Next Article in Journal
Porphyromonas gingivalis, Periodontal and Systemic Implications: A Systematic Review
Next Article in Special Issue
Current Concepts of Laser–Oral Tissue Interaction
Previous Article in Journal
Milestones of Dentistry: Advent of Anesthetics in Oral Surgery
Article

Photoinhibition of Streptococcus mutans Biofilm-Induced Lesions in Human Dentin by Violet-Blue Light

1
Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry, Indianapolis, IN 46202, USA
2
Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, Indianapolis, IN 46202, USA
3
Department of Comprehensive Care, Tufts School of Dental Medicine, Boston, MA 02111, USA
4
Department of Biostatistics, Indiana University, Indianapolis, IN 46202, USA
*
Author to whom correspondence should be addressed.
Dent. J. 2019, 7(4), 113; https://doi.org/10.3390/dj7040113
Received: 20 August 2019 / Revised: 29 September 2019 / Accepted: 28 November 2019 / Published: 11 December 2019
(This article belongs to the Special Issue Lasers in Dentistry: Hard and Soft Tissues)
This in vitro study determined the effectiveness of violet-blue light on Streptococcus mutans (UA159) biofilm induced dentinal lesions. Biofilm was formed on human dentin specimens in a 96-well microtiter plate and incubated for 13 h in the presence of tryptic soy broth (TSB) or TSB supplemented with 1% sucrose (TSBS). Violet-blue light (405 nm) from quantitative light-induced fluorescence (QLFTM) was used to irradiate the biofilm. Supernatant liquid was removed, and the biofilm was irradiated continuously with QLF for 5 min twice daily with an interval of 6 h for 5 d, except with one treatment on the final day. Colony forming units (CFU) of the treated biofilm, changes in fluorescence (∆F; QLF-Digital BiluminatorTM), lesion depth (L), and integrated mineral loss (∆Z; both transverse microradiography) were quantified at the end of the fifth day. Statistical analysis used analysis of variance (ANOVA), testing at a 5% significance level. In the violet-blue light irradiated groups, there was a significant reduction (p < 0.05) of bacterial viability (CFU) of S. mutans with TSB and TSBS. Violet-blue light irradiation resulted in the reduction of ∆F and L of the dentinal surface with TSBS. These results indicate that violet-blue light has the capacity to reduce S. mutans cell numbers. View Full-Text
Keywords: violet-blue light; phototherapy; Streptococcus mutans; dental caries violet-blue light; phototherapy; Streptococcus mutans; dental caries
Show Figures

Figure 1

MDPI and ACS Style

Felix Gomez, G.G.; Lippert, F.; Ando, M.; Zandona, A.F.; Eckert, G.J.; Gregory, R.L. Photoinhibition of Streptococcus mutans Biofilm-Induced Lesions in Human Dentin by Violet-Blue Light. Dent. J. 2019, 7, 113. https://doi.org/10.3390/dj7040113

AMA Style

Felix Gomez GG, Lippert F, Ando M, Zandona AF, Eckert GJ, Gregory RL. Photoinhibition of Streptococcus mutans Biofilm-Induced Lesions in Human Dentin by Violet-Blue Light. Dentistry Journal. 2019; 7(4):113. https://doi.org/10.3390/dj7040113

Chicago/Turabian Style

Felix Gomez, Grace G., Frank Lippert, Masatoshi Ando, Andrea F. Zandona, George J. Eckert, and Richard L. Gregory 2019. "Photoinhibition of Streptococcus mutans Biofilm-Induced Lesions in Human Dentin by Violet-Blue Light" Dentistry Journal 7, no. 4: 113. https://doi.org/10.3390/dj7040113

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop