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Separations 2018, 5(1), 17; https://doi.org/10.3390/separations5010017

Separation and Determination of Some of the Main Cholesterol-Related Compounds in Blood by Gas Chromatography-Mass Spectrometry (Selected Ion Monitoring Mode)

1
Department of Analytical Chemistry, Faculty of Science, University of Granada, C/Fuentenueva s/n, E-18071 Granada, Spain
2
Neuron Bio, P.T.S. Granada, C/Avicena 4, E-18016 Granada, Spain
*
Author to whom correspondence should be addressed.
Received: 22 September 2017 / Revised: 12 February 2018 / Accepted: 27 February 2018 / Published: 5 March 2018
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Abstract

Oxysterols are metabolites produced in the first step of cholesterol metabolism, which is related to neurodegenerative disorder. They can be detected by testing blood, plasma, serum, or cerebrospinal fluid. In this study, some cholesterol precursors and oxysterols were determined by gas chromatography coupled to mass spectrometry. The selected cholesterol-related compounds were desmosterol, lathosterol, lanosterol, 7α-hydroxycholesterol, 7β-hydroxycholesterol, 24(S)-hydroxycholesterol, 25-hydroxycholesterol, 7-ketocholesterol, and 27-hydroxycholesterol. A powerful method was developed and validated considering various analytical parameters, such as linearity index, detection and quantification limits, selectivity and matrix effect, precision (repeatability), and trueness (recovery factor) for each cholesterol-related compound. 7α-hydroxycholesterol, 7β-hydroxycholesterol, and desmosterol exhibited the lowest detection and quantification limits, with 0.01 and 0.03 μg/mL, respectively, in the three cases. 7-ketocholesterol and lathosterol showed matrix effect percentages between 95.5% and 104.8%, respectively (demonstrating a negligible matrix effect), and very satisfactory repeatability values (i.e., overall performance of the method). Next, the method was applied to the analysis of a very interesting selection of mouse plasma samples (9 plasma extracts of non-transgenic and transgenic mice that had been fed different diets). Although the number of samples was limited, the current study led to some biologically relevant conclusions regarding brain cholesterol metabolism. View Full-Text
Keywords: cholesterol-related compounds; GC-(IT)MS; selected ion monitoring mode cholesterol-related compounds; GC-(IT)MS; selected ion monitoring mode
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Valverde-Som, L.; Carrasco-Pancorbo, A.; Sierra, S.; Santana, S.; Ruiz-Samblás, C.; Navas, N.; Burgos, J.S.; Cuadros-Rodríguez, L. Separation and Determination of Some of the Main Cholesterol-Related Compounds in Blood by Gas Chromatography-Mass Spectrometry (Selected Ion Monitoring Mode). Separations 2018, 5, 17.

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