Antioxidant Activity of Spiranthes sinensis and Its Protective Effect against UVB-Induced Skin Fibroblast Damage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Extraction of S. sinensis
2.2. Determination of Antioxidants Content
2.2.1. Total Phenols
2.2.2. Total Flavonoid Content
2.2.3. Condensed Tannins
2.3. Evaluation of Antioxidant Activity
2.3.1. DPPH Assay of Free Radical Scavenging Activity
2.3.2. ABTS Assay of Free Radical Scavenging Activity
2.3.3. Ferric Reducing Ability of Plasma (FRAP)
2.3.4. Ferrous Ion (Fe2+) Chelating Activity
2.3.5. Oxygen Radical Absorbance Capacity (ORAC)
2.4. Cell Line and Cell Culture
2.5. MTT Cell Viability Assay and Cytotoxicity of S. sinensis Extracts
2.6. Protective Effects of S. sinensis Extracts against UVB
2.7. Determination of Intracellular GSH
2.8. Statistical Analysis
3. Results and Discussion
3.1. Extraction Yields and Antioxidants Content in Different Extracts
3.2. Radical Scavenging Activity
3.3. Ferric Reducing Antioxidant Power (FRAP)
3.4. Ferrous Ion-Chelating Activity
3.5. Oxygen Radical Absorbance Capacity
3.6. Cytotoxicity of the Extracts in Human Fibroblasts
3.7. Effect of UVB on the Viability of Human Fibroblasts
3.8. Effect of the Extracts on the Viability of Human Fibroblasts Exposed to UVB
3.9. Effect of the Extracts on the GSH Content of Human Fibroblasts Exposed to UVB
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Extraction Solvent | Extraction Yield (%) 1,2 | Content 1 | ||
---|---|---|---|---|
Total Phenols (mg GAE 3/g Extract) | Total Flavonoids (mg QE 4/g Extract) | Condensed Tannins (mg CE 5/g Extract) | ||
water | 25.11 ± 2.83 b | 95.12 ± 0.24 b | 7.77 ± 0.40 b | 194.41 ± 12.84 b |
75% ethanol | 13.50 ± 2.21 a | 161.86 ± 1.23 a | 15.89 ± 1.60 a | 439.11 ± 16.78 a |
Radical Scavenging EC50 1,2 (μg/mL) | FRAP 1,3 (μmol Fe2+/g Extract) | Metal Chelating 1 (μmol EDTAE 4/g Extract) | ORAC 1,5 (μmol TE 6/g Extract) | ||
---|---|---|---|---|---|
DPPH | ABTS | ||||
Water extract | 659.38 ± 9.30 a | 5167.33 ± 207.81 a | 15.14 ± 0.34 d | 25.13 ± 0.60 d | 683.38 ± 131.64 d |
Ethanol extract | 446.90 ± 5.23 b | 3031.15 ± 105.92 b | 38.43 ± 0.80 c | 38.04 ± 0.40 c | 2010.69 ± 106.58 c |
Ascorbic acid | 42.52 ± 0.24 c | 451.54 ± 8.63 c | 968.08 ± 8.54 b | 54.62 ± 0.71 b | 2556.49 ± 127.76 b |
Quercetin | 19.72 ± 1.44 d | 126.84 ± 3.42 d | 1356.75 ± 58.69 a | 174.63 ± 4.03 a | 3187.04 ± 100.44 a |
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Huang, S.-M.; Shieh, C.-J.; Wu, Y.-L.; Pan, Y.-Z.; Yu, C.-Y. Antioxidant Activity of Spiranthes sinensis and Its Protective Effect against UVB-Induced Skin Fibroblast Damage. Processes 2021, 9, 1564. https://doi.org/10.3390/pr9091564
Huang S-M, Shieh C-J, Wu Y-L, Pan Y-Z, Yu C-Y. Antioxidant Activity of Spiranthes sinensis and Its Protective Effect against UVB-Induced Skin Fibroblast Damage. Processes. 2021; 9(9):1564. https://doi.org/10.3390/pr9091564
Chicago/Turabian StyleHuang, Shang-Ming, Chwen-Jen Shieh, Yu-Lin Wu, Yang-Zing Pan, and Chi-Yang Yu. 2021. "Antioxidant Activity of Spiranthes sinensis and Its Protective Effect against UVB-Induced Skin Fibroblast Damage" Processes 9, no. 9: 1564. https://doi.org/10.3390/pr9091564