Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot
Abstract
:1. Introduction
2. Materials and Methods
2.1. Specimens and Storage
2.2. Reagents
2.3. Sample Preparation for Extraction and Derivatization
2.4. LC-MS/MS Analysis
2.5. Real-Time Stability Monitoring
2.6. Statistical Analysis
3. Results
3.1. Characteristics of Routine Practice
3.2. Method Validation
3.3. Comparison between the Initial Data and Peer Group Data with Derivatized-MS/MS Non-Kit
3.4. Comparison of the Initial Data and Data within the Shelf Life or after the Shelf Life
3.5. Real-Time Stability Analysis
3.6. Correlation Analysis Aetween Aime and Analytes
4. Discussion
5. Conclusions
Supplementary Materials
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Analyte | Level | Initial | Within the Shelf Life | After the Shelf Life | Total | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
D0 to D60 (n = 25) | D0 to D364 (n = 151) | D365 to D788 (n = 179) | D0 to D788 (n = 330) | |||||||||||||
(μmol/L) | Mean | SD | CV | Mean | SD | CV | p | Mean | SD | CV | p | Mean | SD | CV | Range | |
Phe | low | 89.7 | 10.5 | 11.7% | 91.5 | 8.1 | 8.9% | N.S | 95.9 | 7.5 | 7.8% | <0.001 | 93.9 | 8.1 | 8.6% | 76.0 to 116.4 |
medium | 256.9 | 35.3 | 13.7% | 256.7 | 24.4 | 9.5% | N.S | 269.5 | 22.7 | 8.4% | 0.017 | 263.7 | 24.3 | 9.2% | 205.0 to 336.8 | |
high | 459.7 | 50.2 | 10.9% | 471.5 | 39.9 | 8.5% | N.S | 495.1 | 47.5 | 9.6% | <0.001 | 484.3 | 45.8 | 9.4% | 377.0 to 596.1 | |
Leu | low | 153.2 | 15.1 | 9.9% | 161.8 | 13.1 | 8.1% | 0.003 | 169.1 | 13.9 | 8.2% | <0.001 | 165.8 | 14.0 | 8.5% | 135.0 to 212.4 |
medium | 343.6 | 37.1 | 10.8% | 347.8 | 32.5 | 9.3% | N.S | 358.7 | 31.9 | 8.9% | 0.031 | 353.7 | 32.5 | 9.2% | 276.0 to 436.4 | |
high | 560.6 | 48.6 | 8.7% | 584.7 | 51.3 | 8.8% | 0.030 | 599.1 | 57.0 | 9.5% | 0.002 | 592.5 | 54.7 | 9.2% | 472.0 to 727.1 | |
Met | low | 23.6 | 2.4 | 10.3% | 23.1 | 2.3 | 9.9% | N.S | 23.6 | 2.1 | 9.1% | N.S | 23.4 | 2.2 | 9.5% | 19.0 to 27.9 |
medium | 82.0 | 8.9 | 10.9% | 78.8 | 7.2 | 9.2% | 0.049 | 78.4 | 7.8 | 10.0% | 0.035 | 78.6 | 7.5 | 9.6% | 59.0 to 103.2 | |
