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Article

Phytochemical Compound Profile and the Estimation of the Ferruginol Compound in Different Parts (Roots, Leaves, and Seeds) of Juniperus procera

by
Abdalrhaman M. Salih
*,†,
Fahad Al-Qurainy
,
Mohamed Tarroum
,
Salim Khan
,
Mohammad Nadeem
,
Hassan O. Shaikhaldein
and
Saleh Alansi
Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
*
Author to whom correspondence should be addressed.
†
Abdalrhaman M. Salih has contributed significantly to this work.
Separations 2022, 9(11), 352; https://doi.org/10.3390/separations9110352
Submission received: 25 September 2022 / Revised: 19 October 2022 / Accepted: 1 November 2022 / Published: 7 November 2022
(This article belongs to the Special Issue Application of Chromatography in Analytical Chemistry)
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Abstract

:
Secondary plant metabolites and their derivatives play a significant role in human health. Ferruginol is a diterpene phenol that has recently received attention for its pharmacological properties, including antibacterial, antitumor, antimalarial, and cardioprotective effects. Recently, we detected the ferruginol compound in the leaf and seed extracts of Juniperus procera using different analytical approaches. The present work aims at detecting phytochemical compounds in a root extract of J. procera and estimating the amount of ferruginol compound in different parts of Juniperus procera. To screen the phytochemical compounds present in the root extract of J. procera, Gas chromatography/mass spectrometry (GC/MS) was performed. For ferruginol identification and estimation, high-performance liquid chromatography (HPLC) with the ferruginol reference standard and high-resolution direct analysis in real-time (DART) time-of-flight mass spectrometry (TOFMS) (DART-TOF-MS) analysis were used. GC/MS analysis revealed more than 20 bioactive compounds related to secondary plant metabolites in the root extract of J. procera with biological activity. The DART-TOF-MS result showed the typical positive ion spectra of ferruginol, and the HPLC result confirmed that the root extract of J. procera contains the ferruginol compound. In contrast, the root extract of J. procera contained a significant amount of ferruginol compared to that in the leaf and seed extracts. All parts of the J. procera contained the ferruginol compound and proved that ferruginol might be accumulated in the roots, leaves, and seeds of J. procera.

1. Introduction

Juniperus procera Hoechst. ex Endl. is indigenous to the mountains of East Africa from East Sudan to Zimbabwe, and the southern region of the Arab Peninsula across the Red Sea; it is widely spread out throughout the southern part of Saudi Arabia [1,2]. In Saudi Arabia, J. procera is traditionally used for treating jaundice, gastrointestinal disturbances, hepatic diseases, and pharyngitis as an antirheumatic for gout and several inflammatory conditions [3]. Additionally, J. procera is a natural source of photochemical components that may have antioxidant, insecticidal, antibacterial, and anticancer properties [4,5,6,7].
Bioactive components such as phenolics, flavonoids, terpenoids, alkaloids, and their derivatives are very important compounds due to their applications in different fields such as the medical, cosmetic, pharmacological, agricultural, and food industries [8,9,10,11]. Abietane diterpenoids are a type of these bioactive compounds [12,13] that exhibit several interesting biological activities, such as antitumor, antioxidant, antimicrobial, antifungal, antiviral, cytotoxic, and anti-inflammatory activities [14,15]. In this regard, abietane-type diterpenoids are significant phytochemical compounds exhibiting a wide range of pharmacological properties [16]. The pharmacological properties of ferruginol, which include antibacterial, anticancer, antimalarial, and cardioprotective effects, have recently received attention [17,18]. Cancer cell growth is inhibited by the ferruginol compound [18]. Moreover, ferruginol showed a strong protective impact in animal gastric ulcer models [19]. In the literature, the ferruginol compound was widely investigated and showed many important biological activities [16,20,21,22]. A previous study detected ferruginol in the berries of J. procera [23], while we recently detected ferruginol in the leaves and seeds of J. procera through different analytical approaches [24,25], and it is the dominant compound there. In this context, ferruginol is widely distributed in the Juniperus genus [26,27]. Phytochemical components are ubiquitous secondary metabolites in the plant kingdom, and are not nutritional, but vital ingredients for human health maintenance [28]. For better understanding the biological activities that plant species exert, it is important to explore and to evaluate their phytochemical constituents [29]. Different plant parts dominate a pool of bioactive compounds containing potential chemical groups and may contain different amounts of these compounds [30]. New sources of bioactive compounds such as ferruginol are really needed due to their pharmacological properties. Therefore, besides the identification of phytochemical compounds in root extracts of J. procera, this research evaluates the ferruginol compound in different parts (roots, leaves, and seeds) of J. procera. Hence, GC/MS was used for bioactive compound identification from the root extracts, while DART-ToF-MS and HPLC with a ferruginol reference standard were used for the quantification of the ferruginol compound in the different parts (seeds, leaves, and roots) of J. procera.

