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Article

Elastomeric Cardiowrap Scaffolds Functionalized with Mesenchymal Stem Cells-Derived Exosomes Induce a Positive Modulation in the Inflammatory and Wound Healing Response of Mesenchymal Stem Cell and Macrophage

1
Laboratory of Biosurgical Research (Alain Carpentier Foundation), Pompidu Hospital, University of Paris, 75015 Paris, France
2
GVM Care & Research, Maria Cecilia Hospital, 48033 Cotignola, Italy
3
Department of UMR, University Sorbonne Paris Nord, 93430 Villetaneuse, France
4
Translational Medicine Department, University of Ferrara, 44123 Ferrara, Italy
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Department of Neurosciences, University of Padova, 35133 Padova, Italy
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Division of Internal Medicine, St. Anna Hospital, 44123 Ferrara, Italy
7
Department of Medical Sciences, University of Ferrara, 44123 Ferrara, Italy
*
Author to whom correspondence should be addressed.
Equal contribution.
Academic Editors: Robert Passier and Raffaele Pugliese
Biomedicines 2021, 9(7), 824; https://doi.org/10.3390/biomedicines9070824
Received: 3 May 2021 / Revised: 7 July 2021 / Accepted: 7 July 2021 / Published: 15 July 2021
(This article belongs to the Special Issue Tissue Engineering in Cardiology)
A challenge in contractile restoration of myocardial scars is one of the principal aims in cardiovascular surgery. Recently, a new potent biological tool used within healing processes is represented by exosomes derived from mesenchymal stem cells (MSCs). These cells are the well-known extracellular nanovesicles released from cells to facilitate cell function and communication. In this work, a combination of elastomeric membranes and exosomes was obtained and tested as a bioimplant. Mesenchymal stem cells (MSCs) and macrophages were seeded into the scaffold (polycaprolactone) and filled with exosomes derived from MSCs. Cells were tested for proliferation with an MTT test, and for wound healing properties and macrophage polarization by gene expression. Moreover, morphological analyses of their ability to colonize the scaffolds surfaces have been further evaluated. Results confirm that exosomes were easily entrapped onto the surface of the elastomeric scaffolds, increasing the wound healing properties and collagen type I and vitronectin of the MSC, and improving the M2 phenotype of the macrophages, mainly thanks to the increase in miRNA124 and decrease in miRNA 125. We can conclude that the enrichment of elastomeric scaffolds functionalized with exosomes is as an effective strategy to improve myocardial regeneration. View Full-Text
Keywords: heart failure; myocardial infarction; cardiac tissue engineering; elastomeric scaffold; cardiopatch; cardiowrap; polycaprolactone; mesenchymal stem cells; exosomes heart failure; myocardial infarction; cardiac tissue engineering; elastomeric scaffold; cardiopatch; cardiowrap; polycaprolactone; mesenchymal stem cells; exosomes
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MDPI and ACS Style

Chachques, J.C.; Gardin, C.; Lila, N.; Ferroni, L.; Migonney, V.; Falentin-Daudre, C.; Zanotti, F.; Trentini, M.; Brunello, G.; Rocca, T.; Gasbarro, V.; Zavan, B. Elastomeric Cardiowrap Scaffolds Functionalized with Mesenchymal Stem Cells-Derived Exosomes Induce a Positive Modulation in the Inflammatory and Wound Healing Response of Mesenchymal Stem Cell and Macrophage. Biomedicines 2021, 9, 824. https://doi.org/10.3390/biomedicines9070824

AMA Style

Chachques JC, Gardin C, Lila N, Ferroni L, Migonney V, Falentin-Daudre C, Zanotti F, Trentini M, Brunello G, Rocca T, Gasbarro V, Zavan B. Elastomeric Cardiowrap Scaffolds Functionalized with Mesenchymal Stem Cells-Derived Exosomes Induce a Positive Modulation in the Inflammatory and Wound Healing Response of Mesenchymal Stem Cell and Macrophage. Biomedicines. 2021; 9(7):824. https://doi.org/10.3390/biomedicines9070824

Chicago/Turabian Style

Chachques, Juan C., Chiara Gardin, Nermine Lila, Letizia Ferroni, Veronique Migonney, Celine Falentin-Daudre, Federica Zanotti, Martina Trentini, Giulia Brunello, Tiberio Rocca, Vincenzo Gasbarro, and Barbara Zavan. 2021. "Elastomeric Cardiowrap Scaffolds Functionalized with Mesenchymal Stem Cells-Derived Exosomes Induce a Positive Modulation in the Inflammatory and Wound Healing Response of Mesenchymal Stem Cell and Macrophage" Biomedicines 9, no. 7: 824. https://doi.org/10.3390/biomedicines9070824

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