high | 191.2 | 18.7 | 9.8% | 189.4 | 17.9 | 9.5% | N.S | 184.2 | 17.6 | 9.5% | N.S | 186.6 | 17.9 | 9.6% | 151.0 to 225.1 | |
Tyr | low | 66.6 | 6.7 | 10.0% | 65.7 | 5.9 | 9.0% | N.S | 66.7 | 6.4 | 9.5% | N.S | 66.2 | 6.2 | 9.3% | 51.0 to 84.5 |
medium | 313.2 | 31.4 | 10.0% | 310.0 | 26.9 | 8.7% | N.S | 316.3 | 27.3 | 8.6% | N.S | 313.4 | 27.3 | 8.7% | 253.0 to 395.9 | |
high | 502.5 | 46.4 | 9.2% | 500.2 | 42.3 | 8.5% | N.S | 507.8 | 48.0 | 9.4% | N.S | 504.3 | 45.6 | 9.0% | 391.0 to 624.8 | |
Val | low | 173.3 | 16.8 | 9.7% | 179.0 | 16.4 | 9.2% | N.S | 182.7 | 15.2 | 8.3% | 0.005 | 181.0 | 15.9 | 8.8% | 149.0 to 235.0 |
medium | 384.1 | 37.7 | 9.8% | 385.5 | 36.6 | 9.5% | N.S | 395.7 | 39.6 | 10.0% | N.S | 391.0 | 38.6 | 9.9% | 302.0 to 488.9 | |
high | 644.2 | 56.7 | 8.8% | 663.0 | 57.4 | 8.7% | N.S | 669.7 | 62.7 | 9.4% | N.S | 666.6 | 60.3 | 9.0% | 537.0 to 831.6 | |
Cit | low | 26.9 | 2.2 | 8.0% | 27.5 | 1.9 | 7.0% | N.S | 28.0 | 1.7 | 6.3% | 0.004 | 27.8 | 1.8 | 6.7% | 21.0 to 33.6 |
medium | 78.7 | 6.7 | 8.5% | 78.7 | 5.9 | 7.5% | N.S | 78.7 | 4.9 | 6.2% | N.S | 78.7 | 5.4 | 6.9% | 58.0 to 97.3 | |
high | 169.7 | 12.4 | 7.3% | 174.5 | 12.6 | 7.2% | N.S | 172.9 | 10.5 | 6.1% | N.S | 173.7 | 11.5 | 6.6% | 135.0 to 207.0 | |
Arg | low | 9.0 | 0.9 | 9.7% | 9.9 | 0.9 | 9.4% | <0.001 | 10.6 | 1.0 | 9.1% | <0.001 | 10.3 | 1.0 | 9.8% | 7.3 to 12.5 |
medium | 28.4 | 3.3 | 11.8% | 29.2 | 2.7 | 9.2% | N.S | 27.5 | 2.4 | 8.9% | N.S | 28.3 | 2.7 | 9.5% | 21.0 to 34.4 | |
high | 40.0 | 3.7 | 9.2% | 39.6 | 4.2 | 10.5% | N.S | 30.8 | 2.8 | 9.2% | <0.001 | 34.8 | 5.6 | 16.2% | 23.0 to 49.1 | |
C0 | low | 39.7 | 3.6 | 9.0% | 40.3 | 3.1 | 7.7% | N.S | 41.3 | 2.7 | 6.5% | 0.009 | 40.9 | 2.9 | 7.2% | 34.0 to 48.6 |
medium | 56.8 | 5.0 | 8.8% | 57.5 | 5.0 | 8.7% | N.S | 57.7 | 4.6 | 7.9% | N.S | 57.6 | 4.8 | 8.3% | 45.0 to 72.1 | |
high | 76.5 | 5.4 | 7.0% | 76.8 | 5.7 | 7.4% | N.S | 76.7 | 7.0 | 9.1% | N.S | 76.8 | 6.4 | 8.4% | 60.0 to 97.4 | |
C2 | low | 25.2 | 1.9 | 7.6% | 25.2 | 1.9 | 7.7% | N.S | 25.1 | 1.8 | 7.2% | N.S | 25.1 | 1.9 | 7.4% | 20.0 to 30.0 |
medium | 34.2 | 2.3 | 6.8% | 34.1 | 2.5 | 7.4% | N.S | 33.6 | 2.8 | 8.3% | N.S | 33.8 | 2.7 | 7.9% | 27.0 to 42.3 | |
