± 0.3 Bb | 91.6 ± 2.6 Cd | |
Intestinal | 150.8 ± 3.9 Be | 110.9 ± 0.1 Cd | 114.8 ± 0.1 Bd | 63.4 ± 1.3 Ca | 91.0 ± 2.3 Bc | 81.5 ± 0.7 Cb | 61.0 ± 0.4 Ba | |
(all-E)-zeaxanthin | Oral | 15.8 ± 0.9 Aa | 33.1 ± 0.4 Ab | 39.6 ± 0.7 Bc | 51.4 ± 0.2 Ce | 43.1 ± 1.0 Ad | 51.0 ± 0.4 Be | 54.9 ± 0.7 Bf |
Gastric | 65.1 ± 3.2 Ce | 71.0 ± 0.4 Cf | 43.3 ± 0.9 Cd | 34.4 ± 0.1 Bb | 51.1 ± 0.1 Bd | 19.2 ± 0.6 Aa | 44.9 ± 0.5 Ab | |
Intestinal | 47.6 ± 1.6 Bb | 59.3 ± 0.2 Bd | 22.0 ± 0.1 Aa | 22.3 ± 0.3 Aa | 93.3 ± 0.5 Cf | 89.3± 1.4 Ce | 54.1 ± 0.7 Bc | |
(all-E)-antheraxanthin | Oral | 210.7± 0.4 Cc | 137.6 ± 0.4 Ab | n.d. Aa | n.d. Aa | n.d. Aa | n.d. Aa | 138.9 ± 0.8 Cb |
Gastric | 75.5 ± 1.7 Ab | 147.8 ± 1.2 Bd | n.d. Aa | n.d. Aa | n.d. Aa | n.d. Aa | 119.2 ± 1.8 Bc | |
Intestinal | 129.4 ± 3.6 Bc | 262.7± 5.7 Cd | n.d. Aa | n.d. Aa | n.d. Aa | n.d. Aa | 76.2 ± 0.5 Ab | |
(all-E)-β-cryptoxanthin | Oral | 201.1 ± 0.3 Bg | 59.4 ± 1.2 Bd | 31.2 ± 0.7 Bb | 38.8 ± 0.9 Cc | 67.5 ± 0.2 Be | 179.0 ± 1.9 Cf | 13.7 ± 0.4 Ba |
Gastric | 181.0 ± 2.0 Ae | 27.7 ± 0.7 Ab | 12.5 ± 0.3 Aa | 23.3 ± 0.1 Ab | 62.5 ± 0.1 Ac | 144.9 ± 2.8 Bd | 6.9 ± 0.2 Aa | |
Intestinal | 232.2 ± 2.6 Cf | 185.1 ± 4.2 Ce | 180.6 ± 1.0 Ce | 30.5 ± 0.8 Bb | 90.9 ± 0.1 Cc | 128.6 ± 0.1 Ad | 15.4 ± 0.2 Ba | |
Total free xanthophylls recovery | ||||||||
Oral phase | 93.5 ± 1.0 Ad | 173.0 ± 3.5 Ce | 47.4 ± 4.1 Ab | 43.2 ± 1.2 Cab | 46.7 ± 0.4 Ab | 83.0 ± 1.1 Bc | 34.3 ± 0.2 Ba | |
Gastric phase | 160.5 ± 8.6 Cd | 47.6 ± 0.2 Aab | 51.9 ± 1.9 Aab | 34.6 ± 0.6 Aa | 92.2 ± 1.1 Ce | 53.5 ± 0.8 Ab | 37.1 ± 0.2 Cab | |
Intestinal phase | 132.8 ± 0.1 Bg | 107.0 ± 1.3 Bf | 83.9 ± 1.7 Be | 37.9 ± 0.1 Bb | 72.1 ± 0.7 Bc | 77.4 ± 1.2 Bd | 27.6 ± 0.4 Aa | |
Xanthophyll esters | ||||||||
(all-E)-lutein-3-O-myristate | Oral | n.d. Aa | 69.2 ± 0.7 Cd | n.d. Aa | n.d. Aa | 3.6 ± 0.1 Ba | n.d. Aa | 75.1 ± 2.4 Cc |
Gastric | n.d. Aa | 32.7 ± 0.6 Bd | n.d. Aa | n.d. Aa | 2.1 ± 0.0 Ab | n.d. Aa | 29.8 ± 0.7 Ac | |
Intestinal | n.d. Aa | 8.2 ± 0.1 Ab | n.d. Aa | n.d. Aa | 41.3 ± 0.3 Cc | n.d. Aa | 40.6 ± 0.9 Bc | |