2. Materials and Methods

2.1. Chemical Reagents

Solvents such as HPLC-grade methanol, acetonitrile, and water were purchased from Sigma-Aldrich, St. Louis, MO, USA), while the ferruginol standard (95%) was purchased from WuXi App Tec- LabNetwork (Shanghai, China).

2.2. Plant Materials and Extraction

Different parts (roots, leaves, and seeds) of J. procera were collected from the alpha region, Saudi Arabia accepting the terms and conditions of national and international standards. A voucher specimen (13,497) was deposited in the herbarium of the center. The collected plant materials were washed carefully using distilled water to remove unwanted particles and dust. Then, plant materials were dried at room temperature. One gram from each part of plant was ground using an electronic blender and placed into 20 mL of methanol. Next, the extraction was carried out using an Innova 44 Inc incubator at 120 rpm, and the temperature was maintained at 28 °C for 5 days. The organic and aqueous phases were separated via centrifugation at 5000 rpm for 15 min. The collected supernatant was filtered through a 0.45 mm nylon syringe and stored at 4 °C for further use.

2.3. GC/MS Analysis of Root Extract

The root extract of J. procera was subjected to GC/MS analysis as follows. Gas chromatography/mass spectrometry (GC/MS) was performed with an Agilent 6890 gas chromatograph (Agilent Inc., Palo Alto, CA, USA) coupled to a 5973MSD operated in electron impact mode at 70 eV ion source energy. The gas chromatograph (GC) was fitted with a 30 m × 0.25 mm fused capillary column coated with 0.25 mm film thick DB-5MS (Agilent), and helium was used as the carrier gas with flow rate 1 mL/min and a split flow of 25 mL/min. The oven temperature was adjusted from 60 °C (1 min initial hold) to 280 °C (isothermal for 10 min final time), with injector temperature of 250 °C and total run time of 50 min. The transfer line temperature was set at 250 °C. The MS was operated in electron impact mode at 70 eV ion source energy. Mass spectrometric data were acquired and processed using the GC-MS ChemStation data system (WILEY 9th edition, NIST-08 MS library, Gaithersburg, MD, USA).

2.4. High-Resolution DART-ToF-MS Analysis

Direct real-time time-of-flight mass spectrometry (DART-ToF-MS) analysis (conditions: ionization ES+ − He; temperature, 250 °C; peak voltage, 500V). AccuTOF LC-plus from JEOL (Tokyo, Japan) was used for extracted sample characterization. Volatile plant extract compounds were evaporated in a stream of helium that had been heated to 250 °C, and ionized with the excited metastable helium atoms before entering the ion source of the time-of-flight mass spectrometer. In positive ionization mode, the molecules were mainly protonated without any fragmentation.

