Review Reports

Round 1

Reviewer 1 Report

This is a very interesting manuscript on the mitotane and its interaction/action on the estrogen receptor. The manuscript raises an important clinical problem and approaches it both via structural chemical and in vitro pharmacological cell line experiments. The manuscript is well-written, easy to follow, and the applied methodology, results, discussion and the conclusions are clear. The results have clear clinical relevance, as they seem to explain clinical findings, and can form the basis of further translational studies that could eventually lead to better compounds in the combat against adrenocortical cancer.

Author Response

We thank very much the Reviewer for his/her comments and we are glad he/she appreciated our work

Reviewer 2 Report

Only editorial revisions will be needed.

1. page 1, line 3 of ABSTRACT, page 2, line 18: Estrogen Receptor-α should be estrogen receptor-α
2. Page 2, line 16: 17β-estradiol should be 17β-estradiol (E2), after this, E2 should be used instead of 17β-estradiol in the text and figure legends
3. page 2, line 22: ER-α and Estrogen Receptor-β (ER-β) should be ER-α and ER-β
4. page 4, line 10: ThermoFisher scientific should be ThermoFisher Scientific
5. page 4, line 15: add a space after 24
6. page 4, line 17: hour should be h
7. page 4, line 23: 3-(4,5-Dimethyl... should be 3-(4,5-dimethyl...
8. page 4, line 36: hour should be h
9. page 4, line 43: add a space after 6
10. page 4, line 48: hours should be h
11. page 5, line 10: hour should be h
12. page 8, line 18: add a space after 24
13. page 9, line 2: hour should be h
14. page 9, line 7: add a space after 24
15. page 9, line 11: hours should be h
16. References no. 3, 5, 7, 36, 40, 43: titles should be small capital

Author Response

We thank very much the Reviewer for his/her comments.

We corrected the Ms in each point he/she suggested.

Further, I added the note: M.T. and A.A. are enrolled in the PhD Program on Precision Medicine, University of Brescia