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Open AccessFeature PaperArticle

Single-Use Printed Biosensor for L-Lactate and Its Application in Bioprocess Monitoring

1
Department of Printed Electronics, RISE Acreo, Research Institute of Sweden, 60221 Norrköping, Sweden
2
Division of Biotechnology, IFM, Linköping University, 58183 Linköping, Sweden
3
Department of Bioprocess Engineering, Technische Universität Berlin, 13355 Berlin, Germany
*
Author to whom correspondence should be addressed.
Shared first authors.
Processes 2020, 8(3), 321; https://doi.org/10.3390/pr8030321
Received: 12 February 2020 / Revised: 27 February 2020 / Accepted: 4 March 2020 / Published: 9 March 2020
(This article belongs to the Special Issue Measurement Technologies for Up- and Downstream Bioprocessing)
There is a profound need in bioprocess manufacturing for low-cost single-use sensors that allow timely monitoring of critical product and production attributes. One such opportunity is screen-printed enzyme-based electrochemical sensors, which have the potential to enable low-cost online and/or off-line monitoring of specific parameters in bioprocesses. In this study, such a single-use electrochemical biosensor for lactate monitoring is designed and evaluated. Several aspects of its fabrication and use are addressed, including enzyme immobilization, stability, shelf-life and reproducibility. Applicability of the biosensor to off-line monitoring of bioprocesses was shown by testing in two common industrial bioprocesses in which lactate is a critical quality attribute (Corynebacterium fermentation and mammalian Chinese hamster ovary (CHO) cell cultivation). The specific response to lactate of the screen-printed biosensor was characterized by amperometric measurements. The usability of the sensor at typical industrial culture conditions was favorably evaluated and benchmarked with commonly used standard methods (HPLC and enzymatic kits). The single-use biosensor allowed fast and accurate detection of lactate in prediluted culture media used in industrial practice. The design and fabrication of the biosensor could most likely be adapted to several other critical bioprocess analytes using other specific enzymes. This makes this single-use screen-printed biosensor concept a potentially interesting and versatile tool for further applications in bioprocess monitoring. View Full-Text
Keywords: lactate biosensor; enzyme electrode; off-line monitoring; screen-printing; at-line measurement; in-line monitoring lactate biosensor; enzyme electrode; off-line monitoring; screen-printing; at-line measurement; in-line monitoring
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MDPI and ACS Style

Theuer, L.; Randek, J.; Junne, S.; Neubauer, P.; Mandenius, C.-F.; Beni, V. Single-Use Printed Biosensor for L-Lactate and Its Application in Bioprocess Monitoring. Processes 2020, 8, 321.

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