2.5. Quantification of Ferruginol with HPLC

The ferruginol compound was identified and quantified using acetonitrile and mobile-phase methanol (40:60) (v/v). Ferruginol was quantified through HPLC analysis with an Agilent Technologies 1290 Infinity system (Agilent Inc., Palo Alto, CA, USA). The separations were conducted using ZOBRAX RX-C18 column (4.6 × 150 mm) in which the mobile phases were pumped at a flow rate of 1.000 mL/min with a 5 min run time and injection volume of 1 μL. The column temperature was maintained at 27 °C. The chromatogram was recorded at 220 nm. Ferruginol in the sample was identified through its retention time spiked with the ferruginol standard under similar conditions. Ferruginol was estimated by using linear equation y = 967.6x − 44.425 with R2 = 0.9997 on the basis of the reference standard curve prepared with different concentrations of ferruginol (0.2, 0.4, 0.6, and 0.8 µg/mL).

2.6. Statistical Analysis

SPSS one-way analysis of variance (ANOVA)–Duncan’s test was performed for evaluating statistical significance at p < 0.05. The described results represent the mean of three replicates ± standard deviation (SD).

3. Results and Discussion

3.1. GC/MS Analysis of Different Parts of Juniperus procera

J. procera is a natural source of photochemical components that may have antioxidant, insecticidal, antibacterial, and anticancer properties. The methanolic extracts of the leaves and seeds of J. procera were subjected to GC/MS analysis, and the obtained results were published recently [24,31]. In this study, the root extract of J. procera was examined with GC-MS analysis for phytochemical component detection. The bioactive compounds in the root extract were identified using commercial libraries and a comparison of the mass spectra, match percentages, and retention times of the reference compounds. The recorded results of GC analysis reveal that the root extracts of J. procera are rich in phytochemical compounds. In this regard, J. procera is a natural source of phytochemical constituents with potential antimicrobial, insecticidal, anticancer, and antioxidant activities [4,5,6,32]. For accurate results, the methanol that was used as a solvent in the extraction process and the methanolic extract of the root of J. procera were examined with GC/MS analysis (Figure S2 and Figure 1). Then, the detected compounds in the plant sample were compared with the bank (methanol solvent) for bioactive compound identification. GC/MS analysis of the root extract showed about 20 bioactive components related to secondary plant metabolites with biological activity (Figure 1 and Table 1). These detected phytochemical compounds in the root extract of J. procera have antimicrobial, antioxidant, anticancer, and anti-inflammatory biological activities. For example, octanoic acid shows antimicrobial activity [33], while longifolene and undecanoic acid act as antifungal agents [34,35]. The extracted bioactive compounds of J. procera were effective and potent against SCC-9 cancer cells [36]. The phytochemical compounds highlighted above, other phytochemical constituents detected in the root extract of J. procera, and their biological activity are presented in Table 1. These results may support and justify the use of J. procera in traditional medicine.

