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Volume 14
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Article

Experimental Comparative Analysis of the Effectiveness and Cleaning Performance of Conventional and Eco-Friendly Disinfectants Available in Romania

by
Szidonia Krisztina Veress
1,2,
László-István Bába
3,*,
Attila Bitai
4,
Bálint Botond Bögözi
2,
Bernadette Kerekes-Máthé
5,
Dániel Tamás Száva
2 and
Melinda Székely
5
1
Doctoral School of Medicine and Pharmacy, Institution Organizing University Doctoral Studies-IOSUD, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania
2
Department of Oral and Maxilo-Facial Surgery, Faculty of Dentistry, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Str., 540142 Targu Mures, Romania
3
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Str., 540142 Targu Mures, Romania
4
Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Str., 540142 Targu Mures, Romania
5
Department of Teeth and Dental Arches Morphology, Faculty of Dentistry, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 38 Gheorghe Marinescu Str., 540142 Targu Mures, Romania
*
Author to whom correspondence should be addressed.
Dent. J. 2026, 14(3), 159; https://doi.org/10.3390/dj14030159
Submission received: 16 January 2026 / Revised: 20 February 2026 / Accepted: 28 February 2026 / Published: 11 March 2026
(This article belongs to the Topic Advances in Dental Materials)
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Abstract

Background/Objectives: To make a dental office more environmentally conscious, it is essential to use eco-friendly disinfectants without compromising patient safety. Our aim was to examine and compare the effectiveness of the commonly used conventional disinfectants in Romania with the effectiveness of available eco-friendly disinfectants. Methods: Two traditional (Gigasept, Zeta 1 Ultra), and two eco-friendly (IDactiv, Sekusept Aktiv) disinfectants and an eco-friendly (gigazyme) cleaning agent were compared. For a thorough evaluation, minimal inhibitory and bactericidal concentration (MIC/MBC) tests were conducted, along with a Bradford assay to measure the concentration of residual proteins on instruments contaminated by controlled contamination and dental office use after disinfection. Results: During the MIC test, with the exception of Gigazyme®, which is an enzymatic cleaner, all of the tested disinfectants were effective in the case of the bacterial reference strains used at the concentration prescribed by the manufacturers. After disinfection, the amount of protein remaining on the dental instruments did not exceed the maximum concentration according to the ISO 15883-5:2005 protocol, and the protein removal efficiency was between 96.04% and 99.03% for all disinfectants. In the MIC/MBC test, the peracetic acid-based Sekusept Aktiv was the most effective, while Gigazyme® and BossKlein IDactiv® showed the highest protein removal efficiency. Conclusions: The tested eco-friendly disinfectants meet the necessary performance criteria and thus offer a sustainable alternative to traditional disinfectants.
Keywords: disinfection; sustainable development; dental disinfectants; dental instruments; surgical instruments disinfection; sustainable development; dental disinfectants; dental instruments; surgical instruments
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MDPI and ACS Style

Veress, S.K.; Bába, L.-I.; Bitai, A.; Bögözi, B.B.; Kerekes-Máthé, B.; Száva, D.T.; Székely, M. Experimental Comparative Analysis of the Effectiveness and Cleaning Performance of Conventional and Eco-Friendly Disinfectants Available in Romania. Dent. J. 2026, 14, 159. https://doi.org/10.3390/dj14030159

AMA Style

Veress SK, Bába L-I, Bitai A, Bögözi BB, Kerekes-Máthé B, Száva DT, Székely M. Experimental Comparative Analysis of the Effectiveness and Cleaning Performance of Conventional and Eco-Friendly Disinfectants Available in Romania. Dentistry Journal. 2026; 14(3):159. https://doi.org/10.3390/dj14030159

Chicago/Turabian Style

Veress, Szidonia Krisztina, László-István Bába, Attila Bitai, Bálint Botond Bögözi, Bernadette Kerekes-Máthé, Dániel Tamás Száva, and Melinda Székely. 2026. "Experimental Comparative Analysis of the Effectiveness and Cleaning Performance of Conventional and Eco-Friendly Disinfectants Available in Romania" Dentistry Journal 14, no. 3: 159. https://doi.org/10.3390/dj14030159

APA Style

Veress, S. K., Bába, L.-I., Bitai, A., Bögözi, B. B., Kerekes-Máthé, B., Száva, D. T., & Székely, M. (2026). Experimental Comparative Analysis of the Effectiveness and Cleaning Performance of Conventional and Eco-Friendly Disinfectants Available in Romania. Dentistry Journal, 14(3), 159. https://doi.org/10.3390/dj14030159

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