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Article

Dendritic Spine in Autism Genetics: Whole-Exome Sequencing Identifying De Novo Variant of CTTNBP2 in a Quad Family Affected by Autism Spectrum Disorder

by
Yingmei Xie
1,†,
Hui Wang
2,†,
Bing Hu
1,†,
Xueli Zhang
3,
Aiping Liu
4,
Chunquan Cai
5,
Shijun Li
6,
Cheng Chen
1,
Zhangxing Wang
3,
Zhaoqing Yin
7,* and
Mingbang Wang
8,9,*
1
Division of Neonatology, Longgang District Maternal and Child Health Hospital, Shenzhen 518172, China
2
Division of Child Health Care, Xiamen Branch of Children’s Hospital of Fudan University (Xiamen Children’s Hospital), Xiamen 361006, China
3
Division of Neonatology, Shenzhen Longhua People’s Hospital, Shenzhen 518109, China
4
The Department of Laboratory, Baoan Public Health Service Center of Shenzhen, Shenzhen 518108, China
5
Tianjin Key Laboratory of Birth Defects for Prevention and Treatment, Tianjin Children’s Hospital (Children’s Hospital of Tianjin University), Tianjin Pediatric Research Institute, Tianjin 300134, China
6
First Medical Center, Chinese PLA General Hospital, Department of Radiology, Beijing 100853, China
7
The People’s Hospital of Dehong Autonomous Prefecture, Division of Pediatrics, Dehong Hospital of Kun-ming Medical University, Mangshi 678400, China
8
Microbiome Therapy Center, South China Hospital, Medical School, Shenzhen University, Shenzhen 518116, China
9
Shanghai Key Laboratory of Birth Defects, Division of Neonatology, Children’s Hospital of Fudan University, National Center for Children’s Health, Shanghai 201102, China
*
Authors to whom correspondence should be addressed.
†
These authors are contributed equally to this work.
Children 2023, 10(1), 80; https://doi.org/10.3390/children10010080
Submission received: 4 November 2022 / Revised: 5 December 2022 / Accepted: 24 December 2022 / Published: 30 December 2022
(This article belongs to the Special Issue Human Genetics and Bioinformatics in Pediatric Diseases)
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Abstract

:
Autism spectrum disorder (ASD) affects around 1% of children with no effective blood test or cure. Recent studies have suggested that these are neurological disorders with a strong genetic basis and that they are associated with the abnormal formation of dendritic spines. Chromosome microarray (CMA) together with high-throughput sequencing technology has been used as a powerful tool to identify new candidate genes for ASD. In the present study, CMA was first used to scan for genome-wide copy number variants in a proband, and no clinically significant copy number variants were found. Whole-exome sequencing (WES) was used further for genetic testing of the whole quad family affected by ASD, including the proband, his non-autistic sister, and his parents. Sanger sequencing and MassARRAY-based validation were used to identify and confirm variants associated with ASD. WES yielded a 151-fold coverage depth for each sample. A total of 98.65% of the targeted whole-exome region was covered at >20-fold depth. A de novo variant in CTTNBP2, p.M115T, was identified. The CTTNBP2 gene belongs to a family of ankyrin repeat domain-containing proteins associated with dendritic spine formation. Although CTTNBP2 has been associated with ASD, limited studies have been developed to identify clinically relevant de novo mutations of CTTNBP2 in children with ASD; family-based WES successfully identified a clinically relevant mutation in the CTTNBP2 gene in a quad family affected by ASD. Considering the neuron-specific expression of CTTNBP2 and its role in dendritic spine formation, our results suggest a correlation between the CTTNBP2 mutation and ASD, providing genetic evidence for ASD spine pathology. Although the present study is currently insufficient to support the assertion that the de novo mutation M115T in CTTNBP2 directly causes the autism phenotype, our study provides support for the assertion that this mutation is a candidate clinically relevant variant in autism.

1. Introduction

Autism spectrum disorder (ASD), a group of lifelong neurodevelopmental disorders, affects more than 78 million people worldwide and identifying the etiology of autism and developing targeted pharmacological treatment options is a priority [1,2]. Genetic background is thought to play an increasingly important role in the development of autism, and the evidence supporting this assertion comes primarily from studies of twins, which show that identical twins are significantly more likely to share autism than dizygotic twins; a 50–80% heritability of autism has been found [3,4,5,6]. The prevalence of autism is gradually increasing, and the arguments in support of this fact come primarily from epidemiological survey data from the Centers for Disease Control and Prevention (CDC) in the US. The CDC data indicate that the prevalence of ASD has increased from an estimated 1 in 166 in 2004 to 1 in 44 in 2018, or almost tripled [7]. This observation also challenges the classical hypothesis of the genetic etiology of ASD. Notably, the contribution of environmental factors to ASD has also been increasingly noted [6]. As opposed to genetic factors, the role of environmental factors in ASD appears to be more complex. New studies suggest that environmental factors may play a role in autism development by interacting with genes. For example, environmental factors may directly influence the production of de novo mutations or affect DNA methylation [8,9].
The widespread use of large-scale high-throughput sequencing in autism research [10,11,12,13,14] has directly led to the discovery of a large number of autism-causing genes or related genes, and, as of the date of writing of the manuscript (1 November 2022), as many as 1095 autism-causing genes or related genes were included in the SFARI Gene database (https://gene.sfari.org/database/gene-scoring/ (accessed on 1 November 2022)). Notably, in addition to rare variants inherited from parents, germline de novo variants and chimeric mutations contribute significantly to autism [15]. However, the discovery of a large number of autism genes has been accompanied by the identification of a few genes with high-frequency mutations in autistic families, which has made it difficult to carry out mechanistic studies in genetically modified animal models of autism [16]; encouragingly, patient-derived induced pluripotent stem cells or organoid-based studies are beginning to reveal commonalities in the etiology of autism on different genetic mutation backgrounds [17]. In the meantime, transcriptional-level studies of autism and biological pathway enrichment analyses have revealed a number of genes that control synapse formation and signaling as well as genes that control transcription and chromatin conformation to regulate gene expression and play a key role in autism [18,19,20,21].
Genes that control synapse formation and synaptic signaling activity are an important class of ASD-causing or related genes, and, among these synapse-associated ASD candidates, human genetic evidence has shown that cortactin-binding protein 2 (CTTNBP2) is a strong ASD candidate gene [22,23,24,25]. Gene modification studies at the cellular level and in animal models have linked ASD-associated mutations in CTTNBP2 to altered neuronal function and the production of autism-like behaviors [26]. The excitatory synapses in the mammalian nervous system are located in dendritic spines, which are the neuron-specific subcellular structures necessary for neural circuitry and function [27,28]. Recent evidence suggests that an alteration in dendritic spines may be involved in the pathogenesis of ASD [29,30].
Despite the complex genetic background of ASD, genetic research has largely advanced our understanding of the causes of ASD and identified the molecular pathways involved in the pathology. Whole-genome/exome sequencing, candidate gene resequencing, and copy number variation analysis have identified many ASD-related variants [31]. Currently, ASD can be described as a genetic model of a “common disease, many rare mutations” [32]. In the context of ASD-associated mutations exhibiting rare or even "private" but high penetrance; the genetic model also highlights the importance of identifying the functional pathways associated with ASD risk genes. This situation, on the one hand, may facilitate the discovery of new candidate genes for ASD, while, on the other hand, it may serve as a target for therapeutic drug intervention. Genetic studies targeting ASD have identified a large number of autism risk genes. To date, the SFARI Gene database includes more than 1000 ASD candidate genes. Among these genes, a subset of genes that are key regulators of synaptic plasticity has attracted increasing attention. These genes include those encoding synaptic proteins, small GTPases, cell adhesion molecules, and actin cytoskeletal proteins. The functional variants of these genes, identified in ASD families, have some clinical significance. This is mainly because the functional mutations in these genes may alter the synaptic strength or number, ultimately altering neuronal connectivity in the brain and increasing an individual’s risk of developing ASD. At the same time, they also hold promise for the development of targeted pharmacological intervention programs [15].