3.2. Ferruginol Identification and Estimation

Ferruginol is a diterpene phenol that has recently received attention for its pharmacological properties, including antibacterial, antitumor, antimalarial, and cardioprotective effects [17]. In addition, ferruginol inhibits the growth rate of cancer cells [50]. We recently detected the ferruginol compound in leaf and seed extracts of African pencil cedar (J. Procera) through different analytical approaches [24,25,31]. We found that ferruginol is dominant compound in the leaves and seeds of J. procera. The DART-ToF-MS analysis of the extract showed a spectral range from 285.2 to 278.2 (Figure 2 and Table 2), indicating the presence of ferruginol in the extracts of J. procera. This result closely matched the ferruginol compound (C20H30O, mol. wt 286.5) according to the National Institute of Standards and Technology (NIST) Standard Reference Database [51]. Additionally, Kuroda K [52] reported that ferruginol normally generated peaks between 285 and 301. Thus, all these peaks (285.2, 286.2, 287.2) with chemical formulas C20H29O, C20H30O, and C20H31O, and 301. 2 are generated from J. procera tissue using DART-MS might originate from the ferruginol compound (C20H30O, mol. wt. 286.5) (Figure 2 and Table 2), which agreed with previous reports regarding the ferruginol compound [52,53,54]. DART-MS, with the characteristic of the rapid identification of bioactive compounds, is a new research tool for herbal medicine to very simply complete an experimental process [52,55,56]. As the presence of the ferruginol compound was confirmed in the different parts (roots, leaves, and seeds) of J. procera, we separated and evaluated the amount of ferruginol compound in the root, leaf and seed extracts of J. Procera. However, different factors, such as sample preparation, mobile phase, column types, and detectors should be considered for the chromatographic analysis of phenolic compounds [57]. Hence, for the separation of the ferruginol compound, HPLC with an authentic standard, and different mobile phases and conditions were tested. Among the tested mobile phases, the methanol and acetonitrile (60:40) (v/v) combination was suitable for ferruginol separation considering the conditions detailed in the methodology section (Figure 3a). In this regard, methanol and acetonitrile or their aqueous forms are the main mobile phases utilized in the HPLC quantification of phenolic compounds [58]. Ferruginol in the plant materials was identified via its retention time and by spiking with a reference standard under similar conditions, whereas ferruginol was quantified using a linear equation prepared with different concentrations of the standard (Figure S1).
The obtained results confirm that all parts (roots, leaves, and seeds) of Juniperus procera contain the ferruginol compound. These results also demonstrate that, among the different parts (leaves, seeds, and roots) of J. procera, the root extract contained a higher amount of the ferruginol compound (4.4 µg), followed by the leaf (0.43 µg) and seed (0.42 µg) extracts (Figure 3b). In the literature, the ferruginol compound was detected in the berries of J. procera [23], but no previous report examined the quantification of the ferruginol compound in the different parts (leaves, seeds, and roots) of J. procera or any related plant as a comparative study. However, the authors in [53] stated that ferruginol was distributed in heartwood tissue or accumulated in intermediate wood and heartwood [52]. This study proves that the ferruginol compound might be accumulated in different plant parts. Moreover, this research reveals that the roots of J. procera are a new source of ferruginol.

4. Conclusions

In conclusion, this study confirmed that J. procera is rich in bioactive compounds, and that all parts (roots, leaves, and seeds) of J. procera contain the ferruginol compound. This research also revealed that the root extracts contained a significantly greater amount of ferruginol compared to that in other parts of the plant. Moreover, this research demonstrated that the mobile phase consisting of methanol and acetonitrile is suitable for the chromatographic separation of the ferruginol compound. Thus, these results support and justify the utilization of J. procera in traditional medicine. Further, research should be conducted to investigate the medicinal value of the root extract of J. procera.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/separations9110352/s1. Figure S1: standard ferruginol curve; Figure S2: GC-MS chromatogram of blank (methanol).

Author Contributions

A.M.S. proposed the work, and planned and performed the experiments; A.M.S., M.T., S.K., M.N., H.O.S. and S.A., methodology; F.A.-Q., lab supervisor; A.M.S. wrote the full manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Data are available from the correspondence.

Acknowledgments

The authors extend their appreciation to researchers supporting project number RSP-2021/73 at King Saud University, Riyadh, Saudi Arabia.

Conflicts of Interest

The authors have declared no conflict of interest.

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Separations 09 00352 g001 550
Figure 1. GC-MS chromatogram of the root extract of J. procera.
Figure 1. GC-MS chromatogram of the root extract of J. procera.
Separations 09 00352 g001
Separations 09 00352 g002 550
Figure 2. DART TOF-MS chromatogram of J. procera showing the ferruginol spectrum.
Figure 2. DART TOF-MS chromatogram of J. procera showing the ferruginol spectrum.
Separations 09 00352 g002
Separations 09 00352 g003 550
Figure 3. (a) Ferruginol standard (RT = 2.498); (b) concentration of ferruginol in different part of Juniperus procera. a,b different superscripts within the same column differ significantly (p < 0.05). Reported results represent the mean of three replicates ± standard deviation (SD).
Figure 3. (a) Ferruginol standard (RT = 2.498); (b) concentration of ferruginol in different part of Juniperus procera. a,b different superscripts within the same column differ significantly (p < 0.05). Reported results represent the mean of three replicates ± standard deviation (SD).
Separations 09 00352 g003
Table
Table 1. Secondary gas-chromatography/mass-spectrometry metabolites profile and biological activity of the root extract of J. procera.
Table 1. Secondary gas-chromatography/mass-spectrometry metabolites profile and biological activity of the root extract of J. procera.
Compound NameRTAreaMol. Wt.Biological Activity
Octanoic acid12.042283.354158.131Antimicrobial [33].
Dodecane13.947188.425170.203-
1,2,4-Metheno-1H-indene 17.981149.130204.188-
1H-Cycloprop[e]azulene 18.485233.181204.188Analgesic and anti-inflammatory activities [37].
Longifolene18.8972.433204.188Antifungal [34].
Undecanoic acid19.028164.046200.178Antifungal [35].
Thunbergol19.429104.044290.261Antimicrobial activity [38].
Humulene19.85284.054204.188Anti-inflammatory [39].
(E,Z)-.alpha.-Farnesene20.173648.868204.188Antibacterial activity [40].
(1R,4R,5S)-1,8-Dimethyl-4-(prop-1-en-2-yl)spiro20.247242.138204.188-
beta-Humulene20.55214.483204.188Antibacterial activity [41].
2,4-Di-tert-butylphenol20.762160.545206.167Antifungal [42].
Cycloisolongifolene, 8,9-dehydro-21.42125.370202.172Antibacterial and antifungal activities [43].
2,5-di-tert-Butyl-1,4-benzoquinone21.8614.120220.146-
Tridecanoic acid23.10222.647228.209Anthelminthic,
anti-inflammatory, antimicrobial, and anticancerous [44].
2-Tridecanone28.092201.866198.198Insecticidal activity [45].
cis-10-Heptadecenoic acid28.286147.813282.256Antioxidant [46].
Hexadecanoic acid29.654167.380284.272Anti-inflammatory [47].
9-Octadecenoic acid 31.365980.013296.272Anti-inflammatory [48].
Nonadecanoic acid33.173178.676312.303-
2-Phenanthrenol 34.398840.324286.23Antimicrobial [48].
Docosanoic acid 37.316196.258354.35Antioxidant [49].
Table
Table 2. Main phytochemical compound of the extracts of J. procera detected with DART-ToF-MS, indicating the presence of ferruginol in the extracts.
Table 2. Main phytochemical compound of the extracts of J. procera detected with DART-ToF-MS, indicating the presence of ferruginol in the extracts.
No.Experimental MassCalculated MassMass Difference
(mmu)		FormulaUnsaturation DegreePossible Compound
1285.22493285.221843.09C20H29O6.5Ferruginol
2286.22961286.22968–0.05C20H30O6.0
3287.23777287.237490.28C20H31O5.5
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Salih, A.M.; Al-Qurainy, F.; Tarroum, M.; Khan, S.; Nadeem, M.; Shaikhaldein, H.O.; Alansi, S. Phytochemical Compound Profile and the Estimation of the Ferruginol Compound in Different Parts (Roots, Leaves, and Seeds) of Juniperus procera. Separations 2022, 9, 352. https://doi.org/10.3390/separations9110352

AMA Style

Salih AM, Al-Qurainy F, Tarroum M, Khan S, Nadeem M, Shaikhaldein HO, Alansi S. Phytochemical Compound Profile and the Estimation of the Ferruginol Compound in Different Parts (Roots, Leaves, and Seeds) of Juniperus procera. Separations. 2022; 9(11):352. https://doi.org/10.3390/separations9110352

Chicago/Turabian Style

Salih, Abdalrhaman M., Fahad Al-Qurainy, Mohamed Tarroum, Salim Khan, Mohammad Nadeem, Hassan O. Shaikhaldein, and Saleh Alansi. 2022. "Phytochemical Compound Profile and the Estimation of the Ferruginol Compound in Different Parts (Roots, Leaves, and Seeds) of Juniperus procera" Separations 9, no. 11: 352. https://doi.org/10.3390/separations9110352

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