high | 44.0 | 2.9 | 6.5% | 43.2 | 2.6 | 5.9% | N.S | 41.5 | 3.6 | 8.7% | 0.002 | 42.3 | 3.3 | 7.8% | 34.0 to 51.6 | |
C3 | low | 5.0 | 0.5 | 9.4% | 5.1 | 0.4 | 7.7% | N.S | 5.2 | 0.3 | 6.5% | 0.010 | 5.1 | 0.4 | 7.1% | 4.2 to 6.1 |
medium | 10.3 | 0.8 | 7.5% | 10.4 | 0.8 | 7.8% | N.S | 10.2 | 0.8 | 7.5% | N.S | 10.3 | 0.8 | 7.7% | 8.4 to 13.0 | |
high | 15.5 | 1.0 | 6.7% | 15.6 | 1.0 | 6.7% | N.S | 15.3 | 1.2 | 8.2% | N.S | 15.4 | 1.2 | 7.6% | 13.0 to 18.7 | |
C4 | low | 0.9 | 0.1 | 9.2% | 0.9 | 0.1 | 10.5% | N.S | 0.9 | 0.1 | 11.4% | N.S | 0.9 | 0.1 | 11.0% | 0.7 to 1.1 |
medium | 1.9 | 0.3 | 13.6% | 2.0 | 0.2 | 10.4% | N.S | 1.9 | 0.2 | 10.3% | N.S | 2.0 | 0.2 | 10.4% | 1.5 to 2.5 | |
high | 3.9 | 0.5 | 11.8% | 3.8 | 0.4 | 10.9% | N.S | 3.7 | 0.4 | 10.5% | N.S | 3.7 | 0.4 | 10.8% | 2.8 to 4.6 | |
C5 | low | 0.6 | 0.1 | 15.7% | 0.6 | 0.1 | 11.7% | N.S | 0.6 | 0.1 | 10.7% | N.S | 0.6 | 0.1 | 11.1% | 0.4 to 0.7 |
medium | 1.5 | 0.2 | 14.5% | 1.5 | 0.2 | 10.7% | N.S | 1.5 | 0.2 | 10.4% | N.S | 1.5 | 0.2 | 10.5% | 1.2 to 1.8 | |
high | 2.7 | 0.2 | 9.1% | 2.7 | 0.3 | 9.2% | N.S | 2.8 | 0.3 | 9.7% | N.S | 2.8 | 0.3 | 9.5% | 2.1 to 3.3 | |
C5DC | low | 0.4 | 0.1 | 14.3% | 0.4 | 0.0 | 11.8% | N.S | 0.4 | 0.0 | 11.8% | N.S | 0.4 | 0.0 | 11.9% | 0.3 to 0.5 |
medium | 0.7 | 0.1 | 11.0% | 0.7 | 0.1 | 10.3% | N.S | 0.7 | 0.1 | 12.1% | N.S | 0.7 | 0.1 | 11.3% | 0.5 to 0.9 | |
high | 1.9 | 0.2 | 10.8% | 1.8 | 0.2 | 11.1% | N.S | 1.7 | 0.2 | 11.5% | <0.001 | 1.7 | 0.2 | 11.7% | 1.2 to 2.2 | |
C5OH | low | 0.8 | 0.1 | 11.1% | 0.8 | 0.1 | 9.8% | N.S | 0.8 | 0.1 | 10.1% | N.S | 0.8 | 0.1 | 10.0% | 0.6 to 1.0 |
medium | 1.2 | 0.2 | 14.7% | 1.2 | 0.2 | 12.4% | N.S | 1.2 | 0.1 | 10.1% | N.S | 1.2 | 0.1 | 11.2% | 0.9 to 1.5 | |
high | 1.7 | 0.2 | 12.3% | 1.7 | 0.2 | 10.8% | N.S | 1.7 | 0.2 | 10.3% | N.S | 1.7 | 0.2 | 10.5% | 1.3 to 2.1 | |
C6 | low | 0.5 | 0.1 | 12.7% | 0.5 | 0.1 | 12.1% | N.S | 0.5 | 0.1 | 11.6% | N.S | 0.5 | 0.1 | 11.8% | 0.4 to 0.6 |
medium | 0.8 | 0.1 | 14.1% | 0.9 | 0.1 | 12.0% | N.S | 0.9 | 0.1 | 11.5% | N.S | 0.9 | 0.1 | 11.7% | 0.7 to 1.1 | |
