Next Article in Journal
GC-MS-Olfactometric Characterization of Volatile and Key Odorants in Moringa (Moringa oleifera) and Kinkeliba (Combretum micranthum G. Don) Herbal Tea Infusions Prepared from Cold and Hot Brewing
Next Article in Special Issue
A Comparative Study of Chamomile Essential Oils and Lipophilic Extracts Obtained by Conventional and Greener Extraction Techniques: Chemometric Approach to Chemical Composition and Biological Activity
Previous Article in Journal
Purification of Carrier-Free 47Sc of Biomedical Interest: Selective Separation Study from natCa(n,γ)
Previous Article in Special Issue
Development of an HPLC-DAD Method for the Extraction and Quantification of 5-Fluorouracil, Uracil, and 5-Fluorodeoxyuridin Monophosphate in Cells and Culture Media of Lactococcus lactis
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Separations
Volume 10
Issue 1
10.3390/separations10010009
separations-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography

by
Laura Toribio
,
Irene Magdaleno
,
Beatriz Martín-Gómez
,
María T. Martín
,
Silvia Valverde
and
Ana M. Ares
*
I. U. CINQUIMA, Analytical Chemistry Group (TESEA), Faculty of Sciences, University of Valladolid, 47011 Valladolid, Spain
*
Author to whom correspondence should be addressed.
Separations 2023, 10(1), 9; https://doi.org/10.3390/separations10010009
Submission received: 18 November 2022 / Revised: 12 December 2022 / Accepted: 20 December 2022 / Published: 23 December 2022
(This article belongs to the Special Issue Women in Separations)
Browse Figures
Versions Notes

Abstract

The enantiomeric separation of antifungal compounds is an arduous task in pharmaceutical and biomedical fields due to the different properties that each diastereoisomer presents. The enantioseparation of a group of fungicides (sulconazole, bifonazole, triadimefon and triadimenol) using supercritical fluid chromatography was achieved in this work. For this goal, four different chiral columns based on polysaccharide derivatives, as well as the effect of different chromatographic parameters such as temperature, type and percentage of organic modifier (methanol, ethanol and isopropanol), were thoroughly investigated. The inversion of the elution order of enantiomers as a result of a change in the stationary phase or organic modifier was also evaluated by employing a circular dichroism detector. The best separation conditions, in terms of the enantioresolution and analysis time, were obtained with the Lux® Cellulose-2 column using isopropanol as the organic modifier.
Keywords: enantiomeric separation; supercritical fluid chromatography; antifungal compounds; polysaccharide chiral columns; elution order; azoles; SFC separation enantiomeric separation; supercritical fluid chromatography; antifungal compounds; polysaccharide chiral columns; elution order; azoles; SFC separation
Graphical Abstract

Share and Cite

MDPI and ACS Style

Toribio, L.; Magdaleno, I.; Martín-Gómez, B.; Martín, M.T.; Valverde, S.; Ares, A.M. Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography. Separations 2023, 10, 9. https://doi.org/10.3390/separations10010009

AMA Style

Toribio L, Magdaleno I, Martín-Gómez B, Martín MT, Valverde S, Ares AM. Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography. Separations. 2023; 10(1):9. https://doi.org/10.3390/separations10010009

Chicago/Turabian Style

Toribio, Laura, Irene Magdaleno, Beatriz Martín-Gómez, María T. Martín, Silvia Valverde, and Ana M. Ares. 2023. "Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography" Separations 10, no. 1: 9. https://doi.org/10.3390/separations10010009

APA Style

Toribio, L., Magdaleno, I., Martín-Gómez, B., Martín, M. T., Valverde, S., & Ares, A. M. (2023). Study of Different Chiral Columns for the Enantiomeric Separation of Azoles Using Supercritical Fluid Chromatography. Separations, 10(1), 9. https://doi.org/10.3390/separations10010009

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop