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Dent. J. 2018, 6(4), 48; https://doi.org/10.3390/dj6040048

The Effect of Commercially Available Endodontic Cements and Biomaterials on Osteogenic Differentiation of Dental Pulp Pluripotent-Like Stem Cells

1
UIC Regenerative Medicine Research Institute, Universitat Internacional de Catalunya, St Josep Trueta s/n, Sant Cugat del Vallès, 08195 Barcelona, Spain
2
Oral and Maxillofacial Surgery Department, Fundació Hospital de Nens de Barcelona, 08017 Barcelona, Spain
3
Department of Medical Laboratory Sciences (MLS), Faculty of Allied Health Sciences, Health Sciences Center, Kuwait University, 90805 Sulaibikhat, Kuwait
4
Functional Genomics Unit, Research Division, Dasman Diabetes Institute, 15462 Dasman, Kuwait
*
Authors to whom correspondence should be addressed.
Received: 27 May 2018 / Revised: 3 August 2018 / Accepted: 21 August 2018 / Published: 22 September 2018
(This article belongs to the Section Oral Hygiene, Periodontology and Peri-implant Diseases)
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Abstract

The aim of this study is to compare the osteogenic differentiation capacity of the dental pulp pluripotent-like stem cells (DPPSCs) using conditional media pretreated with ProRoot-MTA, Biodentine (BD) or the newly manufactured pure Portland cement Med-PZ (MZ). DPPSCs, isolated from human third molars, are the most relevant cell model to draw conclusions about the role of biomaterials on dental tissue regeneration. Cytotoxicity, alkaline phosphatase (ALP) activity, and calcium deposition analysis were evaluated at different differentiation time points. Gene expression of key osteogenic markers (RUNX2, Collagen I and Osteocalcin) was determined by qRT-PCR analysis. The osteogenic capacity of cells cultured in conditioned media prepared from MZ or MTA cements was comparable. BD conditioned media supported cell proliferation but failed to induce osteogenesis. Relative to controls and other cements, high osteogenic gene expression was observed in cultures pre-treated with the novel endodontic cement MZ. In conclusion, the in vitro behavior of a MZ- endodontic cement was evaluated, showing similar enhanced cell proliferation compared to other commercially available cements but with an enhanced osteogenic capacity with prospective potential as a novel cement for endodontic treatments. View Full-Text
Keywords: dental pulp pluripotent-like stem cell; dental pulp; pluripotency; Biodentine; MTA; Med-PZ; osteogenic differentiation; biomaterials; osteogenesis dental pulp pluripotent-like stem cell; dental pulp; pluripotency; Biodentine; MTA; Med-PZ; osteogenic differentiation; biomaterials; osteogenesis
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Maher, A.; Núñez-Toldrà, R.; Carrio, N.; Ferres-Padro, E.; Ali, H.; Montori, S.; Al Madhoun, A. The Effect of Commercially Available Endodontic Cements and Biomaterials on Osteogenic Differentiation of Dental Pulp Pluripotent-Like Stem Cells. Dent. J. 2018, 6, 48.

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