Determination of 2-Thioxo-3-pyrrolidinecarbaldehyde in Salted Radish Root (Takuan-zuke) by High-Performance Liquid Chromatography with Fluorescence Detection after Pre-Column Derivatization Using 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials and Reagents
2.2. Fluorescent Labeling with DBD-H
2.2.1. TPC Standard Solution
2.2.2. Takuan-zuke Sample
2.3. Analysis of TPC and MTB-ITC Contained in Daikon-oroshi (Grated Radish)
2.3.1. Preparation of Grated Radish Juice
2.3.2. Fluorescent Labeling of TPC from Grated Radish Juice with DBD-H
2.3.3. Gas Chromatography Analysis of MTB-ITC in Grated Radish Juice
2.4. HPLC System and Conditions
2.5. GC System and Conditions
3. Results and Discussion
3.1. Effects of Acetonitrile Concentration and Reaction Time on Extraction and Reaction Efficiency
3.2. Effects of Trifluoroacetic Acid Concentration on Reaction Efficiency of DBD-H and Stability of DBD-TPC
3.3. Effect of pH on Stability of DBD-TPC
3.4. Verification of Sensitivity
3.5. Quantification of TPC and MTB-ITC in Grated Radish Juice
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Kobayashi, T.; Kumakura, K.; Kobayashi, W.; Takahashi, A.; Matsuoka, H. Determination of 2-Thioxo-3-pyrrolidinecarbaldehyde in Salted Radish Root (Takuan-zuke) by High-Performance Liquid Chromatography with Fluorescence Detection after Pre-Column Derivatization Using 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole. Separations 2017, 4, 35. https://doi.org/10.3390/separations4040035
Kobayashi T, Kumakura K, Kobayashi W, Takahashi A, Matsuoka H. Determination of 2-Thioxo-3-pyrrolidinecarbaldehyde in Salted Radish Root (Takuan-zuke) by High-Performance Liquid Chromatography with Fluorescence Detection after Pre-Column Derivatization Using 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole. Separations. 2017; 4(4):35. https://doi.org/10.3390/separations4040035
Chicago/Turabian StyleKobayashi, Taito, Kei Kumakura, Wataru Kobayashi, Asaka Takahashi, and Hiroki Matsuoka. 2017. "Determination of 2-Thioxo-3-pyrrolidinecarbaldehyde in Salted Radish Root (Takuan-zuke) by High-Performance Liquid Chromatography with Fluorescence Detection after Pre-Column Derivatization Using 4-(N,N-dimethylaminosulfonyl)-7-hydrazino-2,1,3-benzoxadiazole" Separations 4, no. 4: 35. https://doi.org/10.3390/separations4040035