Design and Performance of a Multi-Point Scan Confocal Microendoscope
AbstractConfocal fluorescence microendoscopy provides high-resolution cellular-level imaging via a minimally invasive procedure, but requires fast scanning to achieve real-time imaging in vivo. Ideal confocal imaging performance is obtained with a point scanning system, but the scan rates required for in vivo biomedical imaging can be difficult to achieve. By scanning a line of illumination in one direction in conjunction with a stationary confocal slit aperture, very high image acquisition speeds can be achieved, but at the cost of a reduction in image quality. Here, the design, implementation, and experimental verification of a custom multi-point aperture modification to a line-scanning multi-spectral confocal microendoscope is presented. This new design improves the axial resolution of a line-scan system while maintaining high imaging rates. In addition, compared to the line-scanning configuration, previously reported simulations predicted that the multi-point aperture geometry greatly reduces the effects of tissue scatter on image quality. Experimental results confirming this prediction are presented. View Full-Text
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Risi, M.D.; Makhlouf, H.; Rouse, A.R.; Tanbakuchi, A.A.; Gmitro, A.F. Design and Performance of a Multi-Point Scan Confocal Microendoscope. Photonics 2014, 1, 421-431.
Risi MD, Makhlouf H, Rouse AR, Tanbakuchi AA, Gmitro AF. Design and Performance of a Multi-Point Scan Confocal Microendoscope. Photonics. 2014; 1(4):421-431.Chicago/Turabian Style
Risi, Matthew D.; Makhlouf, Houssine; Rouse, Andrew R.; Tanbakuchi, Anthony A.; Gmitro, Arthur F. 2014. "Design and Performance of a Multi-Point Scan Confocal Microendoscope." Photonics 1, no. 4: 421-431.