Protective Effect of Salvianolic Acid B in Acetic Acid-Induced Experimental Colitis in a Mouse Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Animals and Ethical Approval
2.3. Induction of Experimental Colitis in Mice
2.4. Animals
2.5. Macroscopic Damage Score
- 0 = No damage
- 1 = localized hyperemia but no ulcers
- 2 = linear ulcers with no significant inflammation
- 3 = linear ulcers with inflammation at one site
- 4 = two or more sites of ulceration & inflammation
- 5 = two or more sites of ulceration with major inflammation >1 cm along the length of the colon.
2.6. Histopathological Evaluation of Colitis
2.7. Biochemical Measurements
2.8. Measurement of CAT Activity in Colon Tissues
2.9. Measurement of SOD Activity in Colon Tissues
2.10. Measurement of GSH Activity in Colon Tissues
2.11. Measurement of LPO Activity in Colon Tissues
2.12. Measurement of MPO Activity in Colon Tissues
2.13. Determination of Aberrant Crypt Foci (ACF)
2.14. Mast Cell Staining on Colon of Experimental Animals
2.15. Statistical Analysis
3. Results
3.1. Effect of Sal B on AA-Induced Colitis
3.2. Sal B Decreases Microscopic Colon Damage in AA-Induced Experimental Colitis
3.3. Effect of Sal B on CAT Activity in Colon Tissues
3.4. Effect of Sal B on SOD Activity in Colon Tissues
3.5. Effect of Sal B on GSH Activity in Colon Tissues
3.6. Effect of Sal B on MDA Levels in Colon Tissues
3.7. Effect of Sal B on Myeloperoxidase Activity in Colon Tissues
3.8. Methylene Blue Staining of Aberrant Crypt Foci (ACF) of Experimental Mice
3.9. Mast Cell Count of Control and Experimental Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Ethics Approval
References
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Parameters/Groups | Colon Catalase Activity (µmol H2O2/min/mg Protein) | Colon SOD Activity (U/mg Protein) | Colon GSH Activity (nmol/mg Protein) |
---|---|---|---|
Control (Saline) | 9.15 ± 0.489 | 8.74 ± 0.53 | 10.74 ± 0.72 |
Colitis (AA) | 2.72 ± 0.213 | 2.31 ± 0.19 | 3.36 ± 0.23 |
Colitis + 10 µg Sal B | 6.47 ± 0.331 | 7.78 ± 0.45 | 4.99 ± 0.39 |
Colitis + 20 µg Sal B | 5.99 ± 0.361 | 6.61 ± 0.35 | 5.41 ± 0.43 |
Colitis + 40 µg Sal B | 7.22 ± 0.443 | 6.78 ± 0.39 | 7.74 ± 0.56 |
Parameters/Groups | Colon MDA Levels (nmol/mg Protein) | Colon MPO Activity (U/mg Tissue) |
---|---|---|
Control | 18.02 ± 1.87 | 1.25 ± 0.14 |
Colitis | 45.29 ± 2.34 | 18.42 ± 0.98 |
Colitis+ 10 µg Sal B | 23.34 ±1.83 | 6.43 ± 0.45 |
Colitis+ 20 µg Sal B | 15.21 ± 1.24 | 9.28 ± 0.72 |
Colitis+ 40 µg Sal B | 10.44 ± 0.91 | 4.58 ± 0.36 |
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Govindarasu, M.; Ansari, M.A.; Alomary, M.N.; AlYahya, S.; Alghamdi, S.; Bannunah, A.M.; Almehmadi, M.; Abirami, P.; Gayathiri, E.; Palani, M.; et al. Protective Effect of Salvianolic Acid B in Acetic Acid-Induced Experimental Colitis in a Mouse Model. Processes 2021, 9, 1589. https://doi.org/10.3390/pr9091589
Govindarasu M, Ansari MA, Alomary MN, AlYahya S, Alghamdi S, Bannunah AM, Almehmadi M, Abirami P, Gayathiri E, Palani M, et al. Protective Effect of Salvianolic Acid B in Acetic Acid-Induced Experimental Colitis in a Mouse Model. Processes. 2021; 9(9):1589. https://doi.org/10.3390/pr9091589
Chicago/Turabian StyleGovindarasu, Mydhili, Mohammad Azam Ansari, Mohammad N. Alomary, Sami AlYahya, Saad Alghamdi, Azzah M. Bannunah, Mazen Almehmadi, Pari Abirami, Ekambaram Gayathiri, Mariyappan Palani, and et al. 2021. "Protective Effect of Salvianolic Acid B in Acetic Acid-Induced Experimental Colitis in a Mouse Model" Processes 9, no. 9: 1589. https://doi.org/10.3390/pr9091589