high | 2.0 | 0.2 | 10.2% | 2.1 | 0.2 | 10.5% | N.S | 2.0 | 0.2 | 10.1% | N.S | 2.0 | 0.2 | 10.3% | 1.6 to 2.5 | |
C8 | low | 0.6 | 0.1 | 9.2% | 0.6 | 0.1 | 10.5% | N.S | 0.6 | 0.1 | 10.6% | N.S | 0.6 | 0.1 | 10.5% | 0.5 to 0.9 |
medium | 1.1 | 0.1 | 12.4% | 1.1 | 0.1 | 10.8% | N.S | 1.1 | 0.1 | 10.0% | N.S | 1.1 | 0.1 | 10.4% | 0.9 to 1.4 | |
high | 2.8 | 0.3 | 10.5% | 2.8 | 0.3 | 10.1% | N.S | 2.7 | 0.3 | 11.1% | N.S | 2.8 | 0.3 | 10.7% | 2.1 to 3.5 | |
C10 | low | 0.4 | 0.0 | 13.4% | 0.4 | 0.0 | 11.1% | N.S | 0.4 | 0.0 | 11.5% | N.S | 0.4 | 0.0 | 11.3% | 0.3 to 0.5 |
medium | 1.1 | 0.1 | 13.3% | 1.1 | 0.1 | 11.0% | N.S | 1.1 | 0.1 | 11.2% | N.S | 1.1 | 0.1 | 11.1% | 0.8 to 1.4 | |
high | 2.2 | 0.3 | 13.4% | 2.2 | 0.2 | 11.1% | N.S | 2.2 | 0.2 | 11.1% | N.S | 2.2 | 0.2 | 11.1% | 1.6 to 2.8 | |
C12 | low | 0.4 | 0.1 | 14.7% | 0.4 | 0.0 | 10.7% | N.S | 0.4 | 0.0 | 11.8% | N.S | 0.4 | 0.0 | 11.5% | 0.3 to 0.5 |
medium | 0.7 | 0.1 | 11.8% | 0.7 | 0.1 | 11.1% | N.S | 0.7 | 0.1 | 10.3% | N.S | 0.7 | 0.1 | 10.9% | 0.5 to 0.9 | |
high | 1.5 | 0.1 | 9.1% | 1.5 | 0.2 | 10.1% | N.S | 1.6 | 0.2 | 10.2% | 0.029 | 1.5 | 0.2 | 10.3% | 1.1 to 1.9 | |
C14 | low | 0.6 | 0.1 | 10.7% | 0.6 | 0.1 | 9.4% | N.S | 0.6 | 0.1 | 11.3% | N.S | 0.6 | 0.1 | 10.4% | 0.5 to 0.8 |
medium | 1.6 | 0.3 | 15.8% | 1.7 | 0.2 | 11.4% | N.S | 1.7 | 0.2 | 9.9% | N.S | 1.7 | 0.2 | 10.7% | 1.3 to 2.1 | |
high | 3.3 | 0.4 | 10.5% | 3.3 | 0.3 | 8.9% | N.S | 3.4 | 0.4 | 10.4% | N.S | 3.4 | 0.3 | 9.8% | 2.7 to 4.2 | |
C16 | low | 4.6 | 0.4 | 8.4% | 4.8 | 0.4 | 8.8% | N.S | 4.8 | 0.4 | 9.1% | N.S | 4.8 | 0.4 | 8.9% | 3.9 to 5.9 |
medium | 8.2 | 0.8 | 9.2% | 8.5 | 0.8 | 9.1% | N.S | 8.5 | 0.8 | 9.9% | N.S | 8.5 | 0.8 | 9.5% | 6.6 to 10.5 | |
high | 11.5 | 1.4 | 12.3% | 11.8 | 1.2 | 9.7% | N.S | 12.0 | 1.2 | 10.1% | N.S | 11.9 | 1.2 | 9.9% | 8.4 to 14.9 | |
C18 | low | 1.9 | 0.2 | 11.3% | 1.9 | 0.2 | 9.8% | N.S | 1.9 | 0.2 | 10.0% | N.S | 1.9 | 0.2 | 9.9% | 1.5 to 2.4 |
medium | 3.0 | 0.4 | 12.1% | 3.0 | 0.3 | 10.6% | N.S | 3.0 | 0.3 | 10.6% | N.S | 3.0 | 0.3 | 10.6% | 2.2 to 3.7 | |
high | 6.1 | 0.7 | 11.0% | 6.2 | 0.6 | 9.7% | N.S | 6.3 | 0.6 | 9.4% | N.S | 6.2 | 0.6 | 9.5% | 5.0 to 7.7 |
Analyte (μmol/L) | Level | Slope | Intercept | SE Slope | F | p Value | eD542 (μmol/L) | %eD542 | %D542adj |
---|---|---|---|---|---|---|---|---|---|
Phe | low | 0.012 | 89.4 | 0.002 | 38.6 | <0.001 | 95.6 | 6.6% | 2.0% |
medium | 0.029 | 252.3 | 0.006 | 25.7 | <0.001 | 267.9 | 4.3% | 1.4% | |
high | 0.056 | 462.2 | 0.011 | 27.7 | <0.001 | 492.6 | 7.2% | 1.5% | |
Leu | low | 0.022 | 157.2 | 0.003 | 46.8 | <0.001 | 169.0 | 10.3% | 0.1% |
medium | 0.032 | 341.1 | 0.008 | 17.3 | <0.001 | 358.4 | 4.3% | −0.5% | |
high | 0.048 | 573.4 | 0.013 | 13.9 | <0.001 | 599.7 | 7.0% | 0.3% | |
Met | low | 0.001 | 23.2 | 0.001 | 1.0 | 0.329 | 23.4 | −0.9% | 0.3% |
medium | −0.003 | 79.6 | 0.002 | 2.0 | 0.158 | 78.2 | −4.6% | −1.8% | |
high | −0.010 | 190.3 | 0.004 | 4.9 | 0.027 | 185.1 | −3.2% | 0.5% | |
Tyr | low | 0.004 | 64.8 | 0.001 | 5.8 | 0.016 | 66.7 | 0.2% | −7.2% |
medium | 0.019 | 306.1 | 0.007 | 8.1 | 0.005 | 316.2 | 1.0% | −12.1% | |
high | 0.025 | 494.3 | 0.011 | 5.3 | 0.022 | 508.0 | 1.1% | −5.8% | |
Val | low | 0.014 | 175.4 | 0.004 | 14.3 | 0.000 | 183.1 | 5.6% | 13.4% |
medium | 0.025 | 381.2 | 0.009 | 7.3 | 0.007 | 394.7 | 2.8% | 1.6% | |
high | 0.041 | 650.2 | 0.014 | 8.3 | 0.004 | 672.7 | 4.4% | −4.8% | |
Cit | low | 0.002 | 27.2 | 0.000 | 12.3 | 0.001 | 28.0 | 3.9% | 7.8% |
medium | 0.002 | 78.0 | 0.001 | 1.6 | 0.203 | 78.9 | 0.3% | −1.8% | |
high | 0.001 | 173.1 | 0.003 | 0.3 | 0.616 | 173.9 | 2.4% | −6.9% | |
Arg | low | 0.001 | 9.8 | 0.000 | 23.6 | <0.001 | 10.5 | 15.9% | 30.2% |
medium | −0.004 | 30.0 | 0.001 | 51.5 | <0.001 | 27.7 | −2.7% | 0.6% | |
high | −0.019 | 42.5 | 0.001 | 507.3 | <0.001 | 32.0 | −20.1% | −24.5% | |
C0 | low | 0.003 | 39.7 | 0.002 to 0.004 | 20.5 | <0.001 | 41.3 | 4.1% | 1.6% |
medium | 0.001 | 57.2 | −0.001 to 0.003 | 1.0 | 0.314 | 57.8 | 1.7% | 0.1% | |
high | 0.003 | 75.7 | −0.000 to 0.006 | 3.0 | 0.082 | 77.2 | 0.9% | −2.8% | |
C2 | low | −0.001 | 25.5 | −0.002 to 0.000 | 4.2 | 0.041 | 25.0 | −1.0% | 2.0% |
medium | −0.003 | 34.9 | −0.004 to −0.001 | 17.6 | <0.001 | 33.4 | −2.4% | 3.1% | |
high | −0.004 | 43.9 | −0.006 to −0.003 | 29.6 | <0.001 | 41.7 | −5.2% | −3.4% | |
C3 | low | 0.000 | 5.0 | 0.000 | 7.6 | 0.006 | 5.2 | 3.9% | 5.6% |
medium | 0.000 | 10.4 | −0.001 | 3.8 | 0.051 | 10.2 | −1.0% | 2.0% | |
high | 0.000 | 15.5 | −0.001 | 0.8 | 0.374 | 15.4 | −0.8% | −3.4% | |
C4 | low | 0.000 | 0.9 | 0.000 | 0.7 | 0.397 | 0.9 | 3.0% | 8.9% |
medium | 0.000 | 2.0 | 0.000 | 0.6 | 0.445 | 2.0 | 1.8% | 2.3% | |
high | 0.000 | 3.8 | 0.000 | 7.6 | 0.006 | 3.7 | −5.0% | −10.6% | |
C5 | low | 0.000 | 0.6 | 0.000 | 1.0 | 0.328 | 0.6 | −0.4% | −0.7% |
medium | 0.000 | 1.5 | 0.000 | 0.1 | 0.717 | 1.5 | −1.0% | 3.8% | |
high | 0.000 | 2.7 | 0.000 | 5.1 | 0.025 | 2.8 | 4.0% | 4.5% | |
C5DC | low | 0.000 | 0.4 | 0.000 | 18.0 | <0.001 | 0.4 | −5.6% | −6.2% |
medium | 0.000 | 0.7 | 0.000 | 15.2 | <0.001 | 0.7 | −4.2% | 0.9% | |
high | 0.000 | 1.8 | 0.000 | 31.7 | <0.001 | 1.7 | −10.8% | −14.2% | |
C5OH | low | 0.000 | 0.8 | 0.000 | 0.1 | 0.778 | 0.8 | 0.7% | 5.9% |
medium | 0.000 | 1.2 | 0.000 | 0.1 | 0.706 | 1.2 | −0.5% | 6.9% | |
high | 0.000 | 1.7 | 0.000 | 1.3 | 0.258 | 1.7 | 0.8% | 4.9% | |
C6 | low | 0.000 | 0.5 | 0.000 | 3.5 | 0.064 | 0.5 | 3.9% | 5.4% |
medium | 0.000 | 0.9 | 0.000 | 0.2 | 0.660 | 0.9 | 4.6% | 7.0% | |
high | 0.000 | 2.1 | 0.000 | 1.5 | 0.215 | 2.0 | 0.5% | 6.3% | |
C8 | low | 0.000 | 0.6 | 0.000 | 1.1 | 0.306 | 0.6 | −1.7% | −6.0% |
medium | 0.000 | 1.1 | 0.000 | 0.4 | 0.506 | 1.1 | 4.4% | 5.3% | |
high | 0.000 | 2.8 | 0.000 | 1.6 | 0.204 | 2.7 | −0.8% | −8.0% | |
C10 | low | 0.000 | 0.4 | 0.000 | 0.1 | 0.729 | 0.4 | 5.7% | 6.3% |
medium | 0.000 | 1.1 | 0.000 | 0.4 | 0.513 | 1.1 | 0.3% | 11.1% | |
high | 0.000 | 2.2 | 0.000 | 1.3 | 0.250 | 2.2 | −2.8% | −0.9% | |
C12 | low | 0.000 | 0.4 | 0.000 | 22.7 | <0.001 | 0.4 | 8.2% | 10.6% |
medium | 0.000 | 0.7 | 0.000 | 25.0 | <0.001 | 0.7 | 9.6% | 14.9% | |
high | 0.000 | 1.5 | 0.000 | 21.2 | <0.001 | 1.6 | 4.7% | 5.5% | |
C14 | low | 0.000 | 0.6 | 0.000 | 0.0 | 0.975 | 0.6 | −0.4% | −0.7% |
medium | 0.000 | 1.7 | 0.000 | 4.7 | 0.032 | 1.7 | 4.9% | 6.6% | |
high | 0.000 | 3.3 | 0.000 | 6.2 | 0.013 | 3.4 | 1.4% | −1.6% | |
C16 | low | 0.000 | 4.7 | 0.000 | 3.1 | 0.077 | 4.8 | 3.1% | 9.4% |
medium | 0.000 | 8.5 | 0.000 | 0.0 | 0.962 | 8.5 | 3.0% | 8.3% | |
high | 0.001 | 11.7 | 0.000 to 0.001 | 4.4 | 0.036 | 12.0 | 4.4% | 3.2% | |
C18 | low | 0.000 | 1.9 | 0.000 | 2.9 | 0.090 | 1.9 | 0.6% | −0.8% |
medium | 0.000 | 3.0 | 0.000 | 0.0 | 0.878 | 3.0 | −0.9% | 3.5% | |
high | 0.000 | 6.1 | 0.000 to 0.001 | 6.9 | 0.009 | 6.3 | 3.4% | 1.4% |
Analyte | Level | ρ | 95% Confidence Interval | p |
---|---|---|---|---|
Phe | low | 0.32 | 0.22 to 0.42 | <0.001 |
medium | 0.27 | 0.17 to 0.37 | <0.001 | |
high | 0.28 | 0.18 to 0.38 | <0.001 | |
Leu | low | 0.35 | 0.26 to 0.44 | <0.001 |
medium | 0.22 | 0.12 to 0.32 | <0.001 | |
high | 0.20 | 0.10 to 0.30 | <0.001 | |
Met | low | 0.05 | −0.05 to 0.16 | N.S |
medium | −0.08 | −0.18 to 0.03 | N.S | |
high | −0.12 | −0.23 to -0.01 | 0.027 | |
Tyr | low | 0.13 | 0.02 to 0.24 | 0.016 |
medium | 0.16 | 0.05 to 0.26 | 0.005 | |
high | 0.13 | 0.02 to 0.23 | 0.022 | |
Val | low | 0.20 | 0.10 to 0.31 | <0.001 |
medium | 0.15 | 0.04 to 0.25 | 0.007 | |
high | 0.16 | 0.05 to 0.26 | 0.004 | |
Cit | low | 0.19 | 0.08 to 0.29 | <0.001 |
medium | 0.07 | −0.04 to 0.18 | N.S | |
Arg | low | 0.26 | 0.16 to 0.36 | <0.001 |
high | −0.78 | −0.82 to -0.73 | <0.001 | |
C0 | low | 0.24 | 0.14 to 0.34 | <0.001 |
medium | 0.06 | −0.05 to 0.16 | N.S | |
high | 0.10 | −0.01 to 0.20 | N.S | |
C2 | low | −0.11 | −0.22 to 0.00 | 0.041 |
medium | −0.23 | −0.33 to -0.12 | <0.001 | |
high | −0.29 | −0.38 to -0.19 | <0.001 | |
C3 | low | 0.15 | 0.04 to 0.25 | 0.006 |
medium | −0.11 | −0.21 to 0.00 | 0.051 | |
high | −0.05 | −0.16 to 0.06 | 0.374 | |
C4 | low | −0.05 | −0.15 to 0.06 | 0.397 |
medium | −0.04 | −0.15 to 0.07 | 0.445 | |
high | −0.15 | −0.25 to -0.04 | 0.006 | |
C5 | low | 0.05 | −0.05 to 0.16 | N.S |
medium | −0.02 | −0.13 to 0.09 | N.S | |
high | 0.12 | 0.02 to 0.23 | 0.025 | |
C5DC | low | −0.23 | −0.33 to -0.12 | <0.001 |
medium | −0.21 | −0.31 to -0.11 | <0.001 | |
high | −0.30 | −0.39 to -0.20 | <0.001 | |
C5OH | low | 0.02 | −0.09 to 0.12 | N.S |
medium | −0.02 | −0.13 to 0.09 | N.S | |
high | 0.06 | −0.05 to 0.17 | N.S | |
C6 | low | −0.10 | −0.21 to 0.01 | N.S |
medium | −0.02 | −0.13 to 0.08 | N.S | |
