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Article

HPLC-DAD Analysis of Hemp Oil Supplements for Determination of Four Cannabinoids: Cannabidiol, Cannabidiolic Acid, Cannabinol and Delta 9-Tetrahydrocannabinol

1
Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 30-387 Krakow, Poland
2
School of Biomedicine, Far Eastern Federal University, 690090 Vladivostok, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Daniele Naviglio
Separations 2021, 8(12), 227; https://doi.org/10.3390/separations8120227
Received: 14 October 2021 / Revised: 8 November 2021 / Accepted: 17 November 2021 / Published: 23 November 2021
(This article belongs to the Special Issue Separation and Quantitative Analysis of Natural Product Extracts)
Growing consumer interest in hemp oilseed supplements requires quality control. Therefore, appropriate, effective and verified analytical methods are needed for the determination of some bioactive cannabinoids in them. The aim of the study is to present an extended (compared to our previous research) validated high performance liquid chromatography with diode array detection (HPLC-DAD) method for the determination of four cannabinoids (cannabidiol, cannabidiolic acid, cannabinol and delta-9-tetrahydrocannabinol) in an oil matrix, which was used to determine these cannabinoids in seven commercial hemp oil supplements. In our method, the isolation of the target compounds was based on liquid extraction with acetonitrile combined with the freezing (at −41 °C) of the oil phase. The results show that in some cases, the determined concentrations of cannabinoids in the tested supplements differ significantly from those declared by the manufacturers. As for the main medicinal cannabinoid (CBD) in hemp oil supplements, in two cases, the measured concentration was significantly lower (1.45 and 1.81%) than the declared (5 and 5%), and in the other supplements, the obtained results confirm the declared amount of CBD within the error range from 3.29 to 9.2%. Therefore, to ensure the safe and beneficial use of these supplements by consumers, it is necessary to monitor their cannabinoid composition. View Full-Text
Keywords: cannabinoids determination; hemp oil supplements; validated HPLC-DAD method cannabinoids determination; hemp oil supplements; validated HPLC-DAD method
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MDPI and ACS Style

Madej, K.; Chmiołek, A.; Szlachta, K.; Piekoszewski, W. HPLC-DAD Analysis of Hemp Oil Supplements for Determination of Four Cannabinoids: Cannabidiol, Cannabidiolic Acid, Cannabinol and Delta 9-Tetrahydrocannabinol. Separations 2021, 8, 227. https://doi.org/10.3390/separations8120227

AMA Style

Madej K, Chmiołek A, Szlachta K, Piekoszewski W. HPLC-DAD Analysis of Hemp Oil Supplements for Determination of Four Cannabinoids: Cannabidiol, Cannabidiolic Acid, Cannabinol and Delta 9-Tetrahydrocannabinol. Separations. 2021; 8(12):227. https://doi.org/10.3390/separations8120227

Chicago/Turabian Style

Madej, Katarzyna, Aleksandra Chmiołek, Kamila Szlachta, and Wojciech Piekoszewski. 2021. "HPLC-DAD Analysis of Hemp Oil Supplements for Determination of Four Cannabinoids: Cannabidiol, Cannabidiolic Acid, Cannabinol and Delta 9-Tetrahydrocannabinol" Separations 8, no. 12: 227. https://doi.org/10.3390/separations8120227

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