Assessment of Platelet Mitochondrial Respiration in a Pediatric Population: A Pilot Study in Healthy Children and Children with Acute Lymphoblastic Leukemia

Lelcu, Theia; Bînă, Anca M.; Dănilă, Maria D.; Popoiu, Călin M.; Aburel, Oana M.; Arghirescu, Smaranda T.; Borza, Claudia; Muntean, Danina M.

doi:10.3390/children8121196

Open AccessArticle

Assessment of Platelet Mitochondrial Respiration in a Pediatric Population: A Pilot Study in Healthy Children and Children with Acute Lymphoblastic Leukemia

by

Theia Lelcu

^1,2,†

,

Anca M. Bînă

^1,2,†,

Maria D. Dănilă

^1,2,

Călin M. Popoiu

^3,4,

Oana M. Aburel

^1,2,

Smaranda T. Arghirescu

^4,5,*,

Claudia Borza

^1,2,* and

Danina M. Muntean

^1,2

¹

Department III Functional Sciences, Discipline Pathophysiology, “Victor Babeș” University of Medicine and Pharmacy, E. Murgu Sq. No. 2, 300041 Timișoara, Romania

²

Centre for Translational Research and Systems Medicine, “Victor Babeș” University of Medicine and Pharmacy, E. Murgu Sq. No. 2, 300041 Timișoara, Romania

³

Department XI Pediatrics, Discipline Pediatric Surgery, “Victor Babeș” University of Medicine and Pharmacy, E. Murgu Sq. No. 2, 300041 Timișoara, Romania

⁴

Department XI Pediatrics, Discipline Pediatrics III, “Victor Babeș” University of Medicine and Pharmacy, E. Murgu Sq. No. 2, 300041 Timișoara, Romania

⁵

“Louis Țurcanu” Emergency Hospital for Children, 300011 Timișoara, Romania

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Children 2021, 8(12), 1196; https://doi.org/10.3390/children8121196

Submission received: 1 November 2021 / Revised: 11 December 2021 / Accepted: 13 December 2021 / Published: 17 December 2021

(This article belongs to the Special Issue Current Status of Cancer Research in Adolescents—Problems of Children with Cancer)

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Abstract

Characterization of mitochondrial respiration in peripheral blood cells has recently emerged as a potential biomarker for the assessment of the severity of hematological malignancies (HM) in adults. Whether changes in platelet respiratory function occur in children with or without HM it is unknown. The present pilot study was double-aimed: (i) to investigate whether platelet respiration is age-dependent in non-HM children and (ii) to assess the platelet mitochondrial respiration in children with newly diagnosed acute lymphoblastic leukemia (ALL). Blood samples obtained from age-grouped children (10–11, 13–14 and 16–17 years) with non-HM and children with ALL (10–11 years) were used to isolate platelets via differential centrifugation. High-resolution respirometry studies of isolated platelets were performed according to a protocol adapted to evaluate complex I and II-supported respiration. An age-related decrease in respiration was observed in the non-HM pediatric population and had comparable values for the 13–14 and 16–17 years. groups. In children with ALL, a significant increase in C I-supported active respiration and decrease in maximal noncoupled respiration were found at the disease onset. In conclusion, in a pediatric population, platelet mitochondrial respiration is age-dependent. Platelet respiratory dysfunction occurs in children with newly-diagnosed ALL, an observation that warrants further investigation of this change as a disease biomarker.

Keywords: platelets; high-resolution respirometry; children; age-dependency of mitochondrial respiration; acute lymphoblastic leukemia

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MDPI and ACS Style

Lelcu, T.; Bînă, A.M.; Dănilă, M.D.; Popoiu, C.M.; Aburel, O.M.; Arghirescu, S.T.; Borza, C.; Muntean, D.M. Assessment of Platelet Mitochondrial Respiration in a Pediatric Population: A Pilot Study in Healthy Children and Children with Acute Lymphoblastic Leukemia. Children 2021, 8, 1196. https://doi.org/10.3390/children8121196

AMA Style

Lelcu T, Bînă AM, Dănilă MD, Popoiu CM, Aburel OM, Arghirescu ST, Borza C, Muntean DM. Assessment of Platelet Mitochondrial Respiration in a Pediatric Population: A Pilot Study in Healthy Children and Children with Acute Lymphoblastic Leukemia. Children. 2021; 8(12):1196. https://doi.org/10.3390/children8121196

Chicago/Turabian Style

Lelcu, Theia, Anca M. Bînă, Maria D. Dănilă, Călin M. Popoiu, Oana M. Aburel, Smaranda T. Arghirescu, Claudia Borza, and Danina M. Muntean. 2021. "Assessment of Platelet Mitochondrial Respiration in a Pediatric Population: A Pilot Study in Healthy Children and Children with Acute Lymphoblastic Leukemia" Children 8, no. 12: 1196. https://doi.org/10.3390/children8121196

APA Style

Lelcu, T., Bînă, A. M., Dănilă, M. D., Popoiu, C. M., Aburel, O. M., Arghirescu, S. T., Borza, C., & Muntean, D. M. (2021). Assessment of Platelet Mitochondrial Respiration in a Pediatric Population: A Pilot Study in Healthy Children and Children with Acute Lymphoblastic Leukemia. Children, 8(12), 1196. https://doi.org/10.3390/children8121196

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Assessment of Platelet Mitochondrial Respiration in a Pediatric Population: A Pilot Study in Healthy Children and Children with Acute Lymphoblastic Leukemia

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