2. Materials and Methods

2.1. Participants

The proband was a 5-year-old boy who had been diagnosed with ASD in accordance with the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM-5), at 1 year and 6 months old. He was delivered at term by Cesarean section when his mother was 31 years old. There were no abnormalities at birth and no clear family history of neurological disorders. The proband could only say monosyllabic words, could not construct sentences, communicated little, often ignored people, only glanced at others when dealing with someone, did not actively participate in activities with other children, and liked rotating objects and mobile phones, as well as closing doors and switching things on and off. His Autistic Behavior Checklist score was 24, and his Childhood Autism Rating Scale score was 33. Brain magnetic resonance imaging examination showed no abnormal parenchymal brain. Emission computed tomography (ECT) examination showed focal perfusion and dysfunction (Supplementary Figure S1). We obtained informed consent from the parents of the child. This study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of the Children’s Hospital of Fudan University. Three to five milliliters of whole blood was drawn from all four members of the quad family. The Magbind Blood DNA Kit (CW Biotech, Beijing, China) was used to exact the genomic DNA in accordance with the manufacturer’s instructions, and the exacted DNA was stored in an ultra-low temperature refrigerator at −80 °C before use.

2.2. Chromosome Microarray

Chromosome microarrays were performed as described in previous reports [33,34]. Briefly, first, the Affymetrix CytoScanHD platform (Thermo Fisher, Hudson, NH, USA) was used for genotyping and completed according to the manufacturer’s instructions. Then, the ChAS software package (Thermo Fisher, Hudson, NH, USA) was used to identify copy number variants (CNVs); the cutoffs used are 200 kb for gain, 100 kb for loss, and 10,000 kb for the region of homozygosity (ROH). Then, the determined CNVs were annotated in accordance with the Database of Genomic Variants (DGV; http://projects.tcag.ca/variation/ (accessed on 26 November 2022)), after which the UCSC Browser based on GRCH37 was used to determine the gene composition of CNVs of interest. Finally, each of the CNVs was further evaluated using an in-house benign CNV database. At the same time, we analyzed the possibility that the affected genes were associated with the phenotypic characteristics of ASD observed in the patients.

2.3. Whole-Exome Sequencing

Based on the previously published study in [34], we completed whole-exome sequencing of the quad family. In brief, a Qubit dsDNA BR assay kit (Thermo Fisher, Hudson, NH, USA) was used to determine the DNA concentrations; then, the TruSeq DNA Sample Preparation kit (Illumina, San Diego, CA, USA) was used to construct 200–500-bp libraries, and 0.5 micrograms genomic DNA was used. Subsequently, SureSelect Human All-Exon V5 (Agilent, Santa Clara, CA, USA) was used to capture the targeted region in the libraries. Finally, the captured libraries were enriched, and Illumina Hiseq X-ten (Illumina, San Diego, CA, USA) was used to sequence the captured libraries.

2.4. Bioinformatic Analysis

The de novo and inherited variants of the proband were called a pipeline per our previous studies [11]. In brief, the reads with adaptor sequences or with low quality were first filtered out using Trimmomatic [35]. Subsequently, the Burrows–Wheeler Aligner [36] was used to align the remaining reads to the reference human genome (hg19), after which Picard (http://broadinstitute.github.io/picard/ (accessed on 25 August 2019)) was used to remove polymerase chain reaction (PCR) duplicates; then, Genome Analysis Toolkit [37] version 3.0 was used to call variants in each sample, followed by the use of mirTrios [38] to identify the de novo or rare inherited variants in the proband. Finally, ANNOVAR [39] was used to annotate the variants. To prioritize variants, we first filtered out the common variants (with a minor allele frequency of >0.05 in the 1000 Genomes Project database, gnomAD database, or our in-house database); then, we focused on the variants potentially associated with ASD according to the Simons Foundation Autism Research Initiative (SFARI) gene database [40] or predicted by Phenolyzer [41]. Finally, we focused on variants that might damage protein function as predicted by SIFT [42], PolyPhen-2 [43], and MutationTaster [44].

2.5. Sanger Sequencing

To confirm the candidate variant CTTNBP2, NM_033427, exon 3, c.344T > C, p.M115T, the ABI9700 PCR system (Thermo Fisher, Hudson, NH, USA) was used to perform a PCR using primers (forward, 5′-GTTGTGGGGAAAATGACCAT-3′; reverse, 5′-ATGACCCGTTCCTGGCACTC-3′). The ABI 3730xl sequencer (Thermo Fisher, Hudson, NH, USA) was used to sequence the obtained PCR product.

2.6. Genotyping

A total of 867 individuals not affected by ASD were recruited for validation of the variant frequency. The Magbind Blood DNA Kit (CW Biotech, Beijing, China) was used to extract the genomic DNA. CTTNBP2 p.M115T (c.344T > C) was further confirmed using the MassARRAY iPLEX platform (Agena Bioscience, San Diego, CA, USA), in accordance with the manufacturer’s manual [45].

2.7. Tertiary Structure Prediction

We used alphafold2 [46,47] to predict the tertiary structure of wild-type and mutant gene proteins based on their amino acid sequences. The maximum template date is 14 May 2022, and the AlphaFold model is a monomer.

2.8. Genetic Model Prediction

The prediction of the genetic model was done by DOMINO software [48]. The DOMINO score obtained was taken between 0 and 1, suggesting the probability of conforming to the dominant genetic model. The closer the score is to 0, the more likely it is the recessive genetic model; the closer the score is to 1, the more likely it is a dominant genetic model.

2.9. Genes Enrichment Analysis

The common variants, with an allele frequency greater than 0.05 in public databases, and synonymous mutations were filtered out first. Then, all variant genes were used to perform gene enrichment analysis. Finally, an enrichment analysis of all variant genes was performed by Metascape software [49], and the terms enriched included gene ontology (GO), pathway terms from the Kyoto Encyclopedia of Genes and Genomes (KEGG), canonical pathways, hallmark gene sets, etc.

3. Results

Variants Identified of the Proband

In order to find the genetic mutations associated with autism, we first examined the genome-wide copy number variation (CNV) by chromosome microarray (CMA) technology. The proband affected with ASD was first screened by CMA, which resulted in no clinically relevant CNVs being identified (Supplementary Figure S2). Then, the proband, together with his sister and parents who were not affected by ASD, were subjected to WES (Supplementary Table S1). WES yielded 12.12 Gbp, or 151-fold depth per sample; 98.65% of the targeted whole-exome region was covered at >20-fold depth, and the overall coverage rate was 99.89% per sample (Supplementary Table S2). On average, 22,218 exonic single-nucleotide variants (SNVs) (Supplementary Table S3) and 690 exonic small insertions and deletions (indels) (Supplementary Table S4) were called for each sample.
After initially filtering the public database (1000 genome and genome) for common variants and synonymous variants, a total of 541 variants were obtained (see Supplementary Table S5) from 248 genes in order to better perform gene enrichment analysis of non-SFARI genes and discover some biological pathways associated with the candidate autism genes. After removing the SFARI genes (Supplementary Table S6, total 1095 genes), a total of 218 non-SFARI genes were obtained. We performed a pathway enrichment analysis for both 248 genes and 218 non-SFARI genes using Metascape; the results are shown in Supplementary Figure S3. It can be seen that all 248 genes were enriched to delayed speech and language development and Byzantine-arch palate pathways; the 218 non-SFARI genes were enriched to hyperinsulinism and Byzantine-arch palate pathways.
Considering that we have whole-exome sequencing data for the complete quad family, we can easily distinguish the variants found in the proband into inherited or de novo variants, and all of the variants in the proband were used together to find the clinically relevant variants in autism. Through systematic bioinformatic analysis (Figure 1), we identified candidate variants in some known ASD-related genes [40]. Combining this with an analysis using a phenotype-based prioritization tool [41], p.M115T (chr7: 117450889, CTTNBP2, NM_033427, exon 3, c.344T > C), a de novo missense variant in CTTNBP2, was shown to be associated with ASD (Figure 2a, Supplementary Table S7). This variant was further confirmed by Sanger sequencing (Figure 2b).
To determine the allele frequency of nonsynonymous mutations of the CTTNBP2 gene in the general population, we downloaded the CTTNBP2 gene variants included in the gnomAD database (see Supplementary Table S8) and performed the statistics. We found that the allele frequency of missense mutations in the CTTNBP2 gene ranged from 0 to 0.0019 in the East Asian population, which is relatively rare; notably, we found that the allele frequency of missense mutations in the CTTNBP2 gene located in the coiled-coil (CC) region ranged from 0.000283 to 0.00000397, which is very rare. Notably, we found the presence of variant p.Met115Lys in the CTTNBP2 gene, which is rare in the population (only 1 detected in 31,392 individuals) and rare in the East Asian population (0 detected in 1560 East Asians).
Considering that the collection of a Chinese population may be limited to the East Asian population of the gnomAD database, we performed further large-scale validation of M115T in the Chinese population. The MassARRAY confirmed that the variant was rare (0 in 867) in Chinese individuals not affected by ASD (Supplementary Table S9).
The CTTNBP2 (cortactin-binding protein 2) gene belongs to a family of ankyrin repeat domain-containing proteins that function in protein–protein interactions; the missense variant p.M115T leads to a change from methionine to threonine at amino acid sequence position 115; the missense variant p.M115T was predicted by SIFT [42], PolyPhen2 [43], and MutationTaster [44] to damage the function of the CTTNBP2 protein. The variant p.M115T is located in the N-terminal domain of CTTNBP2. CTTNBP2 was also identified as an ASD-associated gene and classified as having “suggestive evidence” of this by the SFARI Gene database [40].

