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Separations 2018, 5(2), 27; https://doi.org/10.3390/separations5020027

Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF)

1
Department of Chemistry-BMC, Analytical Chemistry, Uppsala University, P.O. Box 599, SE-751 24 Uppsala, Sweden
2
Department of Engineering Sciences, Uppsala University, PO Box 534, SE-751 21 Uppsala, Sweden
*
Author to whom correspondence should be addressed.
Received: 16 February 2018 / Revised: 23 March 2018 / Accepted: 26 April 2018 / Published: 7 May 2018
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Abstract

In this paper, a strategy for structured monitoring of surface modifications to control protein adsorption to membrane structures is presented. The already established on-surface enzymatic digestion (oSED) method combined with nano-liquid chromatography and tandem mass spectrometry (LC-MS/MS) analysis was employed for the analysis of proteins in ventricular cerebrospinal fluid (vCSF) from neurointensive care patients. Protein adsorption was studied by in-situ sampling in a temporally resolved manner on both immobilized native and Pluronic-deactivated membranes. Deactivation was significantly reducing the protein adsorption but it also induced novel selective properties of the surface. The proposed versatile strategy will facilitate protein-biomaterial, protein-polymer, protein-protein interaction studies in the future. View Full-Text
Keywords: protein adsorption; surface modification; Pluronic F-127; LC MS/MS protein adsorption; surface modification; Pluronic F-127; LC MS/MS
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Undin, T.; P. Dahlin, A.; Bergquist, J.; Bergström Lind, S. Mass Spectrometric Determination of the Effect of Surface Deactivation on Membranes Used for In-Situ Sampling of Cerebrospinal Fluid (CSF). Separations 2018, 5, 27.

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