high | −0.07 | −0.18 to 0.04 | N.S | |
C8 | low | −0.06 | −0.16 to 0.05 | N.S |
medium | 0.04 | −0.07 to 0.14 | N.S | |
high | −0.07 | −0.18 to 0.04 | N.S | |
C10 | low | 0.02 | −0.09 to 0.13 | N.S |
medium | −0.04 | −0.14 to 0.07 | N.S | |
high | −0.06 | −0.17 to 0.04 | N.S | |
C12 | low | 0.25 | 0.15 to 0.35 | < 0.001 |
medium | 0.27 | 0.16 to 0.36 | < 0.001 | |
high | 0.25 | 0.14 to 0.35 | < 0.001 | |
C14 | low | 0.00 | −0.11 to 0.11 | N.S |
medium | 0.12 | 0.01 to 0.22 | 0.032 | |
high | 0.14 | 0.03 to 0.24 | 0.013 | |
C16 | low | 0.10 | −0.01 to 0.20 | N.S |
medium | 0.00 | −0.11 to 0.11 | N.S | |
high | 0.12 | 0.01 to 0.22 | 0.036 | |
C18 | low | 0.09 | −0.01 to 0.20 | N.S |
medium | 0.01 | −0.10 to 0.12 | N.S | |
high | 0.14 | 0.04 to 0.25 | 0.009 |
Study | This Study | Adam et al. | Golbahar et al. |
---|---|---|---|
Publication year | 2021 | 2011 | 2014 |
Method | real-time monitoring | accelerated degradation study | accelerated degradation study |
Analytes | 7 AAs, 14 ACs | 7 AAs, 18 ACs, 8 hormones and metabolites | 7AAs, 10 ACs |
Study length | 778 days | 35 days | 8 days |
Compared temperature | −20 °C | 37 °C | 37 °C |
Compared humidity | <30% | >50% | >70% |
Compared analytes | 7 AAs, 9 ACs | ||
most sensitive analytes | Arg, Phe, Leu | Arg, C2, C3 | Met, Tyr, Arg |
least sensitive analytes | C5OH, C6, C8, C10 | Val, C5, C16, C18 | C0, C5, Phe |
Reference | [17] | [20] |
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Lee, H.-S. Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot. Separations 2021, 8, 201. https://doi.org/10.3390/separations8110201
Lee H-S. Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot. Separations. 2021; 8(11):201. https://doi.org/10.3390/separations8110201
Chicago/Turabian StyleLee, Hyun-Seung. 2021. "Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot" Separations 8, no. 11: 201. https://doi.org/10.3390/separations8110201
APA StyleLee, H. -S. (2021). Arginine, as a Key Indicator for Real-Time Stability Monitoring of Quality Control in the Newborn Screening Test Using Dried Blood Spot. Separations, 8(11), 201. https://doi.org/10.3390/separations8110201