(9Z)-violaxanthin dimyristate | Oral | 41.1 ± 1.0 Ab | 95.2 ± 0.2 Cf | n.d. Aa | 89.1 ± 0.7 Ce | 55.0 ± 0.2 Ac | 74.4 ± 1.9 Cd | 108.3 ± 2.3 Cg |
Gastric | 91.6 ± 5.2 Bc | 59.9 ± 1.7 Bb | n.d. Aa | 58.2 ± 0.6 Ab | 133.5 ± 0.9 Bd | 51.5 ± 1.4 Ab | 85.6 ± 1.0 Bc | |
Intestinal | 52.7 ± 1.7 Ac | n.d. Aa | n.d. Aa | 69.9 ± 0.3 Be | 58.0 ± 0.8 Ad | 61.5 ± 1.0 Bd | 40.1 ± 1.6 Ab | |
(all-E)-antheraxanthin myristate palmitate | Oral | 103.1 ± 1.9 Bc | 77.2 ± 5.3 Cb | n.d. Aa | 103.9 ± 3.8 Cc | 171.4 ± 7.7 Cd | 219.5 ± 2.2 Ce | 16.6 ± 0.7 Ba |
Gastric | 121.7 ± 0.2 Cb | 52.5 ± 2.1 Bd | n.d. Aa | 77.6 ± 0.5 Be | 120.2 ± 0.3 Bg | 97.5 ± 0.8 Bf | 42.6 ± 1.1 Cc | |
Intestinal | 94.3 ± 0.8 Af | n.d. Aa | n.d. Aa | 40.4 ± 0.2 Ac | 80.1 ± 1.0 Ae | 47.0 ± 0.4 Ad | 11.0 ± 0.0 Ab | |
(all-E)-lutein dimyristate | Oral | 10.0 ± 0.2 Ab | 86.3 ± 1.7 Cf | 0.7 ± 0.0 Aa | 39.7 ± 0.1 Bd | 19.9 ± 0.6 Bc | 72.0 ± 2.6 Ae | 192.8 ± 0.3 Cg |
Gastric | 18.7 ± 1.9 Bb | 51.5 ± 0.3 Bd | 7.4 ± 0.0 Ba | 29.8 ± 0.9 Ac | 12.4 ± 0.7 Aa | 96.8 ± 1.9 Bf | 91.4 ± 0.7 Ae | |
Intestinal | 12.2 ± 0.4 Ab | 25.5 ± 0.2 Ac | 1.4 ± 0.0 Aa | 29.8 ± 0.2 Ad | 63.3 ± 1.0 Ce | 115.5 ± 0.5 Cg | 100.9 ± 0.4 Bf | |
(all-E)-β-cryptoxanthin caprate | Oral | 75.0 ± 2.5 Ac | 59.0± 0.9 Cb | 36.8 ± 0.4 Ba | 110.9 ± 2.2 Cd | 161.0 ± 1.9 Cf | 146.4 ± 3.1 Ce | 31.5 ± 0.2 Ca |
Gastric | 144.9 ± 4.9 Ce | 50.8 ± 1.1 Bb | 37.4 ± 0.2 Ba | 79.1 ± 0.8 Bc | 128.6 ± 2.1 Bd | 119.7 ± 1.9 Bd | 27.9 ± 0.1 Ba | |
Intestinal | 96.2 ± 1.9 Bf | 22.9 ± 0.1 Ab | n.d. Aa | 56.3 ± 0.7 Ad | 63.1 ± 0.9 Ae | 49.1 ± 0.5 Ac | 23.9 ± 0.2 Ab | |
(all-E)-β-cryptoxanthin laurate | Oral | 37.5 ± 0.0 Ab | 65.0 ± 1.3 Cd | 38.4 ± 0.1 Ab | 56.3 ± 0.5 Cc | 112.2 ± 0.3 Cf | 104.3 ± 1.1 Ce | 33.9 ± 0.0 Ca |
Gastric | 96.4 ± 4.5 Cd | 45.9± 1.7 Bb | 37.5 ± 0.0 Ab | 42.0 ± 0.9 Bb | 70.0 ± 0.8 Bc | 77.5 ± 0.5 Bc | 23.7 ± 0.0 Aa | |
Intestinal | 72.0 ± 0.2 Bf | 23.3 ± 0.4 Aa | 76.9 ± 0.4 Bg | 36.9 ± 0.1 Ac | 55.0 ± 0.6 Ae | 40.7 ± 0.4 Ad | 26.0 ± 0.3 Bb | |
(all-E)-β-cryptoxanthin myristate | Oral | 13.5 ± 1.3 Aa | 49.1 ± 0.6 Cc | 28.4 ± 0.4 Ab | 65.1 ± 0.4 Be | 102.2 ± 0.6 Cf | 129.1 ± 1.6 Cg | 54.3 ± 0.1 Cd |
