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Article

Platelet Adhesion on Commercially Pure Titanium Plates in Vitro II. Immunofluorescence Visualization of PDGF-B, TGFβ1, and PPARγ Released from Activated Adherent Platelets

1
Tokyo Plastic Dental Society, Kita-ku, Tokyo 114-0002, Japan
2
Private Practice, Takatsu-ku, Kawasaki 213-0033, Japan
3
Division of Anatomy and Cell Biology of the Hard Tissue, Institute of Medicine and Dentistry, Niigata University, 2-5274 Gakkocho-dori, Niigata 951-8514, Japan
4
Department of Oral and Maxillofacial Surgery, Matsumoto Dental University, 1780 Hirooka-gohara, Shiojiri 339-0781, Japan
5
Division of Periodontology, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
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Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata 951-8520, Japan
7
Division of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata University, Niigata 951-8514, Japan
*
Author to whom correspondence should be addressed.
Dent. J. 2019, 7(4), 109; https://doi.org/10.3390/dj7040109
Received: 22 August 2019 / Revised: 14 November 2019 / Accepted: 14 November 2019 / Published: 19 November 2019
Recent progress in the industrial development of dental implants has improved their surface bio-affinity, while clinical implantologists attempt to improve it through coating with various compounds, including platelet-rich plasma (PRP) in clinical settings. However, it is poorly understood how PRP acts on titanium surfaces. To validate this surface modification method and demonstrate how platelet-derived soluble biomolecules released from the activated adherent platelets act on plain, commercially pure-titanium (cp-Ti) plates, we evaluated the distribution of biomolecules by immunofluorescence. PPARγ, PDGF-B, and TGFβ1 were similarly released at immunofluorescence levels from activated adherent platelets, retained in the surrounding extra-platelet spaces for a while, and did not immediately diffuse away to distant spaces. Exogenously added CaCl2 augmented release and retention of those biomolecules along with activation and aggregation. Taken together with our previous data regarding platelet adhesion, these findings suggest that especially when treated with CaCl2, platelets immediately adhere on cp-Ti plates to release their stored biomolecules in the absence of plasma proteins and that these biomolecules do not diffuse away, but stay longer in extra-platelet spaces around the platelets by newly formed, immature fibrin fiber fragments. Consequently, these retained biomolecules are anticipated to cooperatively stabilize implants by stimulating alveolar bone regeneration and integration. View Full-Text
Keywords: platelet-rich plasma; titanium; adhesion; platelet-derived growth factors; transforming growth factor β; peroxisome proliferator-activated receptor γ platelet-rich plasma; titanium; adhesion; platelet-derived growth factors; transforming growth factor β; peroxisome proliferator-activated receptor γ
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MDPI and ACS Style

Tsujino, T.; Takahashi, A.; Watanabe, T.; Isobe, K.; Kitamura, Y.; Okuda, K.; Nakata, K.; Kawase, T. Platelet Adhesion on Commercially Pure Titanium Plates in Vitro II. Immunofluorescence Visualization of PDGF-B, TGFβ1, and PPARγ Released from Activated Adherent Platelets. Dent. J. 2019, 7, 109. https://doi.org/10.3390/dj7040109

AMA Style

Tsujino T, Takahashi A, Watanabe T, Isobe K, Kitamura Y, Okuda K, Nakata K, Kawase T. Platelet Adhesion on Commercially Pure Titanium Plates in Vitro II. Immunofluorescence Visualization of PDGF-B, TGFβ1, and PPARγ Released from Activated Adherent Platelets. Dentistry Journal. 2019; 7(4):109. https://doi.org/10.3390/dj7040109

Chicago/Turabian Style

Tsujino, Tetsuhiro, Akira Takahashi, Taisuke Watanabe, Kazushige Isobe, Yutaka Kitamura, Kazuhiro Okuda, Koh Nakata, and Tomoyuki Kawase. 2019. "Platelet Adhesion on Commercially Pure Titanium Plates in Vitro II. Immunofluorescence Visualization of PDGF-B, TGFβ1, and PPARγ Released from Activated Adherent Platelets" Dentistry Journal 7, no. 4: 109. https://doi.org/10.3390/dj7040109

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