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Volume 11
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10.3390/pr11010028
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Article

The Effect of Low Positive Temperatures on the Formation of Secondary Metabolites in Rhodiola quadrifida (Pall.) Fisch. et C.A. Mey. In Vitro Cultures

by
Anna Y. Stepanova
1,*,
Evgeny A. Gladkov
1,*,
Dmitry V. Tereshonok
1,
Renat N. Selimov
2,
Elisaveta N. Goncharova
2 and
Aleksandra I. Solov’eva
1
1
K.A. Timiryazev Institute of Plant Physiology Russian Academy of Sciences, 127276 Moscow, Russia
2
Bioanalytical Laboratory OOO “NVC Agrovetzaschita”, 129329 Moscow, Russia
*
Authors to whom correspondence should be addressed.
Processes 2023, 11(1), 28; https://doi.org/10.3390/pr11010028
Submission received: 24 November 2022 / Revised: 13 December 2022 / Accepted: 19 December 2022 / Published: 23 December 2022
(This article belongs to the Section Pharmaceutical Processes)
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Abstract

Global warming is one of the most serious problems leading to changes in the distribution areas of species and biodiversity. Rhodiola quadrifida is a rare plant with adaptogenic properties and grows in the highlands in a narrow temperature range of 2–15 °C. The aim of our work was to study the growth and content of the main metabolites in two in vitro cultures of Rhodiola quadrifida at temperatures of 5, 15 and 25 °C. Hairy roots and calli were cultivated on agar medium for 28 days. The maximum values of the growth index were observed at 25 °C (2.32 and 2.12 for calli and hairy roots, respectively). HPLC-MS showed the absence of tyrosol and rosarin in both cultures, and rosin in the root culture. The content of salidroside changed slightly in calli and roots. Cultivation at 5 °C significantly stimulated the formation of rosin in calli. Only a residual amount of rosavin was noted in the roots, regardless of temperature. The content of rosin was higher in calli at 15 °C with a maximum content at the end of the cultivation cycle 25 µg/g DW. Thus, Rhodiola quadrifida will be able to grow with an increase in temperature by 10 °C but this will be accompanied by a significant reduction in its medicinal value
Keywords: Rhodiola quadrifida; callus; hairy roots; salidroside; rosavin; rosarin; rosin; tyrosol Rhodiola quadrifida; callus; hairy roots; salidroside; rosavin; rosarin; rosin; tyrosol

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MDPI and ACS Style

Stepanova, A.Y.; Gladkov, E.A.; Tereshonok, D.V.; Selimov, R.N.; Goncharova, E.N.; Solov’eva, A.I. The Effect of Low Positive Temperatures on the Formation of Secondary Metabolites in Rhodiola quadrifida (Pall.) Fisch. et C.A. Mey. In Vitro Cultures. Processes 2023, 11, 28. https://doi.org/10.3390/pr11010028

AMA Style

Stepanova AY, Gladkov EA, Tereshonok DV, Selimov RN, Goncharova EN, Solov’eva AI. The Effect of Low Positive Temperatures on the Formation of Secondary Metabolites in Rhodiola quadrifida (Pall.) Fisch. et C.A. Mey. In Vitro Cultures. Processes. 2023; 11(1):28. https://doi.org/10.3390/pr11010028

Chicago/Turabian Style

Stepanova, Anna Y., Evgeny A. Gladkov, Dmitry V. Tereshonok, Renat N. Selimov, Elisaveta N. Goncharova, and Aleksandra I. Solov’eva. 2023. "The Effect of Low Positive Temperatures on the Formation of Secondary Metabolites in Rhodiola quadrifida (Pall.) Fisch. et C.A. Mey. In Vitro Cultures" Processes 11, no. 1: 28. https://doi.org/10.3390/pr11010028

APA Style

Stepanova, A. Y., Gladkov, E. A., Tereshonok, D. V., Selimov, R. N., Goncharova, E. N., & Solov’eva, A. I. (2023). The Effect of Low Positive Temperatures on the Formation of Secondary Metabolites in Rhodiola quadrifida (Pall.) Fisch. et C.A. Mey. In Vitro Cultures. Processes, 11(1), 28. https://doi.org/10.3390/pr11010028

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