Gastric | 48.0 ± 1.6 Cb | 42.0 ± 0.6 Bb | 84.6 ± 0.5 Cd | 94.3 ± 0.4 Ce | 72.5 ± 3.0 Bc | 68.7 ± 0.5 Bc | 25.3 ± 0.1 Aa | |
Intestinal | 34.9 ± 3.0 Bb | 9.9 ± 0.3 Aa | 62.2 ± 0.3 Bd | 52.2 ± 0.3 Ac | 60.8 ± 0.9 Ad | 35.0 ± 0.4 Ab | 38.6 ± 0.7 Bb | |
(all-E)-β-cryptoxanthin palmitate | Oral | n.d. Aa | 101.7 ± 0.7 Ce | 66.9 ± 0.4 Bb | 94.2 ± 0.0 Cd | 78.2 ± 0.6 Cc | 102.3 ± 0.9 Ce | n.d. Aa |
Gastric | n.d. Aa | 65.5 ± 1.0 Bd | 157.5 ± 1.3 Ce | 21.2± 0.8 Bb | 60.0 ± 0.1 Bc | 61.0 ± 0.2 Bc | n.d. Aa | |
Intestinal | n.d. Aa | 13.0 ± 0.3 Ab | n.d. Aa | 12.1 ± 0.3 Ab | n.d. Aa | 41.7 ± 0.7 Ac | n.d. Aa | |
Total xanthophyll ester recovery | ||||||||
Oral phase | 42.3 ± 0.3 Ac | 73.0 ± 0.1 Cfc | 4.8 ± 0.0 Aa | 38.2 ± 0.1 Bb | 44.0 ± 0.3 Ac | 71.2 ± 0.0 Be | 67.4 ± 0.9 Cd | |
Gastric phase | 78.8 ± 1.5 Ce | 43.2 ± 0.5 Bc | 15.1 ± 0.1 Ca | 27.7 ± 0.1 Ab | 50.2 ± 1.5 Bd | 50.8 ± 0.2 Ad | 41.6 ± 0.1 Bc | |
Intestinal phase | 58.0 ± 0.7 Be | 15.9 ± 0.2 Ab | 6.9 ± 0.0 Ba | 49.7 ± 0.1 Cd | 51.9 ± 1.0 Bd | 69.0 ± 0.5 Be | 37.6 ± 0.5 Ac | |
Hydrocarbon carotenoids | ||||||||
(all-E)-α-carotene | Oral | 218.5 ± 7.2 Be | 43.4 ± 0.5 Ab | 43.7 ± 0.4 Bb | 49.9 ± 0.8 Ab | 86.5 ± 0.1 Cc | 189.7 ± 3.0 Cd | 23.9 ± 0.0 Ca |
Gastric | 245.7± 11.0 Bd | 85.2 ± 0.9 Bc | 22.5 ± 0.7 Aa | 83.6 ± 0.2 Cc | 63.8 ± 0.4 Bb | 94.6 ± 1.8 Bc | 21.1 ± 0.6 Ba | |
Intestinal | 69.6 ± 1.1 Ad | 213.2 ± 1.4 Cf | 201.6 ± 1.2 Ce | 65.7± 0.7 Bd | 25.4 ± 0.1 Ab | 56.1 ± 0.7 Ac | 13.2 ± 0.4 Aa | |
(all-E)-β-carotene | Oral | 50.5 ± 0.5 Ac | 57.7± 0.9 Cd | 43.6 ± 0.6 Bb | 74.4 ± 1.0 Ce | 138.4 ± 1.4 Cf | 215.7 ± 3.3 Cg | 29.6 ± 0.6 Ca |
Gastric | 70.0 ± 1.3 Bf | 32.5 ± 0.9 Ac | 26.1 ± 0.1 Ab | 46.4 ± 0.3 Bd | 51.1 ± 0.4 Ae | 109.5 ± 1.0 Bg | 21.1 ± 0.4 Aa | |
Intestinal | 76.9 ± 0.7 Cg | 37.2 ± 0.3 Bb | 56.8 ± 0.9 Cd | 41.7 ± 0.2 Ac | 66.9 ± 0.7 Bf | 62.3 ± 1.0 Ae | 25.1 ± 0.5 Ba | |
(13Z)-lycopene isomer 2 | Oral | 36.3 ± 1.4 Aa | 77.2 ± 0.0 Bcd | 33.2 ± 0.0 Aa | 74.0 ± 2.0 Bc | 54.2 ± 0.5 Ab | 106.3 ± 2.3 Ce | 80.9 ± 0.3 Cd |
Gastric | 60.9 ± 0.1 Cb | 76.9 ± 0.8 Be | 65.2 ± 0.2 Cc | 35.5 ± 0.9 Aa | 81.2 ± 1.2 Bf | 87.0 ± 1.0 Bg | 70.3 ± 1.1 Bd | |
Intestinal | 54.4 ± 1.5 Bc | 27.4 ± 0.5 Aa | 41.6 ± 0.7 Bb | 85.8 ± 0.9 Ce | 94.9 ± 0.5 Cf | 70.5 ± 0.6 Ad | 46.0 ± 1.8 Ab | |
(9Z)-lycopene isomer 4 | Oral | 86.9 ± 0.3 Ab | 97.8 ± 0.0 Bc | 177.3 ± 0.0 Bf | 126.6 ± 2.2 Ae | 87.5 ± 0.8 Ab | 63.1 ± 0.4 Aa | 111.6 ± 0.4 Ad |
Gastric | 93.0 ± 8.0 Aa | 161.2 ± 1.9 Cc | 229.2 ± 1.2 Cd | 141.6± 1.2 Bb | 217.4 ± 2.2 Cd | 105.9 ± 1.1 Ba | 157.0 ± 1.6 Bc | |
Intestinal | 89.5 ± 1.3 Ac | 57.5 ± 0.3 Ab | 41.8 ± 0.9 Aa | 171.8 ± 0.6 Cf | 144.0 ± 0.4 Bd | 151.7 ± 0.1 Ce | 236.7 ± 0.1 Cg | |
(all-E)-lycopene | Oral | 1.4 ± 0.0 Aa | 29.3 ± 0.9 Ab | 29.9 ± 0.1 Ab | 41.6 ± 0.8 Cc | 69.3 ± 0.9 Bd | 79.5 ± 0.2 Ce | 97.6 ± 1.4 Cf |
Gastric | 29.4 ± 0.1 Bc | 34.8 ± 0.4 Bd | 36.0 ± 0.0 Bd | 38.7 ± 0.0 Be | 20.1 ± 0.4 Aa | 36.8 ± 0.2 Bde | 26.8 ± 1.0 Ab | |
Intestinal | 31.4 ± 0.8 Bb | 35.0 ± 0.4 Bb | 69.2 ± 1.1 Cc | 30.4 ± 0.0 Ab | 71.1 ± 2.2 Bc | 10.0 ± 0.1 Aa | 70.9 ± 0.2 Bc | |
(Z)-lycopene isomer 6 | Oral | 68.9 ± 0.5 Cc | 176.4 ± 2.5 Cf | 76.2 ± 0.3 Bd | 114.7 ± 3.4 Ce | 42.1 ± 0.3 Ab | n.d. Aa | 36.2 ± 0.3 Bb |
Gastric | 42.7 ± 0.0 Bc | 108.5 ± 0.0 Bf | 123.6 ± 0.5 Cg | 35.8 ± 0.7 Ab | 74.5 ± 0.3 Be | n.d. Aa | 56.4 ± 0.1 Cd | |
Intestinal | 18.8 ± 0.5 Ab | 72.0 ± 0.3 Ae | 49.8 ± 0.2 Ac | 65.4 ± 0.7 Bd | 128.1 ± 1.0 Cf | n.d. Aa | n.d. Aa | |
Total hydrocarbon carotenoid recovery | ||||||||
Oral phase | 34.2 ± 0.2 Aa | 39.6 ± 0.1 Bb | 33.3 ± 0.1 Aa | 46.4 ± 0.7 Cc | 72.5 ± 0.2 Bd | 89.2 ± 0.3 Cf | 83.1 ± 0.7 Ce | |
Gastric phase | 37.3 ± 0.3 Bb | 39.6 ± 0.3 Bc | 43.3 ± 0.0 Bd | 39.4 ± 0.1 Bc | 29.8 ± 0.7 Aa | 43.9 ± 0.2 Bd | 29.0 ± 0.3 Aa | |
Intestinal phase | 37.0 ± 0.0 Bc | 34.3 ± 0.0 Ab | 68.0 ± 0.3 Ce | 36.5 ± 0.0 Ac | 71.5 ± 0.4 Bf | 17.7 ± 0.2 Aa | 60.4 ± 0.2 Bd | |
Total carotenoids | ||||||||
Oral phase | 23.4 ± 0.1 Aa | 49.0 ± 0.1 Cc | 22.4 ± 0.2 Aa | 44.2 ± 0.5 Cb | 64.8 ± 0.0 Bd | 84.6 ± 0.1 Cf | 76.4 ± 0.3 Ce | |
Gastric phase | 51.1 ± 0.9 Ce | 40.5 ± 0.3 Bc | 32.5 ± 0.0 Ba | 36.2 ± 0.0 Ab | 36.5 ± 0.8 Ab | 45.6 ± 0.1 Bd | 32.8 ± 0.1 Aa | |
Intestinal phase | 45.1 ± 0.1 Bd | 32.0 ± 0.1 Aa | 44.6 ± 0.0 Cc | 39.9 ± 0.0 Bb | 66.6 ± 0.0 Cf | 31.8 ± 0.1 Aa | 52.6 ± 0.1 Be |
Compound | Non-Treated | 100 MPa/CUT | 100 MPa/5 min | 350 MPa/CUT | 350 MPa/5 min | 600 MPa/CUT | 600 MPa/5 min |
---|---|---|---|---|---|---|---|
Free xanthophylls | |||||||
(all-E)-violaxanthin | 0.6 ± 0.0 a | 1.5 ± 0.0 b | 1.4 ± 0.0 b | 3.2 ± 0.1 e | 2.6 ± 0.1 d | 2.4 ± 0.1 d | 2.0 ± 0.0 c |
(all-E)-zeaxanthin | 1.5 ± 0.0 a | 2.6 ± 0.1 d | 1.7 ± 0.2 ab | 2.1 ± 0.0 bc | 2.4 ± 0.1 cd | 1.3 ± 0.0 a | 1.7 ± 0.0 ab |
(all-E)-β-cryptoxanthin | 3.4 ± 0.1 f | 1.9 ± 0.1 c | n.d. a | 1.4 ± 0.0 b | 2.7 ± 0.1 e | 2.4 ± 0.0 d | 1.7 ± 0.0 c |
Xanthophyll esters | |||||||
(all-E)-β-cryptoxanthin laurate | 0.3 ± 0.0 d | n.d. a | n.d. a | 0.4 ± 0.0 e | 0.3 ± 0.0 d | 0.2 ± 0.0 c | 0.1 ± 0.0 b |
Hydrocarbon carotenoids | |||||||
(all-E)-α-carotene | n.d. a | n.d. a | n.d. a | 2.9 ± 0.0 b | n.d. a | n.d. a | n.d. a |
(all-E)-β-carotene | 0.6 ± 0.0 c | n.d. a | n.d. a | n.d. a | 0.9 ± 0.0 e | 0.8 ± 0.0 d | 0.2 ± 0.0 b |
(13Z)-lycopene isomer 2 | n.d. a | n.d. a | n.d. a | 0.9 ± 0.0 c | 0.4 ± 0.0 b | n.d. a | n.d. a |
(all-E)-lycopene | 0.3 ± 0.0 a | 0.1 ± 0.0 a | 0.1 ± 0.0 a | 0.5 ± 0.0 b | 0.8 ± 0.1 c | 0.2 ± 0.0 a | 0.1 ± 0.0 a |
(Z)-lycopene isomer 6 | n.d. a | 0.4 ± 0.0 c | 0.3 ± 0.0 b | 1.6 ± 0.0 d | n.d. a | n.d. a | n.d. a |
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Lara-Abia, S.; Welti-Chanes, J.; Cano, M.P. Effect of High Hydrostatic Pressure on the Extractability and Bioaccessibility of Carotenoids and Their Esters from Papaya (Carica papaya L.) and Its Impact on Tissue Microstructure. Foods 2021, 10, 2435. https://doi.org/10.3390/foods10102435
Lara-Abia S, Welti-Chanes J, Cano MP. Effect of High Hydrostatic Pressure on the Extractability and Bioaccessibility of Carotenoids and Their Esters from Papaya (Carica papaya L.) and Its Impact on Tissue Microstructure. Foods. 2021; 10(10):2435. https://doi.org/10.3390/foods10102435
Chicago/Turabian StyleLara-Abia, Sara, Jorge Welti-Chanes, and M. Pilar Cano. 2021. "Effect of High Hydrostatic Pressure on the Extractability and Bioaccessibility of Carotenoids and Their Esters from Papaya (Carica papaya L.) and Its Impact on Tissue Microstructure" Foods 10, no. 10: 2435. https://doi.org/10.3390/foods10102435