4. Discussion

ASD affects around 1% of children with no effective blood test or cure [50]. Studies have shown that genetic mechanisms play an important role in the pathogenesis of ASD [51,52]. To date, over 1000 autism-related genes have been included in the SFARI database. These genes help to understand the etiology of autism occurrence and identify potential pharmacological interventions. Genetic mutations of these genes found in autism-affected families are an important reference for the diagnosis of autism. Currently, chromosome microarray, together with high-throughput sequencing has been used for clinical genetic diagnosis of ASD [53]. We have accumulated some experience in discovering autism-associated variants by high-throughput sequencing of trio or quad families affected by autism. With 32 ASD Canadian families with negative CMA results, we identified clinically relevant variants in 16 of 32 families and developed a pipeline to identify rare de novo and inherited variants relevant to ASD [11]. In the present study, a quad family affected by ASD was recruited. The proband was first screened by CMA with negative results, after which WES was performed on the proband, together with his sister and his parents who were not affected by ASD. Through systematic bioinformatic analysis, a de novo mutation of CTTNBP2, p.M115T, was successfully identified and confirmed by Sanger sequencing as the clinically relevant variant in the proband. This shows that family-based WES is a viable method for genetic testing for ASD.
The CTTNBP2 gene is located on chromosome 7q31, a candidate region for autism [54]. The CTTNBP2 gene encodes a neuron-specific cytoskeleton protein, which controls the formation and maintenance of dendritic spines by interacting with cortactin [55,56]. Cortactin is a ubiquitous protein that regulates the branching and stability of F-actin. Although cortactin is not neuron-specific, it is widely distributed in the dendritic spine area and is thought to regulate the remodeling of neuronal activity. Based on the analysis of the secondary structure of an amino acid sequence, the CTTNBP2 protein is composed of an N-terminal coiled-coil domain and a C-terminal intrinsically disordered region (IDR) [26]. The coiled-coil domain mediates the oligomerization of CTTNBP2 as well as both homopolymerization and heteropolymerization with the striatum [56]. The IDR region at the C-terminal consists of two regions, namely an intermediate domain responsible for microtubule interaction and a proline-rich region and a region of about 100 amino acids that mediates the interaction with cortactin (Figure 3a, refer to [26]). The M115T mutation found in the coiled-coil domain is noteworthy because Shih et al. found that the M120I mutation is also located in the coiled-coil nearby and has a negative impact on the interaction between CTTNBP2 and cortactin. After introducing the patient’s CTTNBP2 gene with the heterozygous mutation M120I into mice, the mice exhibited reduced social interactions [26]. Considering that M115T and M120I are more proximate (Figure 3b), it is not difficult to speculate that M115T may also play a very important role in ASD.
So far, 38 nonsynonymous mutations in the CTTNBP2 gene have been identified in ASD patients (Supplementary Table S10), and most of these mutations are located in the proline-rich region and the C-terminus (Figure 3c). It is known that the C-terminus mediates the binding of the CTTNBP2 protein to the cortactin protein and may have a more direct effect on CTTNBP2 function than other regions. Shih et al. found that mutations in R533*, located in the proline-rich region, directly led to a reduction in the number of synaptic spines [26,57]. A recent study revealed that zinc binds to the N-terminal coiled-coil region of CTTNBP2 and promotes higher-order assembly, which can rescue the autistic phenotype in mice [58]. The CTTNBP2 gene is a known strong autism candidate gene in the SFARI database. We identified a rare de novo missense mutation, p.M115T, in the CTTNBP2 gene in patients affected by autism. This mutation is of interest because of its location in the N-terminal coiled-coil (CC) region, which mediates the oligomerization of the CTTNBP2 protein and thus regulates spine formation. The next step, which is in-depth functional validation, is required to confirm whether this variant directly affects spine formation and the causal relationship with autism.
Considering the complexity of the genetic model of autism, the currently identified autism genes sometimes conform to an autosomal recessive genetic model, sometimes to an autosomal dominant genetic model, and sometimes are difficult to explain by the conventional genetic model. No other mutations have been found in the CTTNBP2 gene, except for the de novo heterozygous mutation in p.M115T. Moreover, studies in animal models have shown that the introduction of heterozygous mutations into the CTTNBP2 gene can lead to behavioral defects in mice. These suggest that the probability of being consistent with autosomal dominant is greater than autosomal recessive. The genetic model of autism is complex, with the emerging double-hit, or multiple-hit hypotheses [59], that is, mutations in two or more genes together cause autism. In particular, this hypothesis has also been used by some scholars when interpreting the detection of de novo mutations in autistic patients [60]. Finally, based on the results of DOMINO, the possibility that p.M115T de novo heterozygous mutations is consistent with the double-hit or multiple-hit hypotheses cannot be ruled out either. Also there is the possibility that the variant could be functioning molecularly as recessive while functioning genetically as dominant. For example, if the detected variant is causing excessive polymerization, of CTTNBP2, there may be little oligomeric or monomeric CTTNBP2 available to interact with other cellular interactors, resulting in insufficient CTTNBP2 functional activity. This is molecularly recessive, consistent with the results of the DOMINO analysis, but also would be genetically dominant.
Highly specialized neuronal morphology provides a substrate for constructing human cognitive and behavioral neural circuits, and neuron morphogenesis is a very complex process, which is affected by both genetic susceptibility and environmental events [61]. In the mammalian nervous system, dendritic spines are located at excitatory synaptic sites; they are neuron-specific subcellular structures that are necessary for neural circuitry and function. Hundreds of genes are known to be involved in the regulation of dendritic spine formation; most of these genes are non-neuron specific, including ANXA1, syndecan-2, NF1 (encoding neurofibromin), VCP (valosin-containing protein), CASK, and CTTNBP2 [29,62]. CTTNBP2 is a neuron-specific gene that regulates dendritic spine formation [29]. CTTNBP2 is located in a region associated with ASD, namely, the autism candidate region at 7q31 [54]. Iossifov et al. first identified a de novo 2-bp frameshift deletion at this site in patients affected with ASD [23], which suggested that CTTNBP2 is an ASD-associated gene. In agreement with this, we found a de novo mutation of CTTNBP2, p.M115T. This mutation, located at the N-terminus-binding domain, leads to a change from methionine to threonine at amino acid position 115 and may affect protein–protein interactions. CTTNBP2 plays an important role in regulating dendritic spine morphology and synaptic plasticity [55] in synapse formation. Furthermore, MacGillavry et al. found that cortactin can interact with Shank to control actin dynamics and maintain the flexibility of neuronal spines and synapses. A mutation of CTTNBP2 that might damage the function of the protein it encodes could thus potentially lead to abnormal brain connectivity [63]. In agreement with this, an ECT image of the proband showed abnormal focal perfusion. The present study suggests that family WES is a viable approach for identifying the genetic mutations associated with ASD. The CTTNBP2 mutation that we found in this study may well explain the patient’s phenotype.

5. Conclusions

We successfully identified a clinically relevant de novo mutation in the CTTNBP2 gene in a quad family affected by ASD. Considering the neuron-specific expression of CTTNBP2 and its role in neuronal and dendritic spine formation, this finding provides genetic evidence for the spine pathology of ASD. However, this study has some limitations: First, we do not know whether this de novo mutation of the CTTNBP2 gene is common in ASD patients or how this mutation participates in the occurrence of ASD, so screening for the CTTNBP2 mutation in large-scale ASD patients and in-depth functional studies will be necessary to determine the role of CTTNBP2 in the pathogenesis of ASD. Moreover, common genetic variants do play an important role in autism, and, considering the limited sample of this study, we did not focus on common genetic variants. In conclusion, although the present study is currently insufficient to confirm that the de novo mutation M115T in CTTNBP2 directly causes the autism phenotype, our study provides support for the assertion that CTTNBP2 is a strong candidate gene in autism.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/children10010080/s1, Figure S1: Emission Computed Tomography showed focal perfusion and dysfunction; Figure S2: Chromosome Microarray Report of proband; Figure S3: Genes Enriched analysis; Table S1: Quad family description; Table S2: Whole Exome Sequencing performance; Table S3: SNP statistics; Table S4: Indel statistics; Table S5: 541 rare nonsynonymous variantsvariants identified in Proband; Table S6: 1095 SFARI genes; Table S7: de novo missense variant in CTTNBP2 identified in the proband; Table S8: CTTNBP2 varints in gnomAD database; Table S9: Genotyping results of CTTNBP2 c.344T > C in additional 867 non-ASDs individuals; Table S10: 38 nonsynonymous mutations in the CTTNBP2 gene have been identified in ASD patients.

Author Contributions

Y.X., H.W., B.H. and M.W. wrote the first draft of the manuscript. X.Z. performed the statistical analysis. A.L. and C.C. (Chunquan Cai) performed the assessment of autism. S.L. performed the assessment of autism and the imaging evaluation. C.C. (Cheng Chen), Z.W. and Z.Y. performed the clinical analysis. M.W. contributed to the conception and design of this study and guided the manuscript. All authors have read and agreed to the published version of the manuscript.

Funding

This project was supported by the National Natural Science Foundation of China (Program Nos. 82071733, 81960290, and 81971603), the Shenzhen Science Technology and Innovation Commission (JCYJ20220530154601004), the Public Health and Technology Project of Tianjin (No. TJWJ2021ZD007), the Program of Tianjin Science and Technology Plan (No. 21JCZDJC00390), Shanghai Talent Development Funding (No. 2020115), the Beijing Municipal Science & Technology Commission (Z181100001518005), the Beijing Municipal Commission of Health and Family Planning (CFH2018-2Z-5012), and the High-Level Project of Medicine of Longhua (No. HLPM201907020103).

Institutional Review Board Statement

This study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Committee of the Children’s Hospital of Fudan University (Approval Code: No. 2020-56, Approval Date: 1 January 2021).

Informed Consent Statement

Written informed consent has been obtained from the patient’s parents to publish this paper.

Data Availability Statement

The data used in this study have been uploaded to the supplementary annex. Considering the control of human genetic resources, the original data have not been uploaded to the public database, and the VCF file for research can be obtained from the corresponding author.

Acknowledgments

We thank the children and parents who participated in the present study.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Lord, C.; Charman, T.; Havdahl, A.; Carbone, P.; Anagnostou, E.; Boyd, B.; Carr, T.; de Vries, P.J.; Dissanayake, C.; Divan, G.; et al. The Lancet Commission on the future of care and clinical research in autism. Lancet 2022, 399, 271–334. [Google Scholar] [CrossRef] [PubMed]
  2. Li, X.; Zhang, K.; He, X.; Zhou, J.; Jin, C.; Shen, L.; Gao, Y.; Tian, M.; Zhang, H. Structural, Functional, and Molecular Imaging of Autism Spectrum Disorder. Neurosci. Bull. 2021, 37, 1051–1071. [Google Scholar] [CrossRef] [PubMed]
  3. Colvert, E.; Tick, B.; McEwen, F.; Stewart, C.; Curran, S.R.; Woodhouse, E.; Gillan, N.; Hallett, V.; Lietz, S.; Garnett, T.; et al. Heritability of Autism Spectrum Disorder in a UK Population-Based Twin Sample. JAMA Psychiatry 2015, 72, 415–423. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Constantino, J.N.; Zhang, Y.; Frazier, T.; Abbacchi, A.M.; Law, P. Sibling recurrence and the genetic epidemiology of autism. Am. J. Psychiatry 2010, 167, 1349–1356. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Sandin, S.; Lichtenstein, P.; Kuja-Halkola, R.; Larsson, H.; Hultman, C.M.; Reichenberg, A. The familial risk of autism. JAMA 2014, 311, 1770–1777. [Google Scholar] [CrossRef]
  6. Bai, D.; Yip, B.H.K.; Windham, G.C.; Sourander, A.; Francis, R.; Yoffe, R.; Glasson, E.; Mahjani, B.; Suominen, A.; Leonard, H.; et al. Association of Genetic and Environmental Factors With Autism in a 5-Country Cohort. JAMA Psychiatry 2019, 76, 1035–1043. [Google Scholar] [CrossRef]
  7. Maenner, M.J.; Shaw, K.A.; Bakian, A.V.; Bilder, D.A.; Durkin, M.S.; Esler, A.; Furnier, S.M.; Hallas, L.; Hall-Lande, J.; Hudson, A.; et al. Prevalence and Characteristics of Autism Spectrum Disorder Among Children Aged 8 Years—Autism and Developmental Disabilities Monitoring Network, 11 Sites, United States, 2018. MMWR Surveill. Summ. 2021, 70, 1–16. [Google Scholar] [CrossRef]
  8. Yasuda, Y.; Matsumoto, J.; Miura, K.; Hasegawa, N.; Hashimoto, R. Genetics of autism spectrum disorders and future direction. J. Hum. Genet. 2022. [Google Scholar] [CrossRef]
  9. Wang, T.; Zhao, P.A.; Eichler, E.E. Rare variants and the oligogenic architecture of autism. Trends Genet. 2022, 38, 895–903. [Google Scholar] [CrossRef]
  10. Wang, M.; Fan, X.; Wang, T.; Wu, J. High-throughput sequencing of autism spectrum disorders comes of age. Genet. Res. 2013, 95, 121–129. [Google Scholar] [CrossRef]
  11. Jiang, Y.H.; Yuen, R.K.; Jin, X.; Wang, M.; Chen, N.; Wu, X.; Ju, J.; Mei, J.; Shi, Y.; He, M.; et al. Detection of clinically relevant genetic variants in autism spectrum disorder by whole-genome sequencing. Am. J. Hum. Genet. 2013, 93, 249–263. [Google Scholar] [CrossRef] [Green Version]
  12. Yuen, R.K.; Merico, D.; Cao, H.; Pellecchia, G.; Alipanahi, B.; Thiruvahindrapuram, B.; Tong, X.; Sun, Y.; Cao, D.; Zhang, T.; et al. Genome-wide characteristics of de novo mutations in autism. NPJ Genom. Med. 2016, 1, 16027. [Google Scholar] [CrossRef] [Green Version]
  13. Wu, J.; Yu, P.; Jin, X.; Xu, X.; Li, J.; Li, Z.; Wang, M.; Wang, T.; Wu, X.; Jiang, Y.; et al. Genomic landscapes of Chinese sporadic autism spectrum disorders revealed by whole-genome sequencing. J. Genet. Genom. 2018, 45, 527–538. [Google Scholar] [CrossRef]
  14. Talli, I.; Dovrolis, N.; Oulas, A.; Stavrakaki, S.; Makedou, K.; Spyrou, G.M.; Maroulakou, I. Novel clinical, molecular and bioinformatics insights into the genetic background of autism. Hum. Genom. 2022, 16, 39. [Google Scholar] [CrossRef]
  15. Willsey, H.R.; Willsey, A.J.; Wang, B.; State, M.W. Genomics, convergent neuroscience and progress in understanding autism spectrum disorder. Nat. Rev. Neurosci. 2022, 23, 323–341. [Google Scholar] [CrossRef]
  16. Manoli, D.S.; State, M.W. Autism Spectrum Disorder Genetics and the Search for Pathological Mechanisms. Am. J. Psychiatry 2021, 178, 30–38. [Google Scholar] [CrossRef]
  17. Paulsen, B.; Velasco, S.; Kedaigle, A.J.; Pigoni, M.; Quadrato, G.; Deo, A.J.; Adiconis, X.; Uzquiano, A.; Sartore, R.; Yang, S.M.; et al. Autism genes converge on asynchronous development of shared neuron classes. Nature 2022, 602, 268–273. [Google Scholar] [CrossRef]
  18. Velmeshev, D.; Schirmer, L.; Jung, D.; Haeussler, M.; Perez, Y.; Mayer, S.; Bhaduri, A.; Goyal, N.; Rowitch, D.H.; Kriegstein, A.R. Single-cell genomics identifies cell type-specific molecular changes in autism. Science 2019, 364, 685–689. [Google Scholar] [CrossRef]
  19. Quesnel-Vallieres, M.; Weatheritt, R.J.; Cordes, S.P.; Blencowe, B.J. Autism spectrum disorder: Insights into convergent mechanisms from transcriptomics. Nat. Rev. Genet. 2019, 20, 51–63. [Google Scholar] [CrossRef]
  20. Adhya, D.; Swarup, V.; Nagy, R.; Dutan, L.; Shum, C.; Valencia-Alarcon, E.P.; Jozwik, K.M.; Mendez, M.A.; Horder, J.; Loth, E.; et al. Atypical Neurogenesis in Induced Pluripotent Stem Cells From Autistic Individuals. Biol. Psychiatry 2021, 89, 486–496. [Google Scholar] [CrossRef]
  21. Sacai, H.; Sakoori, K.; Konno, K.; Nagahama, K.; Suzuki, H.; Watanabe, T.; Watanabe, M.; Uesaka, N.; Kano, M. Autism spectrum disorder-like behavior caused by reduced excitatory synaptic transmission in pyramidal neurons of mouse prefrontal cortex. Nat. Commun. 2020, 11, 5140. [Google Scholar] [CrossRef] [PubMed]
  22. De Rubeis, S.; He, X.; Goldberg, A.P.; Poultney, C.S.; Samocha, K.; Cicek, A.E.; Kou, Y.; Liu, L.; Fromer, M.; Walker, S.; et al. Synaptic, transcriptional and chromatin genes disrupted in autism. Nature 2014, 515, 209–215. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  23. Iossifov, I.; Ronemus, M.; Levy, D.; Wang, Z.; Hakker, I.; Rosenbaum, J.; Yamrom, B.; Lee, Y.H.; Narzisi, G.; Leotta, A.; et al. De novo gene disruptions in children on the autistic spectrum. Neuron 2012, 74, 285–299. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  24. Sanders, S.J.; He, X.; Willsey, A.J.; Ercan-Sencicek, A.G.; Samocha, K.E.; Cicek, A.E.; Murtha, M.T.; Bal, V.H.; Bishop, S.L.; Dong, S.; et al. Insights into Autism Spectrum Disorder Genomic Architecture and Biology from 71 Risk Loci. Neuron 2015, 87, 1215–1233. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Ruzzo, E.K.; Perez-Cano, L.; Jung, J.Y.; Wang, L.K.; Kashef-Haghighi, D.; Hartl, C.; Singh, C.; Xu, J.; Hoekstra, J.N.; Leventhal, O.; et al. Inherited and De Novo Genetic Risk for Autism Impacts Shared Networks. Cell 2019, 178, 850–866.e26. [Google Scholar] [CrossRef] [Green Version]
  26. Shih, P.Y.; Hsieh, B.Y.; Tsai, C.Y.; Lo, C.A.; Chen, B.E.; Hsueh, Y.P. Autism-linked mutations of CTTNBP2 reduce social interaction and impair dendritic spine formation via diverse mechanisms. Acta Neuropathol. Commun. 2020, 8, 185. [Google Scholar] [CrossRef]
  27. Chen, C.; Wei, J.; Ma, X.; Xia, B.; Shakir, N.; Zhang, J.K.; Zhang, L.; Cui, Y.; Ferguson, D.; Qiu, S.; et al. Disrupted Maturation of Prefrontal Layer 5 Neuronal Circuits in an Alzheimer’s Mouse Model of Amyloid Deposition. Neurosci. Bull. 2022. [Google Scholar] [CrossRef]
  28. Song, X.L.; Liu, D.S.; Qiang, M.; Li, Q.; Liu, M.G.; Li, W.G.; Qi, X.; Xu, N.J.; Yang, G.; Zhu, M.X.; et al. Postsynaptic Targeting and Mobility of Membrane Surface-Localized hASIC1a. Neurosci. Bull. 2021, 37, 145–165. [Google Scholar] [CrossRef]
  29. Hu, H.T.; Shih, P.Y.; Shih, Y.T.; Hsueh, Y.P. The Involvement of Neuron-Specific Factors in Dendritic Spinogenesis: Molecular Regulation and Association with Neurological Disorders. Neural Plast. 2016, 2016, 5136286. [Google Scholar] [CrossRef] [Green Version]
  30. Nanou, E.; Catterall, W.A. Calcium Channels, Synaptic Plasticity, and Neuropsychiatric Disease. Neuron 2018, 98, 466–481. [Google Scholar] [CrossRef]
  31. Vorstman, J.A.S.; Parr, J.R.; Moreno-De-Luca, D.; Anney, R.J.L.; Nurnberger, J.I., Jr.; Hallmayer, J.F. Autism genetics: Opportunities and challenges for clinical translation. Nat. Rev. Genet. 2017, 18, 362–376. [Google Scholar] [CrossRef]
  32. McClellan, J.; King, M.C. Genetic heterogeneity in human disease. Cell 2010, 141, 210–217. [Google Scholar] [CrossRef] [Green Version]
  33. Xie, Y.; Yuan, H.; Wang, M.; Zhong, L.; Zhou, J.; Song, B.; Yin, Q.; Sun, X. Copy number variations independently induce autism spectrum disorder. Biosci. Rep. 2017, 37, BSR20160570. [Google Scholar]
  34. Zhou, J.; Yang, F.; Li, X.; Zhou, S.; He, F.; Liang, J.; Wang, Y.; Wang, M.; Xu, R. Whole Exome Sequencing Revealed Clinical Relevant Variants in A Family Affected with Autism Spectrum Disorder. J. Psychiatry Brain Sci. 2016, 1, 3. [Google Scholar]
  35. Bolger, A.M.; Lohse, M.; Usadel, B. Trimmomatic: A flexible trimmer for Illumina sequence data. Bioinformatics 2014, 30, 2114–2120. [Google Scholar] [CrossRef] [Green Version]
  36. Li, H.; Durbin, R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 2009, 25, 1754–1760. [Google Scholar] [CrossRef] [Green Version]
  37. McKenna, A.; Hanna, M.; Banks, E.; Sivachenko, A.; Cibulskis, K.; Kernytsky, A.; Garimella, K.; Altshuler, D.; Gabriel, S.; Daly, M.; et al. The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 2010, 20, 1297–1303. [Google Scholar] [CrossRef] [Green Version]
  38. Li, J.; Jiang, Y.; Wang, T.; Chen, H.; Xie, Q.; Shao, Q.; Ran, X.; Xia, K.; Sun, Z.S.; Wu, J. mirTrios: An integrated pipeline for detection of de novo and rare inherited mutations from trios-based next-generation sequencing. J. Med. Genet. 2015, 52, 275–281. [Google Scholar] [CrossRef]
  39. Wang, K.; Li, M.; Hakonarson, H. ANNOVAR: Functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Res. 2010, 38, e164. [Google Scholar] [CrossRef]
  40. Abrahams, B.S.; Arking, D.E.; Campbell, D.B.; Mefford, H.C.; Morrow, E.M.; Weiss, L.A.; Menashe, I.; Wadkins, T.; Banerjee-Basu, S.; Packer, A. SFARI Gene 2.0: A community-driven knowledgebase for the autism spectrum disorders (ASDs). Mol. Autism 2013, 4, 36. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  41. Yang, H.; Robinson, P.N.; Wang, K. Phenolyzer: Phenotype-based prioritization of candidate genes for human diseases. Nat. Methods 2015, 12, 841–843. [Google Scholar] [CrossRef] [PubMed]
  42. Kumar, P.; Henikoff, S.; Ng, P.C. Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm. Nat. Protoc. 2009, 4, 1073–1081. [Google Scholar] [CrossRef] [PubMed]
  43. Adzhubei, I.A.; Schmidt, S.; Peshkin, L.; Ramensky, V.E.; Gerasimova, A.; Bork, P.; Kondrashov, A.S.; Sunyaev, S.R. A method and server for predicting damaging missense mutations. Nat. Methods 2010, 7, 248–249. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Schwarz, J.M.; Rodelsperger, C.; Schuelke, M.; Seelow, D. MutationTaster evaluates disease-causing potential of sequence alterations. Nat. Methods 2010, 7, 575–576. [Google Scholar] [CrossRef] [PubMed]
  45. Gabriel, S.; Ziaugra, L.; Tabbaa, D. SNP genotyping using the Sequenom MassARRAY iPLEX platform. Curr. Protoc. Hum. Genet. 2009, 60, 2.12.1–2.12.18. [Google Scholar] [CrossRef]
  46. Jumper, J.; Evans, R.; Pritzel, A.; Green, T.; Figurnov, M.; Ronneberger, O.; Tunyasuvunakool, K.; Bates, R.; Zidek, A.; Potapenko, A.; et al. Highly accurate protein structure prediction with AlphaFold. Nature 2021, 596, 583–589. [Google Scholar] [CrossRef]
  47. Varadi, M.; Anyango, S.; Deshpande, M.; Nair, S.; Natassia, C.; Yordanova, G.; Yuan, D.; Stroe, O.; Wood, G.; Laydon, A.; et al. AlphaFold Protein Structure Database: Massively expanding the structural coverage of protein-sequence space with high-accuracy models. Nucleic Acids Res. 2022, 50, D439–D444. [Google Scholar] [CrossRef]
  48. Quinodoz, M.; Royer-Bertrand, B.; Cisarova, K.; Di Gioia, S.A.; Superti-Furga, A.; Rivolta, C. DOMINO: Using Machine Learning to Predict Genes Associated with Dominant Disorders. Am. J. Hum. Genet. 2017, 101, 623–629. [Google Scholar] [CrossRef] [Green Version]
  49. Zhou, Y.; Zhou, B.; Pache, L.; Chang, M.; Khodabakhshi, A.H.; Tanaseichuk, O.; Benner, C.; Chanda, S.K. Metascape provides a biologist-oriented resource for the analysis of systems-level datasets. Nat. Commun. 2019, 10, 1523. [Google Scholar] [CrossRef]
  50. Jeste, S.S.; Geschwind, D.H. Disentangling the heterogeneity of autism spectrum disorder through genetic findings. Nat. Rev. Neurol. 2014, 10, 74–81. [Google Scholar] [CrossRef] [Green Version]
  51. Chen, J.A.; Penagarikano, O.; Belgard, T.G.; Swarup, V.; Geschwind, D.H. The emerging picture of autism spectrum disorder: Genetics and pathology. Annu. Rev. Pathol. 2015, 10, 111–144. [Google Scholar] [CrossRef]
  52. de la Torre-Ubieta, L.; Won, H.; Stein, J.L.; Geschwind, D.H. Advancing the understanding of autism disease mechanisms through genetics. Nat. Med. 2016, 22, 345–361. [Google Scholar] [CrossRef]
  53. Tammimies, K.; Marshall, C.R.; Walker, S.; Kaur, G.; Thiruvahindrapuram, B.; Lionel, A.C.; Yuen, R.K.; Uddin, M.; Roberts, W.; Weksberg, R.; et al. Molecular Diagnostic Yield of Chromosomal Microarray Analysis and Whole-Exome Sequencing in Children With Autism Spectrum Disorder. JAMA 2015, 314, 895–903. [Google Scholar] [CrossRef]
  54. Cheung, J.; Petek, E.; Nakabayashi, K.; Tsui, L.C.; Vincent, J.B.; Scherer, S.W. Identification of the human cortactin-binding protein-2 gene from the autism candidate region at 7q31. Genomics 2001, 78, 7–11. [Google Scholar] [CrossRef]
  55. Chen, Y.K.; Hsueh, Y.P. Cortactin-binding protein 2 modulates the mobility of cortactin and regulates dendritic spine formation and maintenance. J. Neurosci. Off. J. Soc. Neurosci. 2012, 32, 1043–1055. [Google Scholar] [CrossRef] [Green Version]
  56. Chen, Y.K.; Chen, C.Y.; Hu, H.T.; Hsueh, Y.P. CTTNBP2, but not CTTNBP2NL, regulates dendritic spinogenesis and synaptic distribution of the striatin-PP2A complex. Mol. Biol. Cell 2012, 23, 4383–4392. [Google Scholar] [CrossRef]
  57. Shih, P.Y.; Hsieh, B.Y.; Lin, M.H.; Huang, T.N.; Tsai, C.Y.; Pong, W.L.; Lee, S.P.; Hsueh, Y.P. CTTNBP2 Controls Synaptic Expression of Zinc-Related Autism-Associated Proteins and Regulates Synapse Formation and Autism-like Behaviors. Cell Rep. 2020, 31, 107700. [Google Scholar] [CrossRef]
  58. Shih, P.Y.; Fang, Y.L.; Shankar, S.; Lee, S.P.; Hu, H.T.; Chen, H.; Wang, T.F.; Hsia, K.C.; Hsueh, Y.P. Phase separation and zinc-induced transition modulate synaptic distribution and association of autism-linked CTTNBP2 and SHANK3. Nat. Commun. 2022, 13, 2664. [Google Scholar] [CrossRef]
  59. Saldarriaga, W.; Payan-Gomez, C.; Gonzalez-Teshima, L.Y.; Rosa, L.; Tassone, F.; Hagerman, R.J. Double Genetic Hit: Fragile X Syndrome and Partial Deletion of Protein Patched Homolog 1 Antisense as Cause of Severe Autism Spectrum Disorder. J. Dev. Behav. Pediatr. 2020, 41, 724–728. [Google Scholar] [CrossRef]
  60. Guo, H.; Wang, T.; Wu, H.; Long, M.; Coe, B.P.; Li, H.; Xun, G.; Ou, J.; Chen, B.; Duan, G.; et al. Inherited and multiple de novo mutations in autism/developmental delay risk genes suggest a multifactorial model. Mol. Autism 2018, 9, 64. [Google Scholar] [CrossRef]
  61. Tau, G.Z.; Peterson, B.S. Normal development of brain circuits. Neuropsychopharmacology 2010, 35, 147–168. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  62. Zheng, L.; Wang, Y.; Shao, B.; Zhou, H.; Li, X.; Zhang, C.; Sun, N.; Shi, J. Multiple Mild Stimulations Reduce Membrane Distribution of CX3CR1 Promoted by Annexin a1 in Microglia to Attenuate Excessive Dendritic Spine Pruning and Cognitive Deficits Caused by a Transient Ischemic Attack in Mice. Neurosci. Bull. 2022, 38, 753–768. [Google Scholar] [CrossRef] [PubMed]
  63. MacGillavry, H.D.; Kerr, J.M.; Kassner, J.; Frost, N.A.; Blanpied, T.A. Shank-cortactin interactions control actin dynamics to maintain flexibility of neuronal spines and synapses. Eur. J. Neurosci. 2016, 43, 179–193. [Google Scholar] [CrossRef] [PubMed]
Children 10 00080 g001 550
Figure 1. Flowchart for detecting clinically relevant variants in ASD by CMA and WES. ASD, autism spectrum disorder; CMA, chromosome microarray; HPO, human phenotype ontology; MAF, minor allele frequency; SFARI, Simons Foundation Autism Research Initiative; VOUS, variant of uncertain clinical significance; WES, whole-exome sequencing. First, we filtered for common variants with an allele frequency greater than 0.05; then, we evaluated the pathogenicity of the variants with reference to the ACMG guidelines, together with the SFARI gene score to identify candidate clinically relevant variants for autism. Meanwhile, considering the limited data on the Chinese population within the East Asian data in gnomAD databases, we validated clinically relevant variants that could be candidates for autism in 867 normal populations.
Figure 1. Flowchart for detecting clinically relevant variants in ASD by CMA and WES. ASD, autism spectrum disorder; CMA, chromosome microarray; HPO, human phenotype ontology; MAF, minor allele frequency; SFARI, Simons Foundation Autism Research Initiative; VOUS, variant of uncertain clinical significance; WES, whole-exome sequencing. First, we filtered for common variants with an allele frequency greater than 0.05; then, we evaluated the pathogenicity of the variants with reference to the ACMG guidelines, together with the SFARI gene score to identify candidate clinically relevant variants for autism. Meanwhile, considering the limited data on the Chinese population within the East Asian data in gnomAD databases, we validated clinically relevant variants that could be candidates for autism in 867 normal populations.
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Figure 2. De novo CTTNBP2 variant confirmed in a quad family affected by ASD: (a) CTTNBP2 p.M115T (c.344T > C) was identified in the proband as a de novo mutation (II-1), +, wild type; (b) Sanger sequencing confirmed the presence of CTTNBP2 p.M115T (c.344T > C) in the proband as a de novo mutation.
Figure 2. De novo CTTNBP2 variant confirmed in a quad family affected by ASD: (a) CTTNBP2 p.M115T (c.344T > C) was identified in the proband as a de novo mutation (II-1), +, wild type; (b) Sanger sequencing confirmed the presence of CTTNBP2 p.M115T (c.344T > C) in the proband as a de novo mutation.
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Figure 3. Structure of the M115T mutation in the CTTNBP2 encoded protein and known CTTNBP2 mutation statistics. (a) Illustration of the important structural domain of the protein encoded by the CTTNBP2 gene. The M115T mutation is located in the N-terminal coiled-coil (CC) region, which mediates the oligomerization of the CTTNBP2 protein and can affect the CTTNBP2 protein binding to the cortactin protein. Shih et al. found that the M120I mutation in the N-terminal region of CTTNBP2 protein negatively affected the interaction of CTTNBP2 with cortactin; mice exhibited reduced social interaction after introducing the patient’s CTTNBP2 gene heterozygous mutation M120I into mice [26]. The M115T mutation identified in the present study is very close to M120I. (b) A 3D structural illustration of the protein encoding CTTNBP2, obtained by AlphaFold [46,47] Protein Structure Database (https://alphafold.com/entry/Q8WZ74, accessed on 1 November 2022). (c) Bar plot of ASD risk variants identified in the CTTNBP2 gene according to different regions featuring 39 variants in SFARI gene database (https://gene.sfari.org/database/human-gene/CTTNBP2#variants-tab (accessed on 1 November 2022)); one of the synonymous mutations was removed.
Figure 3. Structure of the M115T mutation in the CTTNBP2 encoded protein and known CTTNBP2 mutation statistics. (a) Illustration of the important structural domain of the protein encoded by the CTTNBP2 gene. The M115T mutation is located in the N-terminal coiled-coil (CC) region, which mediates the oligomerization of the CTTNBP2 protein and can affect the CTTNBP2 protein binding to the cortactin protein. Shih et al. found that the M120I mutation in the N-terminal region of CTTNBP2 protein negatively affected the interaction of CTTNBP2 with cortactin; mice exhibited reduced social interaction after introducing the patient’s CTTNBP2 gene heterozygous mutation M120I into mice [26]. The M115T mutation identified in the present study is very close to M120I. (b) A 3D structural illustration of the protein encoding CTTNBP2, obtained by AlphaFold [46,47] Protein Structure Database (https://alphafold.com/entry/Q8WZ74, accessed on 1 November 2022). (c) Bar plot of ASD risk variants identified in the CTTNBP2 gene according to different regions featuring 39 variants in SFARI gene database (https://gene.sfari.org/database/human-gene/CTTNBP2#variants-tab (accessed on 1 November 2022)); one of the synonymous mutations was removed.
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Xie, Y.; Wang, H.; Hu, B.; Zhang, X.; Liu, A.; Cai, C.; Li, S.; Chen, C.; Wang, Z.; Yin, Z.; et al. Dendritic Spine in Autism Genetics: Whole-Exome Sequencing Identifying De Novo Variant of CTTNBP2 in a Quad Family Affected by Autism Spectrum Disorder. Children 2023, 10, 80. https://doi.org/10.3390/children10010080

AMA Style

Xie Y, Wang H, Hu B, Zhang X, Liu A, Cai C, Li S, Chen C, Wang Z, Yin Z, et al. Dendritic Spine in Autism Genetics: Whole-Exome Sequencing Identifying De Novo Variant of CTTNBP2 in a Quad Family Affected by Autism Spectrum Disorder. Children. 2023; 10(1):80. https://doi.org/10.3390/children10010080

Chicago/Turabian Style

Xie, Yingmei, Hui Wang, Bing Hu, Xueli Zhang, Aiping Liu, Chunquan Cai, Shijun Li, Cheng Chen, Zhangxing Wang, Zhaoqing Yin, and et al. 2023. "Dendritic Spine in Autism Genetics: Whole-Exome Sequencing Identifying De Novo Variant of CTTNBP2 in a Quad Family Affected by Autism Spectrum Disorder" Children 10, no. 1: 80. https://doi.org/10.